当前共找到 19 篇文献分享。
1.
半面阳光
(2025-04-30 22:51):
#paper doi: https://doi.org/10.1016/j.gim.2023.100947. Genetics in Medicine. 2023. The landscape of reported VUS in multi-gene panel and genomic testing: Time for a change. 意义不明变异(Variants of Uncertain Significance,VUS)在基因诊断检测中较为常见,这类变异可能导致临床解读困难,并产生额外的后续成本,包括临床医生额外的时间投入。这篇文章的主要是为了调查多基因panel(Multi-gene Panels,MGPs)和外显子组及基因组测序(Exome and Genome Sequencing,ES/GS)诊断检测报告的VUS结果的发生率,评估不确定结果的影响范围,并探索减少其潜在不良影响的方法。研究收集了2020年至2021年间,北美地区19家临床实验室超过150万份测序检测报告的数据,分析因VUS导致的结果不确定率。结果发现,ES/GS导致的不确定结果(VUS)发生率(22.5%)显著低于MGPs(32.6%;P < .0001)。在MGPs检测中,不确定结果的发生率与检测基因数量呈正相关。与单样本检测相比,家系检测(trios,即父母-子女三人组合)能明显降低不确定结果的发生率(18.9% vs 27.6%;P < .0001),而基因组测序(GS)与外显子组测序(ES)相比在VUS发生率上无显著差异(22.2% vs 22.6%;P = ns)。 在多基因panel(MGPs)的检测中,观察到的VUS高发生率提示需要重新审视现行的变异报告规范。文章提出了一些减少VUS报告率的方法,采用其他方法(如对VUS进行亚分类及提供补充报告信息)或可同时提升两类检测的有效性。文章还根据研究结果的提示,建议将临床资源重点用于对有临床意义的VUS进行进一步跟踪评估。
2.
符毓
(2025-04-30 22:15):
#paper doi: arxiv.org/abs/2504.19193, 2025, Trajectory Planning with Model Predictive Control for Obstacle Avoidance Considering Prediction Uncertainty. 本文介绍了一种用于自主机器人的新型轨迹规划器,在机器人操作系统(ROS2) 和导航框架(Nav2)中融入动态避障功能来增强导航性能。该方法利用模型预测控制 (MPC),重点处理与动态障碍物运动预测相关的不确定性。与主要处理静态障碍物或对动态障碍物当前位置做出反应的现有Nav2轨迹规划器不同,该规划器预测未来障碍物的位置,从而确保机器人避开可能存在障碍物的区间
arXiv,
2025-04-27T11:00:19Z.
DOI: 10.48550/arXiv.2504.19193
Abstract:
This paper introduces a novel trajectory planner for autonomous robots,specifically designed to enhance navigation by incorporating dynamic obstacleavoidance within the Robot Operating System 2 (ROS2) and Navigation 2 (Nav2)framework. The …
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This paper introduces a novel trajectory planner for autonomous robots,specifically designed to enhance navigation by incorporating dynamic obstacleavoidance within the Robot Operating System 2 (ROS2) and Navigation 2 (Nav2)framework. The proposed method utilizes Model Predictive Control (MPC) with afocus on handling the uncertainties associated with the movement prediction ofdynamic obstacles. Unlike existing Nav2 trajectory planners which primarilydeal with static obstacles or react to the current position of dynamicobstacles, this planner predicts future obstacle positions using a stochasticVector Auto-Regressive Model (VAR). The obstacles' future positions arerepresented by probability distributions, and collision avoidance is achievedthrough constraints based on the Mahalanobis distance, ensuring the robotavoids regions where obstacles are likely to be. This approach considers therobot's kinodynamic constraints, enabling it to track a reference path whileadapting to real-time changes in the environment. The paper details theimplementation, including obstacle prediction, tracking, and the constructionof feasible sets for MPC. Simulation results in a Gazebo environmentdemonstrate the effectiveness of this method in scenarios where robots mustnavigate around each other, showing improved collision avoidance capabilities.
