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1.
龙海晨 (2024-07-08 09:45):
#paper Li X, Sun X, Li L, Luo Y, Chi Y, Zheng G. MDM2-mediated ubiquitination of LKB1 contributes to the development of diabetic cataract. Exp Cell Res. 2022 Aug 1;417(1):113191. doi: 10.1016/j.yexcr.2022.113191. Epub 2022 May 2. PMID: 35513074.糖尿病性白内障(Diabetic cataract,DC)是糖尿病的常见并发症。晶状体上皮细胞(lens epithelial cells , LEC)的上皮-间质转化(epithelial-mesenchymal transition, EMT)在DC发展中起关键作用。鼠双微体2(Murine double minute 2 ,MDM2)是一种E3泛素连接酶,通过调节多种靶点促进EMT。文章发现DC患者和大鼠晶状体中MDM2的mRNA和蛋白质水平上调。因此,构建了高糖(high glucose ,HG)诱导的人晶状体上皮细胞( human lens epithelial cells,HLEC)进行进一步研究。结果表明,HG培养的HLEC中MDM2水平升高,MDM2敲低减轻了HG诱导的异常迁移、EMT和氧化应激损伤。免疫共沉淀和泛素化试验表明,MDM2 通过泛素化降解下调了 LKB1 的表达。研究发现,LKB1 在人类和大鼠 DC 晶状体以及 HG 刺激的 HLEC 中表达较低。此外,LKB1 过表达减轻了 HG 诱导的 HLEC 功能障碍。研究数据显示,MDM2 敲低引起的 EMT 和氧化应激相关变化可以通过 LKB1 下调得到恢复。
2.
龙海晨 (2024-06-06 01:44):
#paper Sun Q, Zhang Y, Wang S, Yang F, Cai H, Xing Y, Zhou L, Chen S, Wang Y. LncRNA HOTAIR promotes α-synuclein aggregation and apoptosis of SH-SY5Y cells by regulating miR-221-3p in Parkinson's disease. Exp Cell Res. 2022 Aug 1;417(1):113132. doi: 10.1016/j.yexcr.2022.113132. Epub 2022 Apr 6. PMID: 35398161. 帕金森病 (Parkinson's disease,PD) 是一种常见的神经退行性疾病,其特征是神经元逐渐丢失。PD 的发病机制与细胞凋亡、炎症、氧化应激和 α-突触核蛋白聚集体的积累密切相关。本研究旨在探讨长链非编码RNA(long non-coding RNA ,lncRNA)HOX转录本反义RNA(HOX transcript antisense RNA,HOTAIR)在PD中的作用及其机制,文章研究检测了1-甲基-4-苯基-1,2,3,6-四氢吡啶盐酸盐(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-hydrochloride,MPTP)处理的小鼠脑组织中HOTAIR、miR-221-3p和α-突触核蛋白的表达水平,探讨了HOTAIR对MPP +处理的SH-SY5Y细胞活力、凋亡、炎症和氧化应激的影响,并研究了HOTAIR/miR-221-3p/α-突触核蛋白的ceRNA调控网络。文章的研究结果显示,1、在 PD 模型中,HOTAIR 表达水平较高,而 miR-221-3p 表达水平较低。2、HOTAIR 敲低降低了 MPP+ 的神经毒性。3、HOTAIR降低了miR-221-3p的表达。4、α-突触核蛋白是 miR-221-3p 的靶基因。5、抑制 miR-221-3p 可逆转HOTAIR 敲低的神经保护作用。
Abstract:
Parkinson's disease (PD) is a common neurodegenerative disease. Here, the purpose of the study was to explore the function of long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) in … >>>
Parkinson's disease (PD) is a common neurodegenerative disease. Here, the purpose of the study was to explore the function of long non-coding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) in PD and its underlying mechanism. An in vivo 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-hydrochloride (MPTP)-induced mouse model of PD was generated and the SH-SY5Y cells were treated with MPP  to induce neuronal damage in vitro. Quantitative real-time polymerase chain reaction (QRT-PCR) and Western blot were used to detect the expression of HOTAIR, miR-221-3p, α-synuclein and apoptosis-related genes. MTT, flow cytometry and TUNEL assay was used to detect cell viability and apoptosis, respectively. The levels of inflammatory cytokines TNF-α,IL-1β and IL-6 were detected by ELISA assay. The levels of lactate dehydrogenase (LDH), reactive oxygen species (ROS), and superoxide dismutase (SOD) were determined using the appropriate assay kits. The interactions between miR-221-3p and HOTAIR or α-synuclein were determined by dual luciferase assay and RNA binding protein immunoprecipitation (RIP). Co-localization of HOTAIR and miR-221-3p was also proved by immunofluorescence staining. The results showed that HOTAIR was highly expressed, while miR-221-3p expression was decreased in PD model in vivo and in vitro. In SH-SY5Y cells treated with MPP, the knockdown of HOTAIR increased cell viability and reduced cell apoptosis, the secretion of inflammatory cytokines and oxidative stress reaction, while HOTAIR overexpression led to opposite effects. Furthermore, HOTAIR sponged miR-221-3p which directly targeted α-synuclein and thus regulated the expression of α-synuclein. Meanwhile, inhibiting miR-221-3p could partially reverse the neuroprotective effects of HOTAIR knockdown. In conclusion, HOTAIR attenuated the injury of SH-SY5Y cells induced by MPP via miR-221-3p/α-synuclein axis, suggesting the potential therapeutic value of HOTAIR in PD. <<<
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3.
龙海晨 (2024-05-06 19:00):
#paper Wei X, Wu J, Li J, Yang Q. PLK2 targets GSK3β to protect against cisplatin-induced acute kidney injury. Exp Cell Res. 2022 Aug 1;417(1):113181. doi: 10.1016/j.yexcr.2022.113181. Epub 2022 May 4. PMID: 35523306.顺铂引起的急性肾损伤(AKI)是一种复杂的危重疾病,伴有肾功能迅速下降和高死亡风险,目前尚无有效或特异的治疗方法。Polo 样激酶 2 Polo-like kinase 2(PLK2) 是一种丝氨酸/苏氨酸激酶,参与多种疾病的进展,包括癌症、心脏纤维化、糖尿病肾病等。文章鉴定出 PLK2 是 AKI 肾组织中显着上调的基因,然后在不同的 AKI 动物模型和细胞模型中进行了验证。使用 siRNA 或抑制剂抑制 PLK2 可以通过诱导严重的细胞凋亡和氧化应激损伤来增强顺铂诱导的 AKI,而强制 PLK2 表达可以预防顺铂诱导的肾功能障碍。文章的主要亮点:PLK2 在急性肾损伤模型中显着增强。上调 PLK2 可以逆转顺铂诱导的细胞凋亡和氧化应激。PLK2 通过磷酸化 GSK3β 来调节顺铂诱导的细胞凋亡和氧化应激。
Abstract:
Cisplatin-induced acute kidney injury (AKI), which is accompanied by a rapid decline in renal function and a high risk of death, is a complex critical illness with no effective or … >>>
Cisplatin-induced acute kidney injury (AKI), which is accompanied by a rapid decline in renal function and a high risk of death, is a complex critical illness with no effective or specific treatment. Polo-like kinase 2 (PLK2), a serine/threonine kinase, is involved in the progression of multiple diseases, including cancers, cardiac fibrosis, diabetic nephropathy, etc. Here, by integrating two Gene Expression Omnibus (GEO) datasets of cisplatin-induced AKI animal models, we identified PLK2 as a significantly up-regulated gene in AKI renal tissues, which was then verified in different AKI animal models and cell models. Suppressing PLK2 using siRNAs or inhibitors could enhance cisplatin-induced AKI by inducing severe apoptosis and oxidative stress damage, while enforced PLK2 expression could prevent renal dysfunction induced by cisplatin. We further discovered that PLK2 might phosphorylate glycogen synthase kinase 3β (GSK3β) in the pathogenesis of AKI. In conclusion, our results show that PLK2 play a protective role in cisplatin-induced AKI and may be a new protective target of cisplatin nephrotoxicity. <<<
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4.
