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1.
孤舟蓑笠翁
(2025-09-11 16:58):
#paper 【doi】10.1126/science.adm7066;【发表年份】2025年;【期刊】Science;【标题】Machine learning–based penetrance of genetic variants。【内容总结】作者想解决“同一个基因突变有人得病、有人不得病”的难题,用传统“有病/没病”二分法算外显率常因样本小、偏倚大而失真,于是把 134 万例纽约 Mount Sinai 医院常规体检的血检、血压等 47 项实验室指标和年龄、性别、BMI 喂给极端梯度提升树模型,为 10 种常染色体显性遗传病(如家族性高胆固醇血症、肥厚型心肌病、多囊肾等)各训练出“疾病分数”——一个 0 到 1 的小数,越接近 1 表示模型越确信这人“现在已处于疾病状态”,越接近 0 则越健康;再把模型套在 2.9 万例有外显子测序的 BioMe 独立队列,给 31 个基因里的 1648 个罕见突变(143 个已判致病、96 个良性、1181 个意义不明 VUS、228 个功能缺失 LoF)算出“ML 外显率”,即携带者的平均疾病分数转化为得病概率;结果致病突变 ML 外显率中位数 0.52 远高于良性 0.28,VUS 居中约 0.46,LoF 与致病突变相仿;ML 外显率越高,携带者越早在血检或影像上出现异常,如高外显率 PKD 突变者肾过滤率平均低 40 mL/min,高外显率 FH 突变者 LDL 高 119 mg/dL,且与体外实验测得的 BRCA1 修复能力下降、LDLR 摄取 LDL 能力下降一致;相比传统“有病/没病”外显率只能取 0、0.5、1 几个离散值,ML 外显率给出 0–1 之间的小数,把 20% 因无法确诊而被传统法扔掉的突变也纳入评估,还能把 66 个高外显率 VUS 和 48 个高外显率 LoF 挑出来,其携带者多年随访确实出现相应器官损伤;作者又在英国生物银行复制出趋势,说明方法可迁移;整个流程只用医院常规化验单,不额外花钱,为遗传咨询提供量化、个体化的风险数字,也能帮实验室优先验证真正致病的 VUS。
Science,
2025-8-28.
DOI: 10.1126/science.adm7066
Abstract:
Accurate variant penetrance estimation is crucial for precision medicine. We constructed machine learning (ML) models for 10 diseases using 1,347,298 participants with electronic health records, then applied them to an …
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Accurate variant penetrance estimation is crucial for precision medicine. We constructed machine learning (ML) models for 10 diseases using 1,347,298 participants with electronic health records, then applied them to an independent cohort with linked exome data. Resulting probabilities were used to evaluate ML penetrance of 1648 rare variants in 31 autosomal dominant disease-predisposition genes. ML penetrance was variable across variant classes, but highest for pathogenic and loss-of-function variants, and was associated with clinical outcomes and functional data. Compared with conventional case-versus-control approaches, ML penetrance provided refined quantitative estimates and aided the interpretation of variants of uncertain significance and loss-of-function variants by delineating clinical trajectories over time. By leveraging ML and deep phenotyping, we present a scalable approach to accurately quantify disease risk of variants.
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2.
孤舟蓑笠翁
(2025-09-10 11:55):
paper 【doi】10.1016/j.cell.2025.08.018;【发表年份】2025年;【期刊】Cell;【标题】AI mirrors experimental science to uncover a mechanism of gene transfer crucial to bacterial evolution。【内容总结】这篇论文研究了一个AI系统如何帮助科学家发现细菌基因转移的新机制。简单来说,科学家用AI来猜细菌中一种叫cf-PICIs的基因元件是怎么在不同细菌种类之间传播的,结果AI猜对了——它发现这些基因元件会“偷”不同细菌病毒的尾巴来感染新宿主。具体来说,研究者先实验发现cf-PICIs能在多种细菌中存在,但不知道原因。他们用AI系统“AI co-scientist”分析这个问题,AI提出了五个可能解释,其中排名第一的“cf-PICIs利用不同噬菌体尾巴”正好是科学家们通过多年实验刚发现但还没发表的正确答案。AI还提出其他有趣假设,比如基因元件可能通过细菌“接合”方式传播,这些新想法现在也成了实验室的新研究方向。主要方法包括:1)用AI系统生成和排序假设;2)将AI结果与实验发现对比;3)比较不同AI模型的性能。结果表明AI不仅能重复人类科学家的发现,还能提出创新想法,帮助加速科研。
3.