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3.
庞庞
(2025-04-30 21:48):
#paper Connectional axis of individual functional variability: Patterns, structural correlates, and relevance for development and cognition www.pnas.org/doi/10.1073/pnas.2420228122
研究揭示功能连接个体差异的空间分布模式,其结构连接基础,及与儿童青少年发育和高级认知的关系。
Proceedings of the National Academy of Sciences,
2025-3-25.
DOI: 10.1073/pnas.2420228122
Abstract:
The human cerebral cortex exhibits intricate interareal functional synchronization at the macroscale, with substantial individual variability in these functional connections. However, the spatial organization of functional connectivity (FC) variability across …
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The human cerebral cortex exhibits intricate interareal functional synchronization at the macroscale, with substantial individual variability in these functional connections. However, the spatial organization of functional connectivity (FC) variability across the human connectome edges and its significance in cognitive development remain unclear. Here, we identified a connectional axis in the edge-level FC variability. The variability declined continuously along this axis from within-network to between-network connections and from the edges linking association networks to those linking the sensorimotor and association networks. This connectional axis of functional variability is associated with spatial pattern of structural connectivity variability. Moreover, the connectional variability axis evolves in youth with an flatter axis slope. We also observed that the slope of the connectional variability axis was positively related to the performance in the higher-order cognition. Together, our results reveal a connectional axis in functional variability that is linked with structural connectome variability, refines during development, and is relevant to cognition.
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4.
哪有情可长
(2025-04-30 21:38):
#paper Reconstructing the Transcriptional Ontogeny of Maize and Sorghum Supports an Inverse Hourglass Model of Inflorescence Development, Current Biology, October 21, 2019. https://doi.org/10.1016/j.cub.2019.08.044.对玉米和高粱的穗构建了不同发育时期的转录组图谱,借助转录本动力学确定了玉米抽穗和高粱穗发育阶段,然后利用随机森林确定了基因表达预测,发现这两个物种在将干细胞命运方面最相似。这个工作对我最近分析多物种的比较转录组很有启发。
5.
白鸟
(2025-04-30 21:37):
#paper doi:10.1016/j.immuni.2023.10.013 Single-cell atlas of healthy human blood unveils age-related loss of NKG2C+GZMB−CD8+ memory T cells and accumulation of type 2 memory T cells
文章调研了166名25-85岁人群的200万个PBMC的临床数据和单细胞测序数据;通过分析317个样本55个细胞亚群,寻找健康人群的血液关于年龄相关的变化趋势,发现NKG2C+GZMB−CD8+记忆T细胞亚群随年龄显著减少;随着年龄的增长,2型记忆性CD4+和CD8+T细胞增加,并能产生更多的IL-4。这是一篇从单细胞水平研究免疫年龄,研究人群免疫年龄的共性,寻找免疫在人体衰老过程中的变化。也有不少模型对免疫年龄进行预测,真实性准确性有待商榷。
6.
Vincent
(2025-04-30 21:21):
#paper https://doi.org/10.1038/s41467-025-57430-4 DrBioRight 2.0: an LLM-powered bioinformatics chatbot for large-scale cancer functional proteomics analysis, nature communications, 2025. 该研究介绍了由大型语言模型驱动的生物信息学平台 DrBioRight 2.0,旨在以自然语言交互方式支持大规模癌症功能蛋白质组学分析。研究团队整合了来自 TCGA 和 CCLE 的约 9000 个样本,使用近 500 种抗体构建了涵盖主要癌症信号通路的蛋白质组学数据库。DrBioRight 2.0 通过自然语言指令即可实现跨组学数据的查询、分析与可视化,具备自动代码生成、错误校正、插件式可视化等功能,显著提升了非程序员研究者对复杂蛋白质组学数据的可及性与分析效率。该平台现已上线,网址为 https://drbioright.org。
Nature Communications,
2025-3-6.
DOI: 10.1038/s41467-025-57430-4
Abstract:
No abstract available.
