当前共找到 1275 篇文献分享,本页显示第 161 - 180 篇。
161.
白鸟
(2024-12-31 14:20):
#paper doi:10.1038/s41588-024-01819-2 Mapping spatially resolved transcriptomes in human and mouse pulmonary fibrosis.
这是一篇肺纤维化的空间转录组文献。文献基于前人单细胞文献的结果,对人患者IPF和小鼠BLM的肺部病变组织取样,分析病理纤维化生态位,探讨病变机制问题。
单细胞文献发现病变跟KRT5−/KRT17+基底细胞样 (AbBa) 上皮细胞群,Krt8+肺泡分化中间 (ADI) 细胞群有关。
文献的两个分析点是:
1.空转的分析非完全创新,而是基于单细胞的两类病变细胞群,在空间分析两类细胞与纤维化的关联;
2.Visium空转的一个鸡肋问题是:spot的细胞类型是混沌的,即使用单细胞的解卷积推测出来,也很难说明其准确性。
文献的核心是NMF非负矩阵因子,单个spot包含三层定义:NMF因子,手动病理注释,解卷积细胞类型推断;证明三层定义的一致性;能较好的规避单用解卷积推断偏差;后续的分析在此基础上进一步推断...
Nature Genetics,
2024-8.
DOI: 10.1038/s41588-024-01819-2
Abstract:
AbstractIdiopathic pulmonary fibrosis (IPF) is a progressive lung disease with poor prognosis and limited treatment options. Efforts to identify effective treatments are thwarted by limited understanding of IPF pathogenesis and …
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AbstractIdiopathic pulmonary fibrosis (IPF) is a progressive lung disease with poor prognosis and limited treatment options. Efforts to identify effective treatments are thwarted by limited understanding of IPF pathogenesis and poor translatability of available preclinical models. Here we generated spatially resolved transcriptome maps of human IPF (n = 4) and bleomycin-induced mouse pulmonary fibrosis (n = 6) to address these limitations. We uncovered distinct fibrotic niches in the IPF lung, characterized by aberrant alveolar epithelial cells in a microenvironment dominated by transforming growth factor beta signaling alongside predicted regulators, such as TP53 and APOE. We also identified a clear divergence between the arrested alveolar regeneration in the IPF fibrotic niches and the active tissue repair in the acutely fibrotic mouse lung. Our study offers in-depth insights into the IPF transcriptional landscape and proposes alveolar regeneration as a promising therapeutic strategy for IPF.
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162.
钟鸣
(2024-12-31 12:59):
#paper doi:10.1128/iai.00346-24 A newly developed oral infection mouse model of shigellosis for immunogenicity and protective efficacy studies of a candidate vaccine
志贺氏菌感染引发消化系统疾病,对发展中国家构成重大的公共卫生挑战,本文开发了第一个通过口腔攻毒志贺氏菌建立感染的小鼠模型,方式是腹膜内注射去铁胺和氯化铁。作者通过攻毒,从大体病变、免疫系统改变、病理症状等角度验证了该模型的有效性及参考价值。随后还使用该模型验证了志贺氏菌侵袭蛋白侵袭质粒抗原 B (IpaB) 重组蛋白疫苗及嵌合重组疫苗 (rIpaB–T2544)(T2544是沙门氏菌外膜蛋白)的保护效果。
Infection and Immunity,
2024-12-18.