龙海晨 (2024-04-01 09:07):
#paper Yamashita S, Tanaka M, Ida C, Kouyama K, Nakae S, Matsuki T, Tsuda M, Shirai T, Kamemura K, Nishi Y, Moss J, Miwa M. Physiological levels of poly(ADP-ribose) during the cell cycle regulate HeLa cell proliferation. Exp Cell Res. 2022 Aug 1;417(1):113163. doi: 10.1016/j.yexcr.2022.113163. Epub 2022 Apr 18. PMID: 35447104; PMCID: PMC10009817. PAR: Poly(ADP-ribose) ;PARG Poly(ADP-ribose) glycohydrolase ; PARP Poly(ADP-ribose) polymerase ;该文章在在不使用PARG抑制剂的情况下量化了HeLa细胞整个细胞周期内PAR的基本水平,发现S期的PAR水平显着高于G1期,其次非应激条件下PAR的半衰期小于40秒,这与S期大量NAD +的消耗一致,这是由PARP抑制剂挽救的。第三,PARP抑制剂使细胞周期延迟至S期。第四,S期期间PAR水平的增加与PARP1、PARG、NAD +和pERK2的水平无关。第五,纯化的 PARP1 在 DNA 不存在的情况下被组蛋白 H4 激活。PARP 抑制剂延迟 S 期细胞周期并减少细胞增殖。
Abstract:
Protein targets of polyADP-ribosylation undergo covalent modification with high-molecular-weight, branched poly(ADP-ribose) (PAR) of lengths up to 200 or more ADP-ribose residues derived from NAD. PAR polymerase 1 (PARP1) is the … >>>
Protein targets of polyADP-ribosylation undergo covalent modification with high-molecular-weight, branched poly(ADP-ribose) (PAR) of lengths up to 200 or more ADP-ribose residues derived from NAD. PAR polymerase 1 (PARP1) is the most abundant and well-characterized enzyme involved in PAR biosynthesis. Extensive studies have been carried out to determine how polyADP-ribosylation (PARylation) regulates cell proliferation during cell cycle, with conflicting conclusions. Since significant activation of PARP1 occurs during cell lysis in vitro, we changed the standard method for cell lysis, and using our sensitive ELISA system, quantified without addition of a PAR glycohydrolase inhibitor and clarified that the PAR level is significantly higher in S phase than that in G1. Under normal condition in the absence of exogenous DNA-damaging agent, PAR turns over with a half-life of <40 s; consistent with significant decrease of NAD levels in S phase, which is rescued by PARP inhibitors, in line with the observed rapid turnover of PAR. PARP inhibitors delayed cell cycle in S phase and decreased cell proliferation. Our results underscore the importance of a suitable assay system to measure rapid PAR chain dynamics in living cells and aid our understanding of the function of PARylation during the cell cycle. <<<
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5.
龙海晨 (2024-03-08 21:11):
#paper Catta-Preta CMC, de Azevedo-Martins AC, de Souza W, Motta MCM. Effect of the endoplasmic reticulum stressor tunicamycin in Angomonas deanei heat-shock protein expression and on the association with the endosymbiotic bacterium. Exp Cell Res. 2022 Aug 1;417(1):113162. doi: 10.1016/j.yexcr.2022.113162. Epub 2022 Apr 20. PMID: 35460679.这是一篇研究细胞基础结构方面的文章,文章用锥虫细胞为对象研究内质网(ER)应激。用衣霉素Tunicamycin (TM) 作为诱导剂,研究热休克蛋白Hsp90 表达变化。通过超微结构和蛋白质组学方法研究了TM在单胞菌及其共生细菌之间的关联中的作用。所获得的数据表明了ER对原核生物和真核生物之间共生关系的适应和维持的重要性。
Abstract:
The endoplasmic reticulum (ER) presents unique properties to establishing bacterium symbiosis in eukaryotic cells since it synthesizes and glycosylates essential molecules like proteins and lipids. Tunicamycin (TM) is an antibiotic … >>>
The endoplasmic reticulum (ER) presents unique properties to establishing bacterium symbiosis in eukaryotic cells since it synthesizes and glycosylates essential molecules like proteins and lipids. Tunicamycin (TM) is an antibiotic that inhibits the first step in the N-linked glycosylation in eukaryotes and has been used as an ER stress inducer to activate the Unfolded Protein Response (UPR). Mutualistic symbiosis in trypanosomatids is characterized by structural adaptations and intense metabolic exchanges, thus we investigated the effects of TM in the association between Angomonas deanei and its symbiotic bacterium, through ultrastructural and proteomic approaches. Cells treated with the inhibitor showed a decrease in proliferation, enlargement of the ER and Golgi cisternae and an increased distance between the symbiont and the ER. TM proved to be an important tool to better understand ER stress in trypanosomatids, since changes in protein composition were observed in the host protozoan, especially the expression of the Hsp90 chaperone. Furthermore, data obtained indicates the importance of the ER for the adaptation and maintenance of symbiotic associations between prokaryotes and eukaryotes, considering that this organelle has recognized importance in the biogenesis and division of cell structures. <<<
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6.