7.
钟鸣
(2025-04-30 20:54):
#paper doi:10.1128/JCM.00329-21 New Pneumococcal Serotype 15D
作者团队通过全基因组测序监测到两株不同的链球菌,他们的荚膜多糖合成基因簇(cps)呈现出独特的结构,其缺失了wcjE基因,并可能因此呈现出不同的荚膜结构。而血清学实验也表明该菌株也确实与其他已知血清型菌株不同。这项研究指引了高通量测序技术在病原微生物分型中的应用方向和价值,同时也提示在研究细菌血清学分型中应关注荚膜多糖合成基因的微进化机制(如基因丢失、移码突变)对血清型转换的影响。
Journal of Clinical Microbiology,
2021-4-20.
DOI: 10.1128/JCM.00329-21
Abstract:
No abstract available.
8.
ZĒNG Yíngzhū (Zoo) 曾莹珠
(2025-04-30 16:49):
#paper
DOI: 10.1126/sciadv.adn5290
SCIENCE ADVANCES
2024
Generative AI enhances individual creativity but reduces the collective diversity of novel content
Reason for recommendation: I don't want to read fear conditioning anymore and don't know what specific topic I want to study in the future. Then I chose the top journal Science and searched CREATIVITY, and found this one that is latest and not very difficult for me to read.
Review:
1, Actually GenAI got popular long time ago but it got common this year, I remember after Spring Festival? I should have read this article earlier.
2, Question1: Five GenAI idea condition 的被试平均只使用了2.55次,只有24.5%的人用了5次。他实验设计的时候就是设置了这一组最多能用5次。我想问为啥不定死这个条件呀?就是规定被试必须用5次,然后再写故事。
3, Question2: for exploratory purposes的这种分析,我看它写在正文和放在补充材料。是不是也可以直接留下用于另写一篇新的呀?假如这篇文章没有这部分,会降低它被Science接收的概率吗……
Science Advances,
2024-7-12.
DOI: 10.1126/sciadv.adn5290
Abstract:
Creativity is core to being human. Generative artificial intelligence (AI)—including powerful large language models (LLMs)—holds promise for humans to be more creative by offering new ideas, or less creative by …
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Creativity is core to being human. Generative artificial intelligence (AI)—including powerful large language models (LLMs)—holds promise for humans to be more creative by offering new ideas, or less creative by anchoring on generative AI ideas. We study the causal impact of generative AI ideas on the production of short stories in an online experiment where some writers obtained story ideas from an LLM. We find that access to generative AI ideas causes stories to be evaluated as more creative, better written, and more enjoyable, especially among less creative writers. However, generative AI–enabled stories are more similar to each other than stories by humans alone. These results point to an increase in individual creativity at the risk of losing collective novelty. This dynamic resembles a social dilemma: With generative AI, writers are individually better off, but collectively a narrower scope of novel content is produced. Our results have implications for researchers, policy-makers, and practitioners interested in bolstering creativity.
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9.
尹志
(2025-04-30 15:56):
#paper doi:10.48550/arXiv.2407.20516, Machine Unlearning in Generative AI: A Survey. 很有意思的方向,应该是翻译机器遗忘吧。随着模型越做越大,如何通过对模型的处理达到可控的添加与擦除特定信息,是未来一个重要的主题,不管是从隐私保护还是模型控制的层面上
arXiv,
2024-07-30T03:26:09Z.
DOI: 10.48550/arXiv.2407.20516
Abstract:
Generative AI technologies have been deployed in many places, such as(multimodal) large language models and vision generative models. Theirremarkable performance should be attributed to massive training data andemergent reasoning abilities. …
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Generative AI technologies have been deployed in many places, such as(multimodal) large language models and vision generative models. Theirremarkable performance should be attributed to massive training data andemergent reasoning abilities. However, the models would memorize and generatesensitive, biased, or dangerous information originated from the training dataespecially those from web crawl. New machine unlearning (MU) techniques arebeing developed to reduce or eliminate undesirable knowledge and its effectsfrom the models, because those that were designed for traditionalclassification tasks could not be applied for Generative AI. We offer acomprehensive survey on many things about MU in Generative AI, such as a newproblem formulation, evaluation methods, and a structured discussion on theadvantages and limitations of different kinds of MU techniques. It alsopresents several critical challenges and promising directions in MU research. Acurated list of readings can be found:https://github.com/franciscoliu/GenAI-MU-Reading.