DOI: 10.1128/iai.00346-24
Abstract:
ABSTRACT Shigella infection poses a significant public health challenge in the developing world. However, lack of a widely available mouse model that replicates human shigellosis creates a major bottleneck to …
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ABSTRACT Shigella infection poses a significant public health challenge in the developing world. However, lack of a widely available mouse model that replicates human shigellosis creates a major bottleneck to better understanding of disease pathogenesis and development of newer drugs and vaccines. BALB/c mice pre-treated with streptomycin and iron (FeCl 3 ) plus desferrioxamine intraperitoneally followed by oral infection with virulent Shigella flexneri 2a resulted in diarrhea, loss of body weight, bacterial colonization and progressive colitis characterized by disruption of epithelial lining, loss of crypt architecture with goblet cell depletion, increased polymorphonuclear infiltration into the mucosa, submucosal swelling (edema), and raised proinflammatory cytokines and chemokines in the large intestine. To evaluate the usefulness of the model for vaccine efficacy studies, mice were immunized intranasally with a recombinant protein vaccine containing Shigella invasion protein invasion plasmid antigen B (IpaB). Vaccinated mice conferred protection against Shigella , indicating that the model is suitable for testing of vaccine candidates. To protect both Shigella and Salmonella , a chimeric recombinant vaccine (rIpaB–T2544) was developed by fusing IpaB with Salmonella outer membrane protein T2544. Vaccinated mice developed antigen-specific serum IgG and IgA antibodies and a balanced Th1/Th2 response and were protected against oral challenge with Shigella ( S. flexneri 2a , Shigella dysenteriae , and Shigella sonnei ) using our present mouse model and Salmonella ( Salmonella Typhi and Paratyphi) using an iron overload mouse model. We describe here the development of an oral S higella infection model in wild-type mouse. This model was successfully used to demonstrate the immunogenicity and protective efficacy of a candidate protein subunit vaccine against Shigella .
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163.
小年
(2024-12-31 11:46):
#paper doi:10.1038/s41467-022-29336-y
Distinct genomic landscape of Chinese pediatric acute myeloid leukemia impacts clinical risk classification. Nat Commun 13, 1640 (2022).
研究团队进行了中国儿童AML全基因组和转录组学研究,分析了驱动因子(突变特征)改变及其预后相关性,发现中国和西方儿童AML患者的基因突变谱存在显著差异。同时,研究人员提出了一种改进的预后风险分类模型,能够更好地反映中国儿童AML患者的不良事件风险。该研究强调了群体遗传背景在精准医学中的重要性。
Nature Communications,
2022-3-28.
DOI: 10.1038/s41467-022-29336-y
Abstract:
AbstractStudies have revealed key genomic aberrations in pediatric acute myeloid leukemia (AML) based on Western populations. It is unknown to what extent the current genomic findings represent populations with different …
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AbstractStudies have revealed key genomic aberrations in pediatric acute myeloid leukemia (AML) based on Western populations. It is unknown to what extent the current genomic findings represent populations with different ethnic backgrounds. Here we present the genomic landscape of driver alterations of Chinese pediatric AML and discover previously undescribed genomic aberrations, including the XPO1-TNRC18 fusion. Comprehensively comparing between the Chinese and Western AML cohorts reveal a substantially distinct genomic alteration profile. For example, Chinese AML patients more commonly exhibit mutations in KIT and CSF3R, and less frequently mutated of genes in the RAS signaling pathway. These differences in mutation frequencies lead to the detection of previously uncharacterized co-occurring mutation pairs. Importantly, the distinct driver profile is clinical relevant. We propose a refined prognosis risk classification model which better reflected the adverse event risk for Chinese AML patients. These results emphasize the importance of genetic background in precision medicine.
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164.
庞庞
(2024-12-29 21:58):
#paper doi:
DOI: 10.1038/nn.4497 Dynamic models of large-scale brain activity 一篇对大脑动力学模型进行综述的文章,对于我这个初步接触该领域非常有用。文章首先简单介绍了动力学模型中的一些基本概念,比如state, attractors等等;然后介绍了如何对群体神经元进行建模:目前常基于NMM建模,然后根据结构关系(dmri数据)将其耦合到大脑介观尺度;最后探讨了如何验证建模的正确性。
Nature Neuroscience,
2017-3.
DOI: 10.1038/nn.4497
Abstract:
No abstract available.
165.
尹志
(2024-12-29 21:50):
#paper doi: 10.1021/acs.jcim.4c01107 Journal of Chemical Information and Modeling, 2024, Diffusion Models in De Novo Drug Design。又是一篇diffusion model用于药物设计的综述,很新很全面。这个领域的发展特别快,各种方法层出不穷,有了诺奖这次的加持,应该后面几年会有更多务实的成果。
Journal of Chemical Information and Modeling,
2024-10-14.