龙海晨 (2024-02-09 19:34):
#paper Ahmed Mostafa G, Mohamed Ibrahim H, Al Sayed Shehab A, Mohamed Magdy S, AboAbdoun Soliman N, Fathy El-Sherif D. Up-regulated serum levels of interleukin (IL)-17A and IL-22 in Egyptian pediatric patients with COVID-19 and MIS-C: Relation to the disease outcome. Cytokine. 2022 Jun;154:155870. doi: 10.1016/j.cyto.2022.155870. Epub 2022 Apr 4. PMID: 35398721; PMCID: PMC8977483.这是一篇研究新冠感染儿童血清中各种细胞因子与新冠严重程度的文章。检测患者40名(其中 18 名患者患有 COVID-19,22 名患者患有多系统炎症综合征“MIS-C”)年龄从两个月到16周岁,48名健康儿童作为对照组。COVID-19 和 MIS-C 患者的血清 IL-17A 和 IL-22 水平显着高于健康对照儿童。患者的血清 IL-17A 和 IL-22 水平均升高。90% 的患者发现 CRP 和血清铁蛋白水平升高。IL-17和IL-22都被认为是组织信号细胞因子,有利于肺、皮肤和胃肠道等上皮屏障器官的保护和再生。IL-17 和 IL-22 在宿主抵御微生物以及急性和慢性炎症性疾病的发展中发挥着重要作用。但康复的患者与死亡的患者生前留存的血清中对比 IL-17A、IL-22 没有显著差异, IL-17A、IL-22 的升高与否不能作为预后指标。
Abstract:
Both IL-17A and IL-22 share cellular sources and signaling pathways. They have synergistic action on epithelial cells to stimulate their production of antimicrobial peptides which are protective against infections. However, … >>>
Both IL-17A and IL-22 share cellular sources and signaling pathways. They have synergistic action on epithelial cells to stimulate their production of antimicrobial peptides which are protective against infections. However, both interleukins may contribute to ARDS pathology if their production is not controlled. This study aimed to investigate serum levels of IL-17A and IL-22 in relation to the disease outcome in patients with SARS-CoV-2. Serum IL-17A and IL-22 were measured by ELISA in 40 patients with SARS-CoV-2, aged between 2 months and 16 years, (18 had COVID-19 and 22 had multisystem inflammatory syndrome in children "MIS-C") in comparison to 48 age- and sex-matched healthy control children. Patients with COVID-19 and MIS-C had significantly higher serum IL-17A and IL-22 levels than healthy control children (P < 0.001). Increased serum IL-17A and IL-22 levels were found in all patients. Elevated CRP and serum ferritin levels were found in 90% of these patients. Lymphopenia, neutrophilia, neutropenia, thrombocytopenia and elevated ALT, LDH and D-dimer were found in 45%, 42.5 %, 2.5%, 30%, 32.5%, 82.5%, and 65%, respectively of these patients. There were non-significant differences between patients who recovered and those who died or had a residual illness in serum levels of IL-17A, IL-22 and the routine inflammatory markers of COVID-19. In conclusions, serum IL-17A and IL-22 levels were up-regulated in all patients with COVID-19 and MIS-C. Levels of serum IL-17A, IL-22 and the routine inflammatory markers of COVID-19 were not correlated with the disease outcome. Our conclusions are limited by the sample size. <<<
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7.
龙海晨 (2024-01-23 11:39):
#paper Matarazzo L, Hernandez Santana YE, Walsh PT, Fallon PG. The IL-1 cytokine family as custodians of barrier immunity. Cytokine. 2022 Jun;154:155890. doi: 10.1016/j.cyto.2022.155890. Epub 2022 Apr 21. PMID: 35462264.这是一篇介绍白介素1(IL-1)的综述,文章介绍了白介素家族成员的性质,人体相关的器官与白介素的关系,白介素相关的疾病,包括肺部,皮肤,肠道。不同的白介素在人体不同疾病中的作用。如何发挥免疫作用。
Abstract:
The interleukin-1 (IL-1) family of cytokines and receptors are implicated in the functioning of innate and adaptive immunity and the genesis of inflammation. They are widely expressed in structural and … >>>
The interleukin-1 (IL-1) family of cytokines and receptors are implicated in the functioning of innate and adaptive immunity and the genesis of inflammation. They are widely expressed in structural and immune cells with marked expression within barrier mucosal surfaces. In the lung, gut and skin, which are common entry sites for pathogens, they play essential functions in maintaining the functional integrity of the barrier and manage innate and adaptive immunity in response to insult and infections. In tissue sites, the IL-1 cytokines are tightly regulated by mechanisms involving decoy receptors and protease degradation. Dysregulation of these processes are associated with aberrant tissue inflammation leading to a number of inflammatory diseases. This review will address the roles of the different IL-1 cytokines at the lung, gut and skin barrier surfaces at homeostasis, and their roles as inflammatory mediators in diseases such as asthma, chronic obstructive pulmonary disease, inflammatory bowel diseases, atopic dermatitis and psoriasis. <<<
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8.
龙海晨 (2023-12-31 20:01):
#paper Zhang W, Ma Z, Wu Y, Shi X, Zhang Y, Zhang M, Zhang M, Wang L, Liu W. SARS-CoV-2 3C-like protease antagonizes interferon-beta production by facilitating the degradation of IRF3. Cytokine. 2021 Dec;148:155697. doi: 10.1016/j.cyto.2021.155697. Epub 2021 Sep 3. PMID: 34509038; PMCID: PMC8413301. 文章通过对转染SARS-CoV-2的293T细胞(人)。研究感染病毒后,细胞的 RLR 通路,发现感染后,I 型干扰素的产生受到 SARS-CoV-2 3CL的显著影响。
Abstract:
The prevalence of SARS-CoV-2 is a great threat to global public health. However, the relationship between the viral pathogen SARS-CoV-2 and host innate immunity has not yet been well studied. … >>>
The prevalence of SARS-CoV-2 is a great threat to global public health. However, the relationship between the viral pathogen SARS-CoV-2 and host innate immunity has not yet been well studied. The genome of SARS-CoV-2 encodes a viral protease called 3C-like protease. This protease is responsible for cleaving viral polyproteins during replication. In this investigation, 293T cells were transfected with SARS-CoV-2 3CL and then infected with Sendai virus (SeV) to induce the RIG-I like receptor (RLR)-based immune pathway. q-PCR, luciferase reporter assays, and western blotting were used for experimental analyses. We found that SARS-CoV-2 3CL significantly downregulated IFN-β mRNA levels. Upon SeV infection, SARS-CoV-2 3CL inhibited the nuclear translocation of IRF3 and p65 and promoted the degradation of IRF3. This effect of SARS-CoV-2 3CL on type I IFN in the RLR immune pathway opens up novel ideas for future research on SARS-CoV-2. <<<
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9.
龙海晨 (2023-11-30 01:51):
#paper Janbazacyabar H, van Bergenhenegouwen J, Varasteh S, Garssen J, Folkerts G, Braber S. Repeated exposure of bronchial epithelial cells to particular matter increases allergen-induced cytokine release and permeability. Cytokine. 2022 Jun;154:155878. doi: 10.1016/j.cyto.2022.155878. Epub 2022 Apr 8. PMID: 35405483. 这是一篇研究暴露在颗粒污染物下与过敏的文章。作者构建了一个通有电极的细胞模型。用以模拟检测长期暴露在柴油废气颗粒物环境下细胞的过敏反应。为进一步研究呼吸道在重污染下的过敏,哮喘提供了基础。
Abstract:
Long term particulate matter (PM) exposure has been associated with an increased incidence of respiratory diseases. Here, an in vitro model was developed to study how long term diesel exhaust … >>>
Long term particulate matter (PM) exposure has been associated with an increased incidence of respiratory diseases. Here, an in vitro model was developed to study how long term diesel exhaust particle (DEP) exposure might predispose to the development of allergic reactions. Airway epithelial (16HBE) cells were exposed to low concentrations of diesel exhaust particle (DEP) for 4 days after which they were challenged with house dust mite (HDM) extract (24 h). Compared to acute exposure (24 h), 4 days DEP exposure to 16HBE cells further reduced the transepithelial electrical resistance (TEER) and increased CXCL-8 release. DEP pre-exposure aggravated HDM-induced loss of TEER, increased tracer flux across the barrier and reduced CLDN-3 expression in these 16HBE cells. HDM-induced cytokine (IL-6, CCL-22, IL-10 and CXCL-8) release was significantly increased after DEP pre-exposure. In the current study an in vitro model with long term PM exposure was presented, which might be helpful for further understanding the interplay between long term PM exposure and allergic responses. <<<
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10.