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10.
李翛然
(2025-04-30 10:14):
#paper Computational design of serine hydrolases doi:doi/10.1126/science.adu2454 baker今年的大文章,这周我精读了一下。 哈哈 接了一个活,和这个非常类似,不过比这个难。 需要化学+酶进化+新的工业级纯化方法。 非常非常好。 baker我觉得自从或诺奖后,全面开挂,一定要证明AI在设计结构生物学上不可撼动的作用,绝不是,生物或者化学专家说的,我也能干~~~哈哈哈 这篇文章写的非常好,逻辑非常舒服
Science,
2025-4-18.
DOI: 10.1126/science.adu2454
Abstract:
The design of enzymes with complex active sites that mediate multistep reactions remains an outstanding challenge. With serine hydrolases as a model system, we combined the generative capabilities of RFdiffusion …
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The design of enzymes with complex active sites that mediate multistep reactions remains an outstanding challenge. With serine hydrolases as a model system, we combined the generative capabilities of RFdiffusion with an ensemble generation method for assessing active site preorganization at each step in the reaction to design enzymes starting from minimal active site descriptions. Experimental characterization revealed catalytic efficiencies ( k cat / K m ) up to 2.2 × 10 5 M −1 s −1 and crystal structures that closely match the design models (Cα root mean square deviations <1 angstrom). Selection for structural compatibility across the reaction coordinate enabled identification of new catalysts remove with five different folds distinct from those of natural serine hydrolases. Our de novo approach provides insight into the geometric basis of catalysis and a roadmap for designing enzymes that catalyze multistep transformations.
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11.
颜林林
(2025-04-29 10:22):
#paper doi:10.1101/2025.04.27.25326490, medRxiv, 2025, GWAS of macro-scale resting state functional brain networks identify shared biology with brain structure and autism. 这篇文章对来自UK Biobank的54,030名个体的宏观静息态功能脑网络进行全基因组关联研究(GWAS),首先通过rs-fMRI构建功能脑网络,然后用图论方法总结出涵盖全球、半球和区域水平及其不对称性的多种指标,用这些指标结合SNP位点来做关联分析,找到七个显著的遗传位点和十个候选基因。此后,结合使用已发表的单细胞数据,把细胞类型特异表达与基因突变进行关联,并通过孟德尔随机化证明脑结构与神经表型的关系。然而,在针对12种神经疾病的分析中,仅发现孤独症与找到的图论指标存在显著关联。这篇文章综合了常见的各类方法,除了使用的人群规模较大外,并无特别创新之处,但作为方法运用案例进行学习还是不错的。
medRxiv,
2025-4-28.
DOI: 10.1101/2025.04.27.25326490
Abstract:
The topology of the functional brain network has been associated with several neuropsychiatric conditions. However, little is known about its genetic underpinnings, and whether this overlaps with brain structure and …
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The topology of the functional brain network has been associated with several neuropsychiatric conditions. However, little is known about its genetic underpinnings, and whether this overlaps with brain structure and neuropsychiatric conditions. Hence, we conducted genome-wide association study across six graph metrics of the macroscale functional networks at global, hemispheric and regional levels and their hemispheric asymmetry in 54,030 individuals. We identified seven experiment-wide significant loci and prioritised ten candidate genes. Both global graph metrics and hemispheric asymmetry are modestly heritable, but phenotypically and genetically form two clusters. Furthermore, cortical macro- and microstructure are causally related to global graph metrics and asymmetry, respectively, suggesting a dual structural constraint on functional network organisation. Finally, amongst twelve common neuropsychiatric conditions, only autism was genetically correlated with graph metrics of the functional network, supporting phenotypic case-control differences in functional connectivity. Overall, our results suggest different genetic axes shaping different aspects of brain functional topology and demonstrate shared biology with brain structure and autism.