孤舟蓑笠翁
(2025-09-09 14:54):
paper 【doi】10.1038/s41586-025-09445-6;【发表年份】2025年;【期刊】Nature;【标题】Mechanical confinement governs phenotypic plasticity in melanoma。【内容总结】这篇论文研究了黑色素瘤细胞如何在外界机械压力下从增殖状态转变为侵袭状态。简单来说,科学家发现当肿瘤细胞被周围组织挤压时,会像神经元一样长出保护性微管结构,同时激活HMGB2蛋白来改变染色质结构,使细胞变得更擅长移动但不太会分裂,还更耐药。具体来说,团队通过斑马鱼模型和人类样本发现,处于肿瘤边缘的细胞会形成椭圆形的受压细胞核,这些"界面细胞"表现出神经元样基因特征。他们开发了体外微限制系统模拟这种压力,发现受压细胞会形成乙酰化微管笼保护细胞核,并通过HMGB2蛋白改变染色质可及性。关键方法包括:空间转录组学、单细胞RNA测序、体外微限制实验、ATAC-seq分析染色质开放性和TurboID蛋白质互作分析。结果显示机械限制通过HMGB2介导的表型转换使黑色素瘤获得侵袭性和耐药性,这为理解肿瘤转移提供了新视角。
Nature,
2025-8-27.
DOI: 10.1038/s41586-025-09445-6
Abstract:
Abstract Phenotype switching is a form of cellular plasticity in which cancer cells reversibly move between two opposite extremes: proliferative versus invasive states1,2. Although it has long been hypothesized that …
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Abstract Phenotype switching is a form of cellular plasticity in which cancer cells reversibly move between two opposite extremes: proliferative versus invasive states1,2. Although it has long been hypothesized that such switching is triggered by external cues, the identity of these cues remains unclear. Here we demonstrate that mechanical confinement mediates phenotype switching through chromatin remodelling. Using a zebrafish model of melanoma coupled with human samples, we profiled tumour cells at the interface between the tumour and surrounding microenvironment. Morphological analysis of interface cells showed elliptical nuclei, suggestive of mechanical confinement by the adjacent tissue. Spatial and single-cell transcriptomics demonstrated that interface cells adopted a gene program of neuronal invasion, including the acquisition of an acetylated tubulin cage that protects the nucleus during migration. We identified the DNA-bending protein HMGB2 as a confinement-induced mediator of the neuronal state. HMGB2 is upregulated in confined cells, and quantitative modelling revealed that confinement prolongs the contact time between HMGB2 and chromatin, leading to changes in chromatin configuration that favour the neuronal phenotype. Genetic disruption of HMGB2 showed that it regulates the trade-off between proliferative and invasive states, in which confined HMGB2high tumour cells are less proliferative but more drug-resistant. Our results implicate the mechanical microenvironment as a mechanism that drives phenotype switching in melanoma.
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4.
孤舟蓑笠翁
(2025-09-08 09:19):
paper 【doi】10.1038/s41588-025-02326-8;【发表年份】2025年;【期刊】Nature Genetics;【标题】Genetic variants affecting RNA stability influence complex traits and disease risk。【内容总结】这篇论文研究了基因变异如何通过影响RNA稳定性来调控基因表达并导致疾病风险。科学家们开发了一个叫RNAtracker的计算工具,利用Bru-seq/BruChase-seq代谢标记数据(在11种人类细胞系中追踪RNA随时间变化),区分了由转录调控(asRT)和RNA稳定性调控(asRS)引起的等位基因特异性表达。具体来说,他们发现了5,051个asRS变异涉及665个基因,这些变异显著富集于microRNA靶标区域和RNA结合蛋白位点,并通过MapUTR大规模并行报告实验和CRISPR基因编辑验证了其功能性。特别值得注意的是,asRS基因在免疫相关通路中高度富集,并与多种免疫系统疾病风险相关,例如系统性红斑狼疮和1型糖尿病。这项研究揭示了RNA稳定性这一被忽视的调控机制在连接遗传变异与疾病中的关键作用。主要方法包括:1)RNAtracker算法用β-二项混合模型分析时间序列RNA数据;2)ActD转录抑制实验验证稳定性调控;3)MapUTR和MPRAu高通量筛选功能性变异;4)CRISPR prime editing直接验证因果变异;5)GWAS和TWAS分析疾病关联性。
5.
孤舟蓑笠翁
(2025-09-05 14:18):
paper 【doi】10.1038/s41586-025-09460-7;【发表年份】2025年;【期刊】Nature;【标题】Rewiring of cortical glucose metabolism fuels human brain cancer growth。【内容总结】这篇研究想搞清楚脑癌(特别是胶质母细胞瘤GBM)是怎么改变大脑正常葡萄糖代谢来促进自己生长的。科学家们给脑癌患者和小鼠注射带标记的葡萄糖,然后用质谱和代谢流分析等方法追踪这些葡萄糖在肿瘤和正常脑组织中的去向。他们发现:正常脑细胞主要用葡萄糖来维持生理功能,而癌细胞却把葡萄糖转向制造增殖所需的核苷酸等物质,还从环境中抢走丝氨酸等营养。通过限制饮食中的丝氨酸,可以减缓某些脑癌的生长并增强放疗效果。简单说,脑癌会"偷改"葡萄糖的使用说明书,把本该用于维持大脑正常工作的能量转变成让自己疯长的原料,而这个漏洞可以通过调整饮食来部分堵住。主要方法包括:稳定同位素标记(给病人和小鼠注射带碳13标记的葡萄糖)、代谢流分析(计算不同代谢路径的流量)、质谱检测(测量代谢物中的同位素分布)、以及饮食干预实验(测试限制丝氨酸/甘氨酸饮食的效果)。
6.