龙海晨 (2023-10-25 12:35):
#paper Chaves DG, de Oliveira LC, da Silva Malta MCF, de Oliveira IR, Barbosa-Stancioli EF, Teixeira MM, Martins ML. Pro-inflammatory immune profile mediated by TNF and IFN-γ and regulated by IL-10 is associated to IgG anti-SARS-CoV-2 in asymptomatic blood donors. Cytokine. 2022 Jun;154:155874. doi: 10.1016/j.cyto.2022.155874. Epub 2022 Apr 4. PMID: 35397248; PMCID: PMC8977499. 文章是巴西在新冠时期做的研究,对7837名献血者做的研究检测血液中的抗体水平。发现症状严重的TNF、IFN-γ和IL-10浓度较高。症状的严重程度与IgG抗体的中和能力有关。而IgG抗体的中和能力和血清的CCL5和CXCL10显著相关。文章据此假设维持在IL-10调节良好的促炎微环境中可以提高对新冠的免疫适应性。
Abstract:
The SARS-CoV-2 virus has infected and killed millions of people, but little is known about the risk factors that lead to the development of severe, mild or asymptomatic conditions after … >>>
The SARS-CoV-2 virus has infected and killed millions of people, but little is known about the risk factors that lead to the development of severe, mild or asymptomatic conditions after infection. The individual immune response and the balance of cytokines and chemokines have been shown to be important for the prognosis of patients. Additionally, it is essential to understand how the production of specific antibodies with viral neutralizing capacity is established. In this context, this study aimed to identify positive individuals for IgG anti-SARS-CoV-2 in a large population of blood donors (n = 7837) to establish their immune response profile and to evaluate its viral neutralization capacity. The prevalence found for IgG anti-SARS-CoV-2 was 5.6% (n = 441), with male blood donors (61.9%) being more prevalent among the positive ones. The results showed that positive individuals for IgG anti-SARS-CoV-2 have high serum concentrations of chemokines, TNF, IFN-γ and IL-10. The analyses showed that the positivity index for IgG anti-SARS-CoV-2 is associated with the neutralizing capacity of the antibodies, which, in turn, is significantly related to lower serum concentrations of CCL5 and CXCL10. The results allow us to hypothesize that the development and maintenance of IgG anti-SARS-CoV-2 antibodies in infected individuals occurs in a pro-inflammatory microenvironment well regulated by IL-10 with great capacity for recruiting cells from the innate and adaptive immune systems. <<<
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11.
龙海晨 (2023-09-27 10:40):
#paper Falahi S, Zamanian MH, Feizollahi P, Rezaiemanesh A, Salari F, Mahmoudi Z, Gorgin Karaji A. Evaluation of the relationship between IL-6 gene single nucleotide polymorphisms and the severity of COVID-19 in an Iranian population. Cytokine. 2022 Jun;154:155889. doi: 10.1016/j.cyto.2022.155889. Epub 2022 Apr 19. PMID: 35461173; PMCID: PMC9015956. 这是一篇2022年发表的新冠研究的文章。这个杂志影响因子3.8,对于这个杂志我真是感情复杂,当初我2022年投稿该杂志,他们说我不适合在他们这发表,然后给我推荐了另一个杂志,是杂志内部推荐,我的文章自动过去,然后他推荐的那个杂志把我拒稿了。现在,我很感谢他们当初的不发之恩,最后我的文章在4.6的杂志上发表还是中科院的Top期刊。这是一篇阴性结果的研究,估计就因为蹭了当时新冠的热点得以发表。做的实验不多研究的也简单,就是SNP位点的,研究的伊朗克尔曼沙赫库尔德人,共346人(175名重度新冠肺炎患者和171名轻度新冠肺炎患者)分析白细胞介素-6(IL-6)基因多态性,从患者外周血白细胞中提取基因组DNA以确定三个选择的SNPs的基因型(rs1800795(−174 G>C)、rs1800796(−572 G>C,和rs1800797(−597 G>A)),结果表明这些SNP与伊朗克尔曼沙赫库尔德人口中新冠肺炎的严重程度无关。SNP,阴性结果,如果不是当初新冠的热度,估计发不了这么高分的杂志。
Abstract:
BACKGROUND: Emerged coronavirus disease 2019 (COVID-19) is a pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2). Disease severity is associated with elevated levels of proinflammatory cytokines, such … >>>
BACKGROUND: Emerged coronavirus disease 2019 (COVID-19) is a pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2). Disease severity is associated with elevated levels of proinflammatory cytokines, such as interleukin-6 (IL-6). Genetic polymorphisms in the regulatory regions of cytokine genes may be associated with differential cytokine production in COVID-19 patients. This study aimed to investigate the association between three potentially functional single-nucleotide polymorphisms (SNPs) in the promoter region of IL-6 and the severity of susceptibility to COVID-19 in an Iranian population. METHODS: In total, 346 individuals (175 patients with severe COVID-19 and 171 patients with mild COVID-19) were recruited for this cohort study. Genomic DNA was extracted from peripheral blood leukocytes of patients to determine the genotypes of three selected SNPs (rs1800795 (-174 G > C), rs1800796 (-572 G > C), and rs1800797 (-597 G > A)) in the promoter region of the IL-6 gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. RESULTS: There were no significant differences in the genotype or allele distribution of selected SNPs (rs1800795 (-174 G > C), rs1800796 (-572 G > C), and rs1800797 (-597 G > A)) in the promoter region of the IL-6 gene in patients with severe COVID-19 and patients with mild COVID-19. DISCUSSION: Our study indicated that these SNPs are not associated with COVID-19 severity in the Kurdish population from Kermanshah, Iran. <<<
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12.