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12.
燕赵孤侠
(2025-04-28 11:49):
#paper doi: CNKI:SUN:HJGL.0.2012-01-024,2012, 《环境空气质量标准》,环境空气质量标准是守护我们蓝天白云的尺子,也是守护公众健康的核心依据。这篇论文围绕标准的制定背景、技术框架、实施成效及优化路径展开研究,系统梳理了我国空气质量管控的科学逻辑与实践脉络,对大气污染治理的复杂性、科学性与紧迫性有了更深刻的认知。
如何做到科学性,这是需要依托长期监测数据、跨学科所研究出来的成果
如何与经济发展双向奔赴,这需要为未来产业升级、技术进步预留空间,实现既要又要的成果
所面临的挑战,区域差异显著、监测网络覆盖不足、执法效能待提升等
总结,唯有持续优化标准、强化执行、凝聚共识,才能让 “蓝天白云” 成为常态,为美丽中国建设筑牢生态根基。
13.
徐炳祥
(2025-04-28 08:57):
#paper doi: 10.34133/bmr.0171. Biomaterials Research, 2025, A New Perspective on Precision Medicine: The Power of Digital Organoids。这是一篇有关肿瘤类器官研究和应用的综述,作者对肿瘤类器官的特点和现存主要培养方法做了详细介绍。也介绍了单细胞组学、生物信息学、成像技术和人工智能等新兴技术在基于肿瘤类器官的几个重要精准医疗课题,如药物筛选、疗效和副作用评估等,中的应用。在此基础上,作者分析了当前类器官在精准医疗领域内应用和转化面临的主要挑战和瓶颈,并指出多学科合作可能为类器官从实验室走向临床铺平道路。本综述对当前类器官领域做了较全面的介绍,是了解该领域的良好入门材料。
Biomaterials Research,
2025-1.
DOI: 10.34133/bmr.0171
Abstract:
Precision medicine is a personalized medical model based on the individual’s genome, phenotype, and lifestyle that provides tailored treatment plans for patients. In this context, tumor organoids, a 3-dimensional preclinical …
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Precision medicine is a personalized medical model based on the individual’s genome, phenotype, and lifestyle that provides tailored treatment plans for patients. In this context, tumor organoids, a 3-dimensional preclinical model based on patient-derived tumor cell self-organization, combined with digital analysis methods, such as high-throughput sequencing and image processing technology, can be used to analyze the genome, transcriptome, and cellular heterogeneity of tumors, so as to accurately track and assess the growth process, genetic characteristics, and drug responsiveness of tumor organoids, thereby facilitating the implementation of precision medicine. This interdisciplinary approach is expected to promote the innovation of cancer diagnosis and enhance personalized treatment. In this review, the characteristics and culture methods of tumor organoids are summarized, and the application of multi-omics, such as bioinformatics and artificial intelligence, and the digital methods of organoids in precision medicine research are discussed. Finally, this review explores the main causes and potential solutions for the bottleneck in the clinical translation of digital tumor organoids, proposes the prospects of multidisciplinary cooperation and clinical transformation to narrow the gap between laboratory and clinical settings, and provides references for research and development in this field.
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14.