DOI: 10.1021/acs.jcim.4c01107
Abstract:
No abstract available.
166.
徐炳祥
(2024-12-29 17:05):
#paper doi: 10.1186/s12889-024-21045-z BMC Public Health, 2024, Effects of physical exercise on cardiorespiratory health of young adults during short-term exposure to varying air pollution levels。众所周知,暴露于空气污染环境下会损伤人体心肺机能,而开展体力活动有助于增强人体心肺机能,于是一个有意义的问题是这二者之间的交互作用为何,抑或在何种空气污染程度下进行体力活动可以抵消空气污染本身对心肺机能的影响。为此,本研究招募了30名健康青壮年志愿者在空气污染轻微、中等和严重三个级别下进行了一次性有氧运动训练,并从多个维度评定并对比了训练前后参与者的心肺机能情况。结果显示,在PM2.5≤115的轻度至中等污染环境中进行一小时中等强度有氧训练带给参与者心肺机能的收益足以覆盖暴露于此环境对心肺机能的损害,而在此之上的高浓度污染环境下则不能。本研究说明对健康青壮年,即使在空气不甚洁净时进行户外运动对心肺机能总体上仍然是安全的。
167.
李翛然
(2024-12-27 13:05):
#paper DOI: 10.1093/database/baaa102 NPBS database: a chemical data resource with relational data between natural products and biological sources. Database 2020, baaa102. 一个天然产物数据库,最近我在研究天然产物的一些方案,发现上海药物所把世界上主要的天然产物搞了个数据集,这个还是不错的。给大家也推荐一下, 但是搜索还是没做好,还是字符串相似度搜索,拉丁翻译和中文对应的比较差劲。 应该上Vector search了。
Database,
2020-12-11.
DOI: 10.1093/database/baaa102
Abstract:
Abstract NPBS (Natural Products & Biological Sources) database is a chemical data resource with relational data between natural products and biological sources, manually curated from literatures of natural product researches. …
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Abstract NPBS (Natural Products & Biological Sources) database is a chemical data resource with relational data between natural products and biological sources, manually curated from literatures of natural product researches. The relational data link a specific species and all the natural products derived from it and contrarily link a specific natural product and all the biological sources. The biological sources cover diverse species of plant, bacterial, fungal and marine organisms; the natural molecules have proper chemical structure data and computable molecular properties and all the relational data have corresponding references. NPBS database provides a wider choice of biological sources and can be used for dereplication to prevent re-isolation and re-characterization of already known natural products. Database URL: http://www.organchem.csdb.cn/scdb/NPBS
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168.
颜林林
(2024-12-22 16:33):
#paper doi:10.1038/s41593-024-01812-2, Nature Neuroscience, The cell-type underpinnings of the human functional cortical connectome. 这篇文章于上月发表在《Nature Neuroscience》,结合 fMRI 和单细胞转录组测序数据,研究大脑皮层功能梯度与细胞类型分布的关系。fMRI 通常用于从宏观上研究大脑功能,而转录组等基于高通量测序的技术则太过微观,难以直接对应到宏观功能上。我最近因参与的课题,需要将基因组学与脑影像学结合起来,因此对这个方向做了一些文献调研,但发现绝大多数文章在将fMRI和组学数据进行关联时都浅尝辄止,所幸找到这篇文章在这方面的分析相对深入,因此给了我一定的启发。他们的 fMRI 数据来自 Human Connectome Project(HCP),而单核 RNA 测序(snRNA-seq)数据来自 Allen 人脑图谱(AHBA)。通过将 fMRI 数据得到的功能网络,映射成为功能梯度(functional gradients),功能梯度反映了从单模态(如视觉)到跨模态(如默认网络)的连续变化;再利用反卷积方法分析转录组数据,推断出每个皮层分区的细胞组成;之后通过空间坐标将两者对齐并联系起来,从而可以对不同功能梯度的细胞组成分布变化关系进行详细讨论。这篇文章的数据和方法都是公开的,值得仔细研究下其方法细节。
169.