龙海晨 (2023-08-24 13:14):
#paper Fairley JA, Cheetham MH, Patton SJ, Rouleau E, Denis M, Dequeker EMC, Schuuring E, van Casteren K, Fenizia F, Normanno N, Deans ZC. Results of a worldwide external quality assessment of cfDNA testing in lung Cancer. BMC Cancer. 2022 Jul 12;22(1):759. doi: 10.1186/s12885-022-09849-x. PMID: 35820813; PMCID: PMC9275131. 文章是对世界范围内用cfDNA检测肺癌的进行质量评估。45个国家304家注册实验室中有264个实验室提交了结果。排除无法从人造血浆中提取DNA的实验室,大多数实验室获得了准确的结果,对于不同的基因突变采用不同的检测手段准确率不同。实验证明了CFDNA检测肺癌的可行性。
Abstract:
BACKGROUND: Circulating cell free DNA (cfDNA) testing of plasma for EGFR somatic variants in lung cancer patients is being widely implemented and with any new service, external quality assessment (EQA) … >>>
BACKGROUND: Circulating cell free DNA (cfDNA) testing of plasma for EGFR somatic variants in lung cancer patients is being widely implemented and with any new service, external quality assessment (EQA) is required to ensure patient safety. An international consortium, International Quality Network for Pathology (IQNPath), has delivered a second round of assessment to measure the accuracy of cfDNA testing for lung cancer and the interpretation of the results. METHODS: A collaboration of five EQA provider organisations, all members of IQNPath, have delivered the assessment during 2018-19 to a total of 264 laboratories from 45 countries. Bespoke plasma reference material containing a range of EGFR mutations at varying allelic frequencies were supplied to laboratories for testing and reporting according to routine procedures. The genotyping accuracy and clinical reporting was reviewed against standardised criteria and feedback was provided to participants. RESULTS: The overall genotyping error rate in the EQA was found to be 11.1%. Low allelic frequency samples were the most challenging and were not detected by some testing methods, resulting in critical genotyping errors. This was reflected in higher false negative rates for samples with variant allele frequencies (VAF) rates less than 1.5% compared to higher frequencies. A sample with two different EGFR mutations gave inconsistent detection of both mutations. However, for one sample, where two variants were present at a VAF of less than 1% then both mutations were correctly detected in 145/263 laboratories. Reports often did not address the risk that tumour DNA may have not been tested and limitations of the methodologies provided by participants were insufficient. This was reflected in the average interpretation score for the EQA being 1.49 out of a maximum of 2. CONCLUSIONS: The variability in the standard of genotyping and reporting highlighted the need for EQA and educational guidance in this field to ensure the delivery of high-quality clinical services where testing of cfDNA is the only option for clinical management. <<<
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13.
龙海晨 (2023-07-11 02:13):
#paper Sun M, Ji H, Xu N, Jiang P, Qu T, Li Y. Real-world data analysis of immune checkpoint inhibitors in stage III-IV adenocarcinoma and squamous cell carcinoma. BMC Cancer. 2022 Jul 13;22(1):762. doi: 10.1186/s12885-022-09843-3. PMID: 35831785; PMCID: PMC9277844. 这是一篇临床上回顾性研究肺癌的文章,主要研究对象:III-IV期肺腺癌和鳞状细胞癌患者。使用免疫检查点抑制剂immune checkpoint inhibitors (ICIs)方法治疗后的情况。对315名患者使用ICIs疗法,并对患者进行评估,发现,ICIs对肺癌患者有良好的疗效,显著提高了患者生存期中位数,也缓解了症状。从结果数据看,使用ICIs也存在不良反应。
Abstract:
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>This study was designed to investigate the clinical application, efficacy, and safety of immune checkpoint inhibitors (ICIs) in the treatment of lung cancer in the real world.</jats:p> </jats:sec><jats:sec> … >>>
<jats:title>Abstract</jats:title><jats:sec> <jats:title>Background</jats:title> <jats:p>This study was designed to investigate the clinical application, efficacy, and safety of immune checkpoint inhibitors (ICIs) in the treatment of lung cancer in the real world.</jats:p> </jats:sec><jats:sec> <jats:title>Methods</jats:title> <jats:p>A retrospective, observational analysis was conducted on patients treated with ICIs in four tertiary hospitals in the region from January 2015 to March 2021, to evaluate the clinical efficacy of ICIs single-agent or combined chemotherapy and anti-vascular drugs in the first-line or second-line treatment of patients with lung cancer.</jats:p> </jats:sec><jats:sec> <jats:title>Results</jats:title> <jats:p>Three hundred and fifteen patients were enrolled in this study. In patients with stage III-IV adenocarcinoma and Squamous cell carcinoma, the objective response rate (ORR) and disease control rate (DCR) were 35.5% (87/245) and 93.5% (229/245), respectively, the median progression-free survival (PFS) was 10.8 months, and the median overall survival (OS) was not reached. A total of 132 patients received ICIs as the first-line treatment, the median treatment cycle was 8 cycles (2–20 cycles), the short-term efficacy ORR was 38.6%, DCR was 93.9%, and the median PFS was 11.4 months. One hundred thirteen patients received ICIs treatment as second-line treatment, the median treatment cycle was five cycles (2–10 cycles), the short-term efficacy ORR was 31.9%, DCR was 92.9%, and the median PFS was 10.0 months. There were no statistically significant differences in ORR, DCR, or median PFS with ICIs as the first-line treatment compared with the second-line treatment(<jats:italic>P</jats:italic> &gt; 0.05). The results of subgroup analysis showed that Eastern Cooperative Oncology Group performance status (ECOG PS), epidermal growth factor receptor (EGFR) mutation status, pathological type and number of treatment lines were not correlated with median PFS(<jats:italic>P</jats:italic> &gt; 0.05). However, there were statistically significant differences in programmed death-ligand 1(PD-L1) expression, corticosteroid interference, and antibiotic (Abx) treatment among all groups (<jats:italic>P</jats:italic> &lt; 0.05). In terms of safety, the overall incidence of adverse reactions in 315 patients was 62.5%, and the incidence of immune-related adverse events (irAEs) was 13.7%. Grade 1–2 and 3–4 incidence of adverse events were 34.9 and 27.65%, respectively. There were four patients who experienced fatal irAEs, two cases were liver damage leading to liver failure, one case was immune related pneumonia, and one case was immune related myocarditis.</jats:p> </jats:sec><jats:sec> <jats:title>Conclusion</jats:title> <jats:p>In the real world, ICIs has a good effect on patients with lung cancer and significantly improves ORR and PFS.</jats:p> </jats:sec> <<<
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14.