DeDe宝
(2025-04-15 01:15):
#paper doi:https://doi.org/10.1523/JNEUROSCI.1552-24.20254 Prioritizing Working Memory Resources Depends on the Prefrontal Cortex 这篇文章的核心内容是研究前额叶皮层(特别是上外侧前额叶皮层,sPCS)在工作记忆资源分配中的作用。工作记忆是指我们能够短暂存储和操作不再呈现的信息的能力。工作记忆的资源有限,因此我们需要根据行为相关性分配这些资源。一些研究认为前额叶皮层存储工作记忆内容,另一些研究认为它控制工作记忆内容的分配。本研究通过实验区分这两种理论。被试完成了优先级判断的TMS任务。任务中,两个工作记忆项目的优先级通过操纵哪个项目更有可能在记忆延迟后被测试来确定,高优先级项目被测试的可能性是低优先级项目的两倍。参与者首先注视屏幕中心,然后出现一个优先级提示,指示哪个半视野将包含高优先级项目。随后,两个工作记忆项目(小白色点)出现在视野的边缘。经过一个延迟期(2500-3500毫秒)后,一个响应提示出现在注视点,指示参与者进行记忆引导的眼动。其中,TMS在每个试验的延迟期中间以50Hz的频率施加三次脉冲。相比于没有TMS的控制组,TMS刺激sPCS后,低优先级项目的记忆错误显著降低,而高优先级项目的记忆错误没有显著变化。这表明TMS破坏了工作记忆资源的优先级分配,导致低优先级项目的记忆表现意外地提高。此外,TMS对sPCS的影响具有空间特异性,且与对IPS2的刺激效果不同,进一步证实了sPCS在工作记忆资源分配中的独特作用。
The Journal of Neuroscience,
2025-3-12.
DOI: 10.1523/JNEUROSCI.1552-24.2025
Abstract:
How the prefrontal cortex contributes to working memory remains controversial, as theories differ in their emphasis on its role in storing memories versus controlling their content. To adjudicate between these …
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How the prefrontal cortex contributes to working memory remains controversial, as theories differ in their emphasis on its role in storing memories versus controlling their content. To adjudicate between these competing ideas, we tested how perturbations to the human (both sexes) lateral prefrontal cortex impact the storage and control aspects of working memory during a task that requires human subjects to allocate resources to memory items based on their behavioral priority. Our computational model made a strong prediction that disruption of this control process would counterintuitively improve memory for low-priority items. Remarkably, transcranial magnetic stimulation of retinotopically-defined superior precentral sulcus, but not intraparietal sulcus, unbalanced the prioritization of resources, improving memory for low-priority items as predicted by the model. Therefore, these results provide direct causal support for models in which the prefrontal cortex controls the allocation of resources that support working memory, rather than simply storing the features of memoranda.
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15.
惊鸿
(2025-04-14 22:39):
#paper Will AI improve your life? Here’s what 4,000 researchers think Pub Date : 2025-04-09
DOI : 10.1038/d41586-025-01123-x
这是一篇对4260名AI研究员调查AI是否会使人类受益的评价,研究者中54%认为技术利大于弊,仅13%英国公众认同;双方均担忧虚假信息与数据滥用,但科学家更反对企业滥用盗版数据训练模型,呼吁审慎政策。
调查显示,科学家对AI的教育(75%)、医疗(57%)潜力高度乐观,但警惕数据伦理风险:仅25%支持企业使用盗版书籍/论文训练模型,近半数(49%)要求明确用户授权。研究负责人Cian O'Donovan指出,政策需平衡创新与风险,例如建立数据补偿机制,而非简单“选择退出”规则。牛津专家Robert Trager强调,AI安全需结合技术优化与数据伦理,各国政策应纳入“红队测试”等防护机制。
Nature,
2025-4-17.
DOI: 10.1038/d41586-025-01123-x
Abstract:
No abstract available.
16.
刘昊辰
(2025-04-10 15:46):
#paper Ancestor-Based α-β Bounds for Monte-Carlo Tree Search. 这是一篇关于如何通过引入祖先节点的α-β剪枝思想,改进MCTS的探索-利用平衡,提升决策准确性的研究论文。论文动态调整路径上的α(下界)和β(上界)值,结合UCT(Upper Confidence Bound for Trees)的置信区间。 通过公式计算边界差异度量和探索调整项,修改UCT的选择策略。当α<β时优先利用已知路径,当α>β时转向探索新路径,类似极小极大剪枝。下载地址:https://bnaic2024.sites.uu.nl/wp-content/uploads/sites/986/2024/10/Ancestor-Based-%CE%B1-%CE%B2-Bounds-for-Monte-Carlo-Tree-Search.pdf
BNAIC/BeNeLearn 2024,
2024/10.