龙海晨
(2024-12-09 13:52):
#paper Järve M, Saag L, Scheib CL, Pathak AK, Montinaro F, Pagani L, Flores R, Guellil M, Saag L, Tambets K, Kushniarevich A, Solnik A, Varul L, Zadnikov S, Petrauskas O, Avramenko M, Magomedov B, Didenko S, Toshev G, Bruyako I, Grechko D, Okatenko V, Gorbenko K, Smyrnov O, Heiko A, Reida R, Sapiehin S, Sirotin S, Tairov A, Beisenov A, Starodubtsev M, Vasilev V, Nechvaloda A, Atabiev B, Litvinov S, Ekomasova N, Dzhaubermezov M, Voroniatov S, Utevska O, Shramko I, Khusnutdinova E, Metspalu M, Savelev N, Kriiska A, Kivisild T, Villems R. Shifts in the Genetic Landscape of the Western Eurasian Steppe Associated with the Beginning and End of the Scythian Dominance. Curr Biol. 2019 Jul 22;29(14):2430-2441.e10. doi: 10.1016/j.cub.2019.06.019. Epub 2019 Jul 11. PMID: 31303491. 这是一篇DNA检测与考古相结合的文章,文章对欧洲西部草原古代人类DNA进行研究,根据古代 DNA 证据,铁器时代早期的游牧斯基泰人被描述为不同起源的部落联盟 。研究提供了 31 个古代西方和东方草原个体的新全基因组序列,包括斯基泰人以及他们之前和之后的样本,在斯基泰人统治初期的铁器时代早期滨海基因库中检测到东部(阿尔泰)亲和力的增加以及东部狩猎采集者血统的减少。乌克兰斯基泰人之后的切尔尼亚希夫文化样本中近东血统的比例明显高于本研究的其他样本。基因构成与后斯基泰切尔尼亚希夫文化的哥特式起源一致。
170.
惊鸿
(2024-12-02 21:14):
#paper Topological semimetals with intrinsic chirality as spin-controlling electrocatalysts for the oxygen evolution reaction. Published: 25 November 2024
[https://doi.org/10.1038/s41560-024-01674-9](https://doi.org/10.1038/s41560-024-01674-9)
这篇文章是由德国马克斯·普朗克固体研究所(Max Planck Institute for Solid State Research)的科学家们主导的研究工作。研究团队报告了一系列具有内在手性的拓扑半金属,这些材料能够有效地进行水分解。特别是,基于Rh的拓扑单晶(RhSi、RhSn和RhBiS)展现了手性、大的自旋-轨道耦合(SOC)和良好的导电性这三个关键特性。这些Rh基手性晶体作为OER催化剂在碱性电解质中展现出比RuO2显著增强的OER活性。实验和量子输运计算表明,Rh基手性晶体的OER活性与SOC和自旋极化成正比。其中,具有最大SOC的RhBiS即使在100 mA cm–2的电流密度下也展现出266 mV的过电位,性能超过了具有相当大表面积的最先进的纳米结构催化剂。该研究为设计高性能的内在手性OER催化剂提供了实验证据,证实了自旋放大OER与SOC之间的密切关系。
Nature Energy,
2024-11-25.
DOI: 10.1038/s41560-024-01674-9
Abstract:
AbstractElectrocatalytic water splitting is a promising approach for clean hydrogen production, but the process is hindered by the sluggish kinetics of the anodic oxygen evolution reaction (OER) owing to the …
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AbstractElectrocatalytic water splitting is a promising approach for clean hydrogen production, but the process is hindered by the sluggish kinetics of the anodic oxygen evolution reaction (OER) owing to the spin-dependent electron transfer process. Efforts to control spin through chirality and magnetization have shown potential in enhancing OER performance. Here we harnessed the potential of topological chiral semimetals (RhSi, RhSn and RhBiS) and their spin-polarized Fermi surfaces to promote the spin-dependent electron transfer in the OER, addressing the traditional volcano-plot limitations. We show that OER activities follow the trend RhSi < RhSn < RhBiS, corresponding to the increasing extent of spin–orbit coupling (SOC). The chiral single crystals outperform achiral counterparts (RhTe2, RhTe and RuO2) in alkaline electrolyte, with RhBiS exhibiting a specific activity two orders of magnitude higher than RuO2. Our work reveals the pivotal roles of chirality and SOC in spin-dependent catalysis, facilitating the design of ultra-efficient chiral catalysts.