龙海晨 (2023-06-25 00:06):
#paper Haichen Long, Yangyang Li, Huijuan Wang, Bingxuan Guo, Shuyan Song, Xiangyi Zhe, Hongtao Li, Dongmei Li, Renfu Shao, Zemin Pan . C/EBPβ expression decreases in cervical cancer and leads to tumorigenesis. BMC Cancer. 2023 Jan 24;23(1):79. doi: 10.1186/s12885-023-10543-9. PMID: 36694148; PMCID: PMC9872280. 这是我第一次作为第一作者在中科院Top期刊上发的文章,也是2023年发的第二篇文章。是群里分享的第三篇我作为作者的文章。目的是研究C/EBPβ蛋白在宫颈肿瘤发生和发展中的作用。整个过程中,我们采用定量RT-PCR分析临床标本(10例宫颈癌组织标本和10例相应正常宫颈组织标本)中C/EBPβ、miR-661和MTA1 mRNA的表达。应用免疫组织化学方法分析381例临床标本C/EBPβ、80例临床标本Ki67和60例临床标本PCNA蛋白的表达。采用MALDI-TOF MassARRAY分析C/EBPβ基因甲基化(13例宫颈癌症组织和13例相应的正常宫颈组织)。采用CCK-8分析宫颈癌症细胞系的细胞增殖情况。采用蛋白质印迹和免疫组织化学方法检测C/EBPβ蛋白的表达水平,并用定量RT-PCR分析mRNA的表达。采用流式细胞术检测细胞周期分布和细胞凋亡。进行集落形成、Transwell、细胞侵袭和伤口愈合测定以检测细胞迁移和侵袭。 通过实验,我们证明了,C/EBPβ在宫颈癌症组织中降低,C/EBP-β基因在宫颈癌症细胞中的过度表达可以抑制增殖、侵袭和迁移。
Abstract:
BACKGROUND: Cervical cancer is currently estimated to be the fourth most common cancer among women worldwide and the leading cause of cancer-related deaths in some of the world's poorest countries. … >>>
BACKGROUND: Cervical cancer is currently estimated to be the fourth most common cancer among women worldwide and the leading cause of cancer-related deaths in some of the world's poorest countries. C/EBPβ has tumor suppressor effects because it is necessary for oncogene-induced senescence. However, C/EBPβ also has an oncogenic role. The specific role of C/EBPβ in cervical cancer as a tumor suppressor or oncoprotein is unclear. OBJECTIVE: To explore the role of the C/EBPβ protein in cervical tumorigenesis and progression. METHODS: Quantitative RT-PCR was used to analyze C/EBPβ (15 cervical cancer tissue samples and 15 corresponding normal cervical tissue samples), miR-661, and MTA1 mRNA expression in clinical samples (10 cervical cancer tissue samples and 10 corresponding normal cervical tissue samples). Immunohistochemistry was used to analyze C/EBPβ (381 clinical samples), Ki67 (80 clinical samples) and PCNA ( 60 clinical samples) protein expression. MALDI-TOF MassARRAY was used to analyze C/EBPβ gene methylation (13 cervical cancer tissues and 13 corresponding normal cervical tissues). Cell proliferation was analyzed by CCK-8 in cervical cancer cell lines. Western blotting and immunohistochemistry were performed to detect C/EBPβ protein expression levels, and mRNA expression was analyzed by quantitative RT-PCR analysis. Flow cytometry was performed to measure cell cycle distribution and cell apoptosis. Colony formation, Transwell, cell invasion, and wound healing assays were performed to detect cell migration and invasion. RESULTS: C/EBPβ protein expression was significantly reduced in cervical cancer tissues compared with cervicitis tissues (P < 0.01). Ki67 protein and PCNA protein expression levels were significantly higher in cervical cancer tissues compared with cervicitis tissues. The rate of C/EBPβ gene promoter methylation of CpG12, 13, 14 and CpG19 in cervical cancer tissues was significantly increased compared with normal cervical tissue (P < 0.05). In addition, C/EBPβ was overexpressed in cervical cancer cells and this overexpression inhibited cell proliferation, migration, invasion, arrested cells in S phase, and promoted apoptosis. CONCLUSIONS: We have demonstrated that C/EBPβ decreased in cervical cancer tissues and overexpression of the C/EBPβ gene in cervical cancer cells could inhibit proliferation, invasion and migration. <<<
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15.
龙海晨 (2023-06-24 23:55):
#paper Weinan Zheng, Fuyuan Jin, Fang Wang, Luyue Wang, Shaowei Fu, Zemin Pan, Haichen Long. Analysis of eEF1A2 gene expression and copy number in cervical carcinoma. Medicine (Baltimore). 2023 Jan 13;102(2):e32559. doi: 10.1097/MD.0000000000032559. PMID: 36637958; PMCID: PMC9839279. 这是我第一次作为独立通讯作者发的文章也是2023年发的第一篇文章。是群里分享的第二篇我作为作者的文章。因为是阴性结果,没有发到太好的杂志。杂志要求补实验数据时,赶上疫情,封在家里,所以用生物信息学补的。这是一篇分子生物学和生物信息学相结合的文章。主要研究eEF1A2基因拷贝数与宫颈癌患者临床分期、病理分级和患者生存率之间的关系。QPCR分析样本拷贝数。生物信息学数据库分析相关数据。发现,eEF1A2基因在癌症组织中发生突变。eEF1A2基因拷贝数与子宫颈癌症组织中eEF1A1基因表达的变化无关。
Medicine, 2023-Jan-13. DOI: 10.1097/MD.0000000000032559 PMID: 36637958
Abstract:
OBJECTIVE: To explore and analyze the expression of eukaryotic translation elongation factor 1 alpha 2 (eEF1A2) gene in cervical cancer tissues, its relationship with patient survival, gene mutations, and changes … >>>
OBJECTIVE: To explore and analyze the expression of eukaryotic translation elongation factor 1 alpha 2 (eEF1A2) gene in cervical cancer tissues, its relationship with patient survival, gene mutations, and changes in copy number in cervical cancer and chronic cervicitis tissues. METHODS: The expression of the eEF1A2 gene in cervical cancer and its relationship with patient survival were analyzed using gene expression profile interactive analysis. Changes in eEF1A2 expression in cervical cancer tissues were analyzed using cBioPortal, a portal for cancer genomics analysis. The eEF1A2 copy number in cervical cancer tissues and chronic cervicitis tissues was determined by real-time fluorescence quantitative polymerase chain reaction. The relationship between the expression of eEF1A2 protein and the clinical stage, pathological grade, and patient survival of cervical cancer was analyzed by the database: The Human Protein Atlas, an integrated repository portal for tumor-immune system interactions. RESULTS: Gene expression profile interactive analysis database analysis showed no significant differences in the expression of eEF1A2 between cervical cancer and normal cervical tissues (P > .05). The eEF1A2 gene expression level was not correlated with the survival of cervical cancer patients (P > .05). Analysis of the cBioPortal database showed that 18 of 297 cervical cancer patients had eEF1A2 gene changes, including missense mutation, splice mutation, amplification, and messenger RNA increase. There was no significant difference in eEF1A2 gene copy number between cervical cancer and chronic cervicitis (P > .05). The Human Protein Atlas and an integrated repository portal for tumor-immune system interactions database analysis of immunohistochemical data showed that eEF1A2 protein expression was no significant difference in clinical stage, pathological grade and patient survival of cervical cancer (P > .05). CONCLUSION: The eEF1A2 gene was mutated in cervical cancer tissues. The eEF1A2 gene copy number was not associated with changes in the expression of the eEF1A2 gene in cervical cancer tissues. <<<
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16.