Abstract:
No abstract available.
17.
龙海晨
(2025-04-09 22:12):
#paper Schiavello E, Biassoni V, Gattuso G, Podda M, Chiaravalli S, Barretta F, Antonelli M, De Cecco L, Pecori E, Gandola L, Massimino M. A homogeneous treatment for non-DIPG diffuse midline glioma. Tumori. 2023 Jun;109(3):269-275. doi: 10.1177/03008916221099067. Epub 2022 Jun 16. PMID: 35708347; PMCID: PMC10248290. 这是一篇研究神经胶质瘤的文章。与弥漫性中线神经胶质瘤(diffuse intrinsic pontine gliomas ,DIPG)类似H3K27M突变中线神经胶质瘤(DMG)的预后情况普遍很差。文章对DMG患者进行放射疗法和伴随辅助治疗尼妥珠单抗(Nimotuzumab)辅助治疗Vinorelbine(长春瑞滨)。治疗了九名患者,其中七个女性,年龄13岁。三名患者进行了活检,六名患者进行了部分肿瘤切除术。局部进展的中位时间为12.7个月,中位总生存期为17.8个月。六名患者死于肿瘤进展,进展时脑出血之一。诊断后的38.6和46.3个月后,有两个还活着,一个是连续缓解的,另一个是在复发后的持续缓解。一年的无进展生存率为33.3%。 1、2和3年的总生存率分别为88.9%,33.3%和22.2%。
Tumori Journal,
2023-6.
DOI: 10.1177/03008916221099067
Abstract:
Introduction: The H3K27M-mutant diffuse midline glioma (DMG) was first included in the World Health Organization (WHO) Classification of central nervous system (CNS) tumors in 2016, and confirmed in its fifth …
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Introduction: The H3K27M-mutant diffuse midline glioma (DMG) was first included in the World Health Organization (WHO) Classification of central nervous system (CNS) tumors in 2016, and confirmed in its fifth edition. The biological behavior and dismal prognosis of this tumor resemble diffuse intrinsic pontine gliomas (DIPG). Homogeneously-treated series are rarely reported. Methods: From 2016 onwards, we treated patients with DMG with radiotherapy and concomitant/adjuvant nimotuzumab/vinorelbine, plus re-irradiation at relapse, as already done for DIPG. Results: We treated nine patients, seven females, with a median age at diagnosis of 13 years. Tumor sites were: thalamic in five cases, pontocerebellar in two, pineal in one, and paratrigonal with nodular/leptomeningeal dissemination in one. Three patients were biopsied, and six had partial tumor resections. Central pathological review was always performed. The median time to local progression was 12.7 months, and the median overall survival was 17.8 months. Six patients died of tumor progression, one of cerebral bleeding at progression. Two were alive, one in continuous remission, the other after relapsing, at 38.6 and 46.3 months after diagnosis. Progression-free survival was 33.3% at one year. Overall survival was 88.9%, 33.3% and 22.2% at 1, 2 and 3 years, respectively. Conclusions: This is a small series of homogeneously-treated DMG patients. The results obtained are comparable with those of DIPG patients. Given the phenotypically- and molecularly-defined setting of DMG and severe outcome in this orphan population, they should be treated and included in registries and protocols of DIPG.
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18.