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171.
刘昊辰
(2024-12-01 20:41):
#paper Connect6 Opening Leveraging AlphaZero Algorithm and Job-Level Computing. 这是一篇基于 AlphaZero算法和作业级计算构建 Connect6 开局库的方法的研究论文。开局库构建可提升棋类程序实力,在限时比赛中优势明显。过去依赖游戏特定知识构建开局库,存在质量依赖人类知识、方法难以通用等问题。本文提出基于 AlphaZero 构建高质量开局库的方法,不依赖领域知识。实验和比赛证明,该开局库可提升 Connect6 程序实力,在常见开局位置表现良好,助力程序在实际比赛中夺冠。本文提出的方法为构建高质量开局库提供了新思路,有望在其他棋类游戏中得到应用和推广。下载地址:https://www.jstage.jst.go.jp/article/pjsai/JSAI2021/0/JSAI2021_4N4IS1c05/_pdf/-char/ja
Abstract:
No abstract available.
172.
惊鸿
(2024-12-01 20:18):
#paper Integrated Carbon Dioxide Capture by Amines and Conversion to Methane on Single-Atom Nickel Catalysts Pub Date : 2024-11-06 , DOI: 10.1021/jacs.4c09744 二氧化碳 (CO2) 捕获物质(即氨基甲酸酯和(氢)碳酸盐)的直接电化学还原有望从点源捕获和转化 CO2,其中绕过了对能量要求高的汽提步骤。在这里,我们描述了一类电沉积在各种电极表面上的原子分散的镍 (Ni) 催化剂,这些催化剂被证明可以直接将捕获的 CO2 转化为甲烷 (CH4)。采用 X 射线光电子能谱 (XPS) 和电子显微镜 (EM) 的详细研究表明,高度分散的 Ni 原子对于将捕获物质转化为 CH4 具有独特的活性,并且单原子 Ni 的活性是通过使用分子定义的 Ni 酞菁催化剂的对照实验证实的碳纳米管负载。比较从氢氧化物、氨、伯胺、仲胺和叔胺获得的各种捕获溶液的动力学,可以证明氨基甲酸酯而不是(氢)酸盐和/或溶解的 CO2 是产生 CH4 的主要原因。这一结论得到了捕获溶液的 13C 核磁共振 (NMR) 波谱以及比较有和没有 CO2 吹扫的反应选择性的对照实验的支持。这些发现在密度泛函理论 (DFT) 计算的帮助下得到了理解,该计算表明分散在金 (Au) 上的单原子镍 (Ni) 对氨基甲酸酯的直接还原具有活性,产生 CH4 作为主要产物。 这是氨基甲酸酯直接电化学转化为 CH4 的第一个例子,该过程的机制为综合捕获 CO2 直接转化为碳氢化合物的潜力提供了新的见解。
Journal of the American Chemical Society,
2024-11-20.
DOI: 10.1021/jacs.4c09744
Abstract:
No abstract available.
173.
DeDe宝
(2024-12-01 16:21):
#paper, Impact of conflicts between long- and short-term priors on the weighted prior integration in visual perception, doi:10.1016/j.cognition.2024.106006, 本研究探讨了长期和短期先验冲突对视觉感知中加权先验整合的影响。研究要求被试估计Gabor的朝向,并提出了一个贝叶斯观察者模型,该模型预测了视觉系统通过加权平均来整合长期和短期先验,两种先验之间的冲突程度决定了它们在整合先验中的相对权重。随着长期和短期先验之间冲突程度的增加,新先验中短期先验的权重会降低。模型假设了两种先验:长期先验是被试固有的,不受训练影响的,对正交方向敏感的先验。短期先验是受到实验目标分布影响的先验。实验分布一共有三种:自然分布(正交方向居多,符合自然情况分布规律)、反自然分布(倾斜方向居多)和自然分布但振幅增加。行为结果显示,被试在不同分布中Gabor朝向时都存在正交偏差(cardinal bias),且反自然分布的朝向更倾向于长期先验的方向。先验分布影响被试的偏差,但不影响标准差。行为结果支持了研究提出的加权先验整合模型。这个研究的亮点在于区分了短期和长期的先验,并揭示了感知系统中长期和短期先验的加权整合机制。
174.