龙海晨 (2023-05-23 02:52):
#paper Sun M, Ji H, Xu N, Jiang P, Qu T, Li Y. Real-world data analysis of immune checkpoint inhibitors in stage III-IV adenocarcinoma and squamous cell carcinoma. BMC Cancer. 2022 Jul 13;22(1):762. doi: 10.1186/s12885-022-09843-3. PMID: 35831785; PMCID: PMC9277844. 文章对肺癌的鳞癌和腺癌患者接受免疫检查抑制治疗immune checkpoint inhibitors (ICIs)进行回顾性研究,评估ICIs的疗效和安全性。发现,ICIs对癌症患者有良好的疗效,并显著改善ORR和PFS。objective response rate (ORR) ,客观缓解率,是一种直接衡量药物抗肿瘤活性的指标,ORR反应了肿瘤药物治疗后,肿瘤缩小或被消灭的概率。无进展生存期(progression free survival,PFS)这个名词通常用在中晚期癌症,肿瘤侵犯范围比较大,或是发生转移的病人。病人的治疗目的是控制癌细胞生长不要继续恶化,改善病人的生活品质及延长生命。例如,4期肺癌病人接受标靶药物治疗,一年无进展生存期几率是30%,表示开始治疗追踪一年后,有3成的病人能够控制住肺癌。
Abstract:
BACKGROUND: This study was designed to investigate the clinical application, efficacy, and safety of immune checkpoint inhibitors (ICIs) in the treatment of lung cancer in the real world. METHODS: A … >>>
BACKGROUND: This study was designed to investigate the clinical application, efficacy, and safety of immune checkpoint inhibitors (ICIs) in the treatment of lung cancer in the real world. METHODS: A retrospective, observational analysis was conducted on patients treated with ICIs in four tertiary hospitals in the region from January 2015 to March 2021, to evaluate the clinical efficacy of ICIs single-agent or combined chemotherapy and anti-vascular drugs in the first-line or second-line treatment of patients with lung cancer. RESULTS: Three hundred and fifteen patients were enrolled in this study. In patients with stage III-IV adenocarcinoma and Squamous cell carcinoma, the objective response rate (ORR) and disease control rate (DCR) were 35.5% (87/245) and 93.5% (229/245), respectively, the median progression-free survival (PFS) was 10.8 months, and the median overall survival (OS) was not reached. A total of 132 patients received ICIs as the first-line treatment, the median treatment cycle was 8 cycles (2-20 cycles), the short-term efficacy ORR was 38.6%, DCR was 93.9%, and the median PFS was 11.4 months. One hundred thirteen patients received ICIs treatment as second-line treatment, the median treatment cycle was five cycles (2-10 cycles), the short-term efficacy ORR was 31.9%, DCR was 92.9%, and the median PFS was 10.0 months. There were no statistically significant differences in ORR, DCR, or median PFS with ICIs as the first-line treatment compared with the second-line treatment(P > 0.05). The results of subgroup analysis showed that Eastern Cooperative Oncology Group performance status (ECOG PS), epidermal growth factor receptor (EGFR) mutation status, pathological type and number of treatment lines were not correlated with median PFS(P > 0.05). However, there were statistically significant differences in programmed death-ligand 1(PD-L1) expression, corticosteroid interference, and antibiotic (Abx) treatment among all groups (P < 0.05). In terms of safety, the overall incidence of adverse reactions in 315 patients was 62.5%, and the incidence of immune-related adverse events (irAEs) was 13.7%. Grade 1-2 and 3-4 incidence of adverse events were 34.9 and 27.65%, respectively. There were four patients who experienced fatal irAEs, two cases were liver damage leading to liver failure, one case was immune related pneumonia, and one case was immune related myocarditis. CONCLUSION: In the real world, ICIs has a good effect on patients with lung cancer and significantly improves ORR and PFS. <<<
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17.
龙海晨 (2023-04-10 13:25):
#paper Toama W, Wiederin J, Shanley R, Jewett P, Gu C, Shenoy C, Nijjar PS, Blaes AH. Impact of pectoralis muscle loss on cardiac outcome and survival in Cancer patients who received anthracycline based chemotherapy: retrospective study. BMC Cancer. 2022 Jul 13;22(1):763. doi: 10.1186/s12885-022-09882-w. PMID: 35831837; PMCID: PMC9281070. 文章回顾研究了几种癌症患者用蒽环类药物化疗后胸肌质量指数(pectoralis muscle mass index,PMI)与总体死亡率,主要心脏事件(Major Adverse Cardiovascular Events,MACE)生存率间的关系。(这里给大家解释几个专业名词MACE通俗讲解就是与心脏相关的不好的事情,例如,1,心脏原因引起的死亡;2,发生非致命的心肌梗死;3;发生非致命的心血管事件。具体点来说,日常遇到的,复发心绞痛、急性心肌梗死、严重心律失常、心力衰竭、冠心病死亡,心血管疾病事件,心衰,缺血性心血管事件,心源性死亡。PMI是计算胸肌的一个指标,有点类似于我们日常生活中的BMI,PMI:胸大肌面积 [cm 2 ]/身高2 [m 2 ])文章对474名癌症患者进行了回顾性分析,发现,接受蒽环类药物治疗的患者治疗前胸肌指数越高,发生 MACE 的风险越低。认为对化疗前检测PMI,尤其是对肌肉减少症患者化疗前进行干预预防能有效减少患者的MACE风险。
Abstract:
INTRODUCTION: The impact of pectoralis muscle mass index (PMI) on cardiac events is not well studied in cancer patients, especially in those who have received chemotherapy with high potential cardiac … >>>
INTRODUCTION: The impact of pectoralis muscle mass index (PMI) on cardiac events is not well studied in cancer patients, especially in those who have received chemotherapy with high potential cardiac toxicity such as anthracyclines. METHODS: Individuals aged ≥18 years with a diagnosis of breast cancer, sarcoma, or lymphoma who received anthracycline-based chemotherapy at the University of Minnesota MHealth Fairview between 2009 and 2014. Eligible patients had to have two CT scans: a baseline CT scan within 6 months prior to chemotherapy and a follow-up CT scan within 2 years after treatment. The PMI was calculated as the right pectoralis muscle area indexed to height squared. Multivariable linear regression was used to analyze factors associated with PMI at follow-up, overall mortality, and major cardiac events (MACE). RESULTS: A total of 474 patients (breast cancer 192; lymphoma 184; sarcoma 98) participated with a median age of 61 years at the time of baseline CT scan; 161 (34%) were male. Almost all patients received anthracyclines except 12% who received trastuzumab only. The median baseline PMI was 5.8 cm2/m2 (4.9, 7.7) which decreased 10.5% after chemotherapy, to 5.2 cm2/m2 (4.4, 6.4). Baseline PMI was not significantly associated with OS, but we detected lower risks of MACE with larger PMI at baseline. Greater baseline PMI was associated with greater follow-up PMI, but also with greater relative PMI loss. Female gender, older age, and history of smoking were also associated with greater PMI losses. CONCLUSION: Greater pre-treatment pectoralis muscle index in patients treated with anthracyclines have a lower risk of MACE. Early identification of sarcopenia using PMI could trigger proactive engagement for intervention and risk-stratified therapies. <<<
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18.