孤舟蓑笠翁
(2025-09-05 14:17):
paper 【doi】10.1038/s41586-025-09479-w;【发表年份】2025年;【期刊】Nature;【标题】Supervised learning in DNA neural networks。【内容总结】这篇论文的目标是让DNA分子像大脑一样通过学习完成复杂任务,比如识别手写数字。研究者设计了一种由DNA分子构成的神经网络,通过化学反应的"训练"阶段让DNA记住特定模式(如数字0和1的图片),然后在"测试"阶段用记住的模式对新输入进行分类。主要方法包括:1)用DNA链置换反应模拟神经网络计算;2)设计可激活的"记忆分子"存储学习内容;3)采用"赢家通吃"机制做决策。实验结果显示,这个DNA网络能成功学习100位模式(相当于10×10像素图片),分类准确率最高达71%。简单说就是科学家用DNA分子造了个会学习的小电脑,它能记住看到的图案,之后遇到类似图案时能认出来,虽然不如人脑厉害,但这是首次实现分子级别的自主学习系统。
Nature,
2025-9-3.
DOI: 10.1038/s41586-025-09479-w
Abstract:
No abstract available.
7.
孤舟蓑笠翁
(2025-09-04 10:30):
paper 【doi】10.1038/s41586-025-09435-8;【发表年份】2025年;【期刊】Nature;【标题】Single-cell transcriptomic and genomic changes in the ageing human brain。【内容总结】这篇论文研究了人类大脑前额叶皮层从婴儿期到百岁老人期间的细胞水平变化,目标是理解健康大脑衰老过程中基因表达和基因组突变的规律。研究者使用了三种单细胞技术:单核RNA测序(snRNA-seq)分析基因表达,单细胞全基因组测序(scWGS)检测体细胞突变,以及MERFISH空间转录组技术进行验证。他们发现婴儿大脑中存在表达神经发育基因的特殊神经元和星形胶质细胞集群,而衰老过程中管家基因(如核糖体和线粒体相关基因)普遍下调,但神经元特异性基因保持稳定。通过突变特征分析,他们鉴定出两种与年龄相关的突变模式:A1突变与高表达基因和活跃染色质区域相关,A2突变则富集于低表达区域。特别有趣的是,短管家基因在衰老过程中因高突变率而表达下降,而长神经元基因则受到拓扑异构酶的保护维持稳定。这些发现揭示了大脑衰老中基因长度、功能和DNA损伤之间的复杂关系,为理解认知衰退提供了新视角。
Abstract:
Over time, cells in the brain and in the body accumulate damage, which contributes to the ageing process. In the human brain, the prefrontal cortex undergoes age-related changes that can …
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Over time, cells in the brain and in the body accumulate damage, which contributes to the ageing process. In the human brain, the prefrontal cortex undergoes age-related changes that can affect cognitive functioning later in life. Here, using single-nucleus RNA sequencing (snRNA-seq), single-cell whole-genome sequencing (scWGS) and spatial transcriptomics, we identify gene-expression and genomic changes in the human prefrontal cortex across lifespan, from infancy to centenarian. snRNA-seq identified infant-specific cell clusters enriched for the expression of neurodevelopmental genes, as well as an age-associated common downregulation of cell-essential homeostatic genes that function in ribosomes, transport and metabolism across cell types. Conversely, the expression of neuron-specific genes generally remains stable throughout life. These findings were validated with spatial transcriptomics. scWGS identified two age-associated mutational signatures that correlate with gene transcription and gene repression, respectively, and revealed gene length- and expression-level-dependent rates of somatic mutation in neurons that correlate with the transcriptomic landscape of the aged human brain. Our results provide insight into crucial aspects of human brain development and ageing, and shed light on transcriptomic and genomic dynamics.
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8.