前进
(2024-11-30 23:26):
# paper DOI: [10.1093/bib/bbv088](https://doi.org/10.1093/bib/bbv088) A Comparison of Base-calling Algorithms for Illumina Sequencing Technology
这篇论文主要讲述了Illumina测序技术中用于basecalling的不同算法的性能比较。文章提供了一个综合的比较分析,涵盖了多种最近开发的bascalling算法,并提出了一个统一的统计模型,该模型能够涵盖大多数现有的basecall算法。研究的目的在于通过比较这些算法在处理Illumina平台产生的测序数据时的准确性和效率,来帮助科研人员选择最适合他们需求的basecall工具。论文中提到的算法包括Bustard、Srfim、AYB、Ibis和freeIbis等,并通过实验数据评估了它们的对齐率、错误率和区分能力。通过这些比较,论文旨在为高通量测序数据分析中basecall步骤提供指导和建议。
Briefings in Bioinformatics,
2016-9.
DOI: 10.1093/bib/bbv088
Abstract:
No abstract available.
175.
钟鸣
(2024-11-30 23:20):
#paper doi:10.1016/j.envint.2023.108349 本文作者通过两阶段的富集过程,从超级蠕虫的肠道微生物群落中建立了稳定且可复制的塑料相关生物膜群落。第一阶段是让蠕虫食用塑料,第二阶段是对食用塑料的蠕虫的肠道微生物群落进行体外孵化。研究结果表明塑料喂养对细菌多样性的影响较小,但显著改变了不同细菌群的相对丰度,并富集了潜在的塑料降解菌。在体外孵化过程中,微生物群落的更显著变化,塑料降解菌被进一步富集,而乳酸菌等其他菌类则减少。塑料特性分析证实了塑料相关微生物群落对孵化塑料的降解作用。通过基因丰度和群落表型的社区功能推断,体外孵化增强了塑料降解潜力。
Environment International,
2024-1.
DOI: 10.1016/j.envint.2023.108349
Abstract:
No abstract available.
176.
林海onrush
(2024-11-30 22:18):
#paper, 《Three-manifolds with positive Ricci curvature》, doi: 10.4310/jdg/1214436922, 理查德·S·汉密尔顿(Richard S. Hamilton)于1982年发表的论文, 主要研究了三维流形在正Ricci曲率条件下的几何演化规律。通过引入Ricci流这一核心工具,即一种类似热方程的演化方程,证明了若一个紧致三维流形具有严格正的Ricci曲率,则该性质在Ricci流的演化过程中会始终保持,并最终收敛到恒定正曲率的度量。这一结果解决了Bourguignon提出的关于正Ricci曲率流形分类的猜想,并进一步明确了正曲率三维流形的几何结构特性。为此,作者运用了Nash-Moser反演函数定理来处理非严格抛物性演化方程的解,同时结合最大值原理与插值不等式,确保了解的长期存在性和收敛性。
论文的创新在于巧妙地简化了三维情况下的几何分析问题,通过Ricci曲率直接推导完整的曲率张量,大大降低了计算复杂度。文章展示了三维流形中Ricci流的稳定性与长期行为,不仅为流形几何研究提供了重要工具,也为拓扑学领域的经典问题(如Poincaré猜想)提供了新的视角。虽然本文集中于三维流形,但所用方法和理论工具也可能适用于更高维度流形的研究,从而具有广泛的学术意义和应用潜力。
备注(引用维基百科):里奇-哈密顿流,一般称为里奇流(Ricci flow)在微分几何中是指一种固有的几何学流动,它的主要思想是让流形随时间变形,即是让度规张量随时间变化,观察在流形的变形下,里奇曲率是如何变化的,以此来研究整体的拓扑性质。它的核心是里奇-哈密顿流方程,是一个拟线性抛物型方程组。
里奇流以意大利数学家格雷戈里奥·里奇-库尔巴斯托罗的名字命名,由美国数学家理查德·哈密顿于1981年首次引入。这个工具同时被俄罗斯数学家格里戈里·佩雷尔曼用于解决千禧年大奖难题之一的庞加莱猜想。同样的,西蒙·布伦德和理查德·肖恩正是使用它,使微分球面定理完成证明。
Journal of Differential Geometry,
1982-1-1.