翁凯
(2025-04-05 09:05):
#paper 【doi】10.1038/s41586-025-08732-6;【发表年份】2025年;【期刊】Nature;【标题】VDAC2 loss elicits tumour destruction and inflammation for cancer therapy。本研究发现,电压依赖性阴离子通道2(VDAC2)在肿瘤免疫逃逸中起关键作用。研究者利用CRISPR–Cas9基因筛选技术,发现VDAC2是免疫信号依赖的检查点,限制了干扰素-γ(IFNγ)介导的肿瘤破坏和肿瘤微环境的炎症重编程。研究中,靶向肿瘤细胞中的VDAC2能够增强IFNγ诱导的细胞死亡和cGAS–STING信号通路的激活,显著改善抗肿瘤效果和免疫治疗反应。通过全基因组遗传互作筛选,研究者确定BAK是VDAC2缺失诱导效应的介质。机制上,IFNγ刺激增加了BIM、BID和BAK的表达,而VDAC2的缺失则导致IFNγ诱导的BAK激活失控,进而引发线粒体损伤。随后,线粒体DNA异常释放到细胞质中,触发了cGAS–STING信号通路和I型干扰素反应的强烈激活。重要的是,共同缺失STING信号通路组分会削弱肿瘤细胞中VDAC2耗竭的治疗效果,这表明靶向VDAC2整合了CD8+ T细胞和IFNγ介导的适应性免疫与肿瘤内源性类固有免疫反应。研究结果揭示了VDAC2作为一个双重作用靶点,能够克服肿瘤免疫逃逸,并强调了协同破坏和炎症化肿瘤以实现有效癌症免疫治疗的重要性。
19.
翁凯
(2025-04-03 20:39):
#paper 【doi】10.1016/j.cell.2025.03.018;【发表年份】2025年;【期刊】Cell;【标题】A single-cell atlas reveals immune heterogeneity in anti-PD-1-treated non-small cell lung cancer。本研究的目标是探究非小细胞肺癌(NSCLC)患者在接受抗PD-1治疗后的免疫微环境异质性,以了解不同患者对治疗反应的差异。研究者们通过单细胞RNA测序(scRNA-seq)和T细胞受体测序(scTCR-seq)分析了234名接受新辅助化疗联合抗PD-1治疗的NSCLC患者的肿瘤样本。研究发现,这些患者可以分为五种不同的肿瘤免疫微环境(TIME)亚型,这些亚型与主要病理反应(MPR)率相关。具体来说,NK细胞、记忆B细胞和耗竭前体T细胞与MPR相关,而非MPR患者则表现出更高的CCR8+ Tregs水平。此外,T细胞克隆扩增特征分析揭示了非MPR患者中的异质性,表现为Tex相关细胞和CCR8+ Tregs的不同扩增程度。研究还发现,耗竭前体T细胞(Texp细胞)的比例与无复发生存期相关,能够识别出尽管未达到MPR但复发风险降低的患者亚组。这项研究提供了对化疗免疫治疗反应的TIME异质性分析,为NSCLC管理提供了新的见解。
Abstract:
Anti-PD-(L)1 treatment is standard for non-small cell lung cancer (NSCLC), but patients show variable responses to the same regimen. The tumor immune microenvironment (TIME) is associated with immunotherapy response, yet …
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Anti-PD-(L)1 treatment is standard for non-small cell lung cancer (NSCLC), but patients show variable responses to the same regimen. The tumor immune microenvironment (TIME) is associated with immunotherapy response, yet the heterogeneous underlying therapeutic outcomes remain underexplored. We applied single-cell RNA and TCR sequencing (scRNA/TCR-seq) to analyze surgical tumor samples from 234 NSCLC patients post-neoadjuvant chemo-immunotherapy. Analyses revealed five distinct TIME subtypes with varying major pathological response (MPR) rates. MPR patients had elevated levels of FGFBP2 NK/NK-like T cells, memory B cells, or effector T cells, while non-MPR patients showed higher CCR8 Tregs. T cell clonal expansion analyses unveiled heterogeneity in non-MPR patients, marked by varying expansions of Tex-relevant cells and CCR8 Tregs. Precursor exhausted T cells (Texp cells) correlated with recurrence-free survival, identifying a patient subgroup with reduced recurrence risk despite lack of MPR. Our study dissects TIME heterogeneity in response to chemoimmunotherapy, offering insights for NSCLC management.
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