孤舟蓑笠翁
(2025-09-03 21:40):
paper 【doi】10.1038/s41556-025-01751-5;【发表年份】2025年;【期刊】Nature Cell Biology;【标题】Genome-wide CRISPR screen identifies Menin and SUZ12 as regulators of human developmental timing。【内容总结】这篇论文研究了人类胚胎发育速度比其它物种慢的原因,通过全基因组CRISPR筛选发现表观遗传调控因子Menin和SUZ12是关键计时器。研究人员首先建立了PAX6::H2B-GFP报告系统监测神经分化速度,然后用全基因组CRISPR-Cas9敲除筛选找到27个候选基因,其中Menin和SUZ12缺失使神经分化显著加速。通过RNA测序、ATAC-seq和CUT&RUN等技术发现,这两个因子通过维持发育基因启动子区H3K4me3(激活标记)和H3K27me3(抑制标记)的平衡来控制分化速度:当Menin缺失时H3K4me3在bivalent启动子区增加,而SUZ12缺失则导致H3K27me3全局减少,都使发育基因更易被激活。这种加速效应不仅见于神经外胚层,在内胚层分化(GATA6/CXCR4表达提前)和心肌细胞分化(收缩提前)中也得到验证,说明这是跨胚层的通用计时机制。研究还发现小分子抑制剂VTP50469(靶向Menin-MLL)和Tazemetostat(靶向EZH2)能模拟基因敲除效果,为人工调控发育速度提供了工具。
Nature Cell Biology,
2025-9-2.
DOI: 10.1038/s41556-025-01751-5
Abstract:
Abstract Embryonic development follows a conserved sequence of events across species, yet the pace of development is highly variable and particularly slow in humans. Species-specific developmental timing is largely recapitulated …
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Abstract Embryonic development follows a conserved sequence of events across species, yet the pace of development is highly variable and particularly slow in humans. Species-specific developmental timing is largely recapitulated in stem cell models, suggesting a cell-intrinsic clock. Here we use directed differentiation of human embryonic stem cells into neuroectoderm to perform a whole-genome CRISPR-Cas9 knockout screen and show that the epigenetic factors Menin and SUZ12 modulate the speed of PAX6 expression during neural differentiation. Genetic and pharmacological loss-of-function of Menin or SUZ12 accelerate cell fate acquisition by shifting the balance of H3K4me3 and H3K27me3 at bivalent promoters, thereby priming key developmental genes for faster activation upon differentiation. We further reveal a synergistic interaction of Menin and SUZ12 in modulating differentiation speed. The acceleration effects were observed in definitive endoderm, cardiomyocyte and neuronal differentiation paradigms, pointing to chromatin bivalency as a general driver of timing across germ layers and developmental stages.
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9.
孤舟蓑笠翁
(2025-09-02 21:53):
paper 【doi】10.1038/s41586-025-09419-8;【发表年份】2025年;【期刊】Nature;【标题】Maternal stress triggers early-life eczema through fetal mast cell programming。【内容总结】这篇研究想搞清楚为什么妈妈怀孕时压力大会让宝宝出生后容易得湿疹。科学家用小鼠做实验,发现妈妈压力大会让体内皮质酮激素升高,这种激素会影响宝宝皮肤里一种叫肥大细胞的免疫细胞,让它们变得敏感,稍微摩擦皮肤就会发炎长湿疹。主要方法包括:给怀孕小鼠施加压力(每天关管子+强光照射)、测量激素水平、用显微镜观察皮肤细胞、做基因测序分析细胞变化。结果发现:1) 压力妈妈的小鼠宝宝皮肤肥大细胞在子宫里就被激活了;2) 这些宝宝出生后皮肤神经对触摸更敏感;3) 阻断妈妈的压力激素能预防宝宝湿疹;4) 人类数据也显示过敏体质的孕妇压力激素更高。简单说就是妈妈压力→激素变化→宝宝皮肤细胞程序出错→轻轻摩擦就长湿疹,但长大后会自己好转。
10.
孤舟蓑笠翁
(2025-08-29 16:17):
paper 【doi】10.1038/s41586-025-09399-9;【发表年份】2025年;【期刊】Nature;【标题】The evolution of hominin bipedalism in two steps。【内容总结】这篇论文研究了人类两足行走的进化基础,特别是骨盆形状的变化机制。简单说,科学家想搞清楚人类骨盆为什么比其他灵长类更短更宽,从而支持直立行走。他们用了多种方法:比较了人类、小鼠和多种灵长类(如黑猩猩、鼠狐猴)的骨盆发育过程;通过显微CT扫描观察骨骼生长;用单细胞多组学分析基因表达;还研究了SOX9、PTH1R等基因的作用。结果发现人类骨盆发育有两个关键变化:一是软骨生长板方向从纵向变为横向,使骨盆变宽;二是骨化(骨头形成)从后部开始并延迟,保留了复杂形状。详细来说,团队通过比较解剖学发现人类髂骨生长板方向与其他灵长类不同,基因分析显示SOX9-PTH1R信号通路和RUNX2等基因调控了这些变化,空间转录组数据证实肌肉早期附着影响了骨盆形态。这些改变共同使人类骨盆能支撑直立行走,同时容纳大脑较大的婴儿。