DOI: 10.4310/jdg/1214436922
Abstract:
No abstract available.
177.
盼盼
(2024-11-30 22:11):
#paper doi:10.1101/2021.04.29.441889 Type I interferon responsive microglia shape cortical development and behavior. 在Cell杂志发表的这篇文章,研究人员发现了一类I型干扰素(IFN-Ⅰ)响应性的小胶质细胞亚群,这一类群的细胞在皮层重塑期间高度活跃,可发挥吞噬神经元的作用,而在正常大脑发育过程中罕见。实验结果还表明,IFN-Ⅰ缺陷小鼠在触觉刺激时表现出更高的敏感性,这说明IFN-Ⅰ可能参与调节触觉反应。总之,这一类独特的小胶质细胞在大脑皮层的发育和感觉功能中发挥重要作用,这些发现为理解IFN-Ⅰ在大脑对多种损伤反应过程中的作用提供了新的视角。
bioRxiv,
2023-3-20.
DOI: 10.1101/2021.04.29.441889
Abstract:
SummaryMicroglia are brain resident phagocytes that can engulf synaptic components and extracellular matrix as well as whole neurons. However, whether there are unique molecular mechanisms that regulate these distinct phagocytic …
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SummaryMicroglia are brain resident phagocytes that can engulf synaptic components and extracellular matrix as well as whole neurons. However, whether there are unique molecular mechanisms that regulate these distinct phagocytic states is unknown. Here we define a molecularly distinct microglial subset whose function is to engulf neurons in the developing brain. We transcriptomically identified a cluster of Type I interferon (IFN-I) responsive microglia that expanded 20-fold in the postnatal day 5 somatosensory cortex after partial whisker deprivation, a stressor that accelerates neural circuit remodeling.In situ, IFN-I responsive microglia were highly phagocytic and actively engulfed whole neurons. Conditional deletion of IFN-I signaling (Ifnar1fl/fl) in microglia but not neurons resulted in dysmorphic microglia with stalled phagocytosis and an accumulation of neurons with double strand DNA breaks, a marker of cell stress. Conversely, exogenous IFN-I was sufficient to drive neuronal engulfment by microglia and restrict the accumulation of damaged neurons. IFN-I deficient mice had excess excitatory neurons in the developing somatosensory cortex as well as tactile hypersensitivity to whisker stimulation. These data define a molecular mechanism through which microglia engulf neurons during a critical window of brain development. More broadly, they reveal key homeostatic roles of a canonical antiviral signaling pathway in brain development.
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178.
尹志
(2024-11-30 22:05):
#paper https://doi.org/10.48550/arXiv.1701.08223 2017, The Python-based Simulations of Chemistry Framework (PySCF)。非常重要的量子化学工具PySCF的介绍。2014年启动的项目,从一开始的仅仅有几个函数功能,到现在对各种量化问题的计算的良好支持,其易用性及可扩展性得到了社群的认可。这个特性其实在软件于2015年发布的时候就设定好了。因此,几乎所有功能代码都由python实现,只有遇到特别的time-ciritical的代码部分才去用c实现。当然,这个特性使得目前大量量化计算的库都依赖于pyscf,俨然成为开源领域的gaussion的有力竞争者。
arXiv,
2017-01-27T23:57:43Z.