龙海晨 (2023-03-16 18:02):
#paper Yan Y, Ma Z, Ji X, Liu J, Ji K, Li S, Wu Q. A potential decision-making algorithm based on endoscopic ultrasound for staging early gastric cancer: a retrospective study. BMC Cancer. 2022 Jul 13;22(1):761. doi: 10.1186/s12885-022-09870-0. PMID: 35831843; PMCID: PMC9281103. 文章是研究内镜超声分析所得到的图像与胃癌早期分型间的关系,通过研究影像中肿瘤位置,是否胃溃疡,分化程度,肿瘤大小等指标,建立相关的算法,计算肿瘤分期,确定相应的治疗方案。为早期胃癌诊断治疗提供了一种决策算法。
Abstract:
BACKGROUND: Clinical staging of gastric cancer (GC) before treatment is essential. Endoscopic ultrasound (EUS) is a recommended staging tool, but its efficacy remains controversial. Our previous prospective study evaluated the … >>>
BACKGROUND: Clinical staging of gastric cancer (GC) before treatment is essential. Endoscopic ultrasound (EUS) is a recommended staging tool, but its efficacy remains controversial. Our previous prospective study evaluated the potential value of EUS for T staging and presented discrepancies. In this study, we aimed to evaluate the efficacy of EUS in T staging by comparing it with pathological staging. We analyze the factors that can potentially affect accuracy to identify suitable subgroups for EUS staging. METHODS: Data from a total of 1763 consecutive patients with GC from January 2015 to December 2017 were analyzed. Results from EUS and pathological T staging were compared. The factors that might affect EUS's accuracy were analyzed. RESULTS: The sensitivity, specificity, positive predictive value, and negative predictive value of EUS in patients with early GC were 62.08%, 96.13%, 90.94%, and 80.21%, respectively. The accuracy rates of uT1, uT2-uT4, and uT3-uT4 were 90.94%, 79.02%, and 78.39%, respectively. In multivariate analysis, underestimation was more likely to be observed in patients with tumors located in the middle or upper third of the stomach. Overestimation was more likely to be observed in patients with tumors located in the lower third or those without ulcer. Other factors affecting accuracy included ulcer, differentiation, larger size and undergoing surgery. CONCLUSION: Our findings highlight the role of EUS in determining the T staging of GC. Overestimation and underestimation in T-staging were significantly associated with the tumor location in early GC, and a decision-making algorithm was proposed for clinical practice in early cancers based on these findings. <<<
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19.
龙海晨 (2023-02-05 17:58):
#paper Feng H, Cao B, Peng X, Wei Q. Cancer-associated fibroblasts strengthen cell proliferation and EGFR TKIs resistance through aryl hydrocarbon receptor dependent signals in non-small cell lung cancer. BMC Cancer. 2022 Jul 13;22(1):764. doi: 10.1186/s12885-022-09877-7. PMID: 35831824; PMCID: PMC9281029.文章属于研究肿瘤的领域。进一步描述了CAF在癌症EGFR-TKIs耐药性发展中的作用。阐述了CAF与EGFR TKIs耐药相关的潜在机制,其依赖于Kyn/AhR/AKT/ERK信号通路。阻断AhR可有效改善EGFR TKIs的结果,为临床治疗癌症提供了一种新的策略。
Abstract:
The tumor microenvironment is a dynamic cellular milieu that interacts with cancer cells and promotes tumor progression and metastasis. However, the specific mechanisms by which the tumor microenvironment impacts cancer … >>>
The tumor microenvironment is a dynamic cellular milieu that interacts with cancer cells and promotes tumor progression and metastasis. However, the specific mechanisms by which the tumor microenvironment impacts cancer cells' behaviors remain poorly understood. In this study, enriched cancer-associated fibroblasts (CAFs) were observed in tumor tissues isolated from epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) resistant non-small cell lung cancer (NSCLC) patients. CAFs isolated from tumor tissues were capable of producing tryptophan metabolite kynurenine (Kyn), which significantly increased the proliferation and EGFR TKIs resistance of NSCLC cells. In this study, it was further observed that the activation of tryptophan 2,3-dioxygenase (TDO) in CAFs, resulted in the enhanced capability of tryptophan metabolism in them compared to normal fibroblasts. As a result, Kyn produced by CAFs facilitated the up-regulation of Aryl Hydrocarbon Receptor (AhR) signals in NSCLC, thereby resulting in the downstream ATK and ERK signaling pathways activation. Finally, inhibition of AhR signals efficiently prevented tumor growth and development of EGFR TKIs resistance, eventually improved the outcome of EGFR TKIs, and described a promising therapeutic strategy for NSCLC. <<<
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20.
龙海晨 (2023-01-02 13:45):
#paper Rosen D B, Murphy E A, Gejman R S, et al. Cytokine response over the course of COVID-19 infection in pregnant women[J]. Cytokine, 2022, 154: 155894. PMID: 35490452 PMCID: PMC9035355 DOI: 10.1016/j.cyto.2022.155894 这是研究新冠的文章。发表于2022年。样本是2020年3月到4月纽约市医院的。说明一下,那时候的新冠还不是现在低毒的奥密克戎,是最早的新冠病毒。文章研究的是妊娠期孕妇感染新冠后的血清情况。还有针对相应的细胞因子的治疗。回想2020年初新闻上自媒体上好多是在新冠上黑美国。其实公共防疫各国国情不同。美国的情况把人关房子里老百姓不答应。但对于高精尖层面的研究,2020年3月的时候咱们的核酸检测都没普及。美国治疗方法都到了对应不同人群和不同细胞因子的研究。文章对比分析了新冠阴性和阳性的孕妇细胞因子的水平。以及阳性孕妇不同感染时期各种细胞因子的水平。发现晚期妊娠妇女的细胞因子谱随感染的时间进程而变化,并与临床严重程度相关。
Abstract:
OBJECTIVE: To study how severity and progression of coronavirus disease (COVID-19) affect cytokine profiles in pregnant women. MATERIALS AND METHODS: 69 third-trimester, pregnant women were tested for COVID-19 infection and … >>>
OBJECTIVE: To study how severity and progression of coronavirus disease (COVID-19) affect cytokine profiles in pregnant women. MATERIALS AND METHODS: 69 third-trimester, pregnant women were tested for COVID-19 infection and SARS-CoV-2 specific IgM and IgG antibodies. Patients were stratified according to SARS-CoV-2 Reverse Transcriptase-PCR (RT-PCR) status and serology (IgM and IgG) status. Cytokines G-CSF, HGF, IL-18, IL-1Ra, IL-2Ra, IL-8, and IP-10 were measured via ELISA. Retrospective chart review for COVID-19 symptoms and patient vitals was conducted, and cytokine levels were compared between SARS-CoV-2 positive and negative cohorts, by seronegative and seropositive infection, by time course since onset of infection, and according to NIH defined clinical severity. RESULTS: IL-18, IL-1Ra, and IP-10 increased in the 44 RT-PCR positive pregnant women compared to the 25 RT-PCR negative pregnant controls. Elevated cytokine levels were found in early infections, defined by positive RT-PCR and seronegative status, and higher cytokine levels were also associated with more severe disease. By IgM seroconversion, IL-8 and IP-10 returned to levels seen in uninfected patients, while IL-18 levels remained significantly elevated. CONCLUSION: Cytokine profiles of third-trimester pregnant women vary with the time course of infection and are correlated with clinical severity. <<<
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