DOI: 10.48550/arXiv.1701.08223
Abstract:
PySCF is a general-purpose electronic structure platform designed from theground up to emphasize code simplicity, both to aid new method development, aswell as for flexibility in computational workflow. The package …
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PySCF is a general-purpose electronic structure platform designed from theground up to emphasize code simplicity, both to aid new method development, aswell as for flexibility in computational workflow. The package provides a widerange of tools to support simulations of finite size systems, extended systemswith periodic boundary conditions, low dimensional periodic systems, and customHamiltonians, using mean-field and post-mean-field methods with standardGaussian basis functions. To ensure easy of extensibility, PySCF uses thePython language to implement almost all its features, while computationallycritical paths are implemented with heavily optimized C routines. Using thiscombined Python/C implementation, the package is as efficient as the bestexisting C or Fortran based quantum chemistry programs. In this paper wedocument the capabilities and design philosophy of the current version of thePySCF package.
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179.
muton
(2024-11-30 21:53):
#paper An abstract relational map emerges in the human medial prefrontal cortex with consolidation. doi: https://doi.org/10.1101/2024.10.11.617652 我们在面对一个问题时,如果能够识别问题背后的结构,那么对于解决这个问题,以及面对类似问题时候的泛化是及其有帮助的。最近的理论研究强调了与感官细节完全分离的显式结构表征对于泛化的有用性。啮齿动物的研究表明,这种结构的抽象是逐渐发生的,随着时间的推移在大脑皮层中进行。然而,在人类中这种显式关系表征的直接证据很少,它与巩固机制的关系尚未研究清楚。因此,作者使用一种图形学习的范式在人类内侧前额叶皮质中找到了这样一个关系图。重要的是,这种表征在学习后早期并没有表现出来,但在几天后却出现了。说明这种抽象的关系结构图是逐渐形成并抽象化的,并且最终存储在内侧前额叶。这一结果为理解人类强大的推理能力提供了新的视角。
bioRxiv,
2024-10-11.
DOI: 10.1101/2024.10.11.617652
Abstract:
AbstractUnderstanding the structure of a problem, such as the relationships between stimuli, supports fast learning and flexible reasoning. Recent theoretical suggestions have highlighted the usefulness of explicit structural representations that …
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AbstractUnderstanding the structure of a problem, such as the relationships between stimuli, supports fast learning and flexible reasoning. Recent theoretical suggestions have highlighted the usefulness of explicit structural representations that are fully divorced from sensory details for generalisation. Rodent work has suggested that abstraction of structure occurs gradually, over time, in cortex. However, direct evidence of such explicit relational representations in humans is scarce, and its relationship to consolidation mechanisms is underexplored. Here, we use a graph-learning paradigm to find such a relational map in the human medial prefrontal cortex. Importantly, this representation was absent early after learning but emerged on the time scale of days. These results shed new light on neural representations underlying the remarkable human ability to draw accurate inferences from little data.
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180.
哪有情可长
(2024-11-30 21:45):
#paper Pan-genome bridges wheat structural variations with habitat and breeding,Nature,doi.org/10.1038/s41586-024-08277-0文章对中国育种过程中具有代表性的17个品种进行从头组装和注释。通过整合17个新组装的基因组和4个先前发表的基因组(CS、Fielder、Kariega和Attraktion)进行泛基因组分析,并通过直系同源基因鉴定,将21个基因组中的基因归类为170,517个潜在基因家族。后续对不同时期(50-60年代、80-90年代和2000年以后)基因组中NLR基因的拷贝数进行分析,发现80-90年代育成审定的小麦品种,如XY6和YM158,具有最多的NLR基因拷贝数。随着时间推移,CC-NBARC-LRR基因的拷贝数略有下降,而其他NLR基因(如NBARC-LRR)的变化不显著。对小麦春冬性基因 VRN-A1和籽粒硬度性状基因PIN基因的结构变异进行分析,发现不同的生态型跟环境和人文饮食习惯相关。该研究一方面扩大了小麦现有的参考基因组,在测序材料选择方便考虑到中国育种过程的材料品种和饮食习惯相结合,文章的故事性更丰富。此外文章关注了小麦育种的抗性蛋白NLR基因,为后续的小麦抗性育种研究提供了宝贵的资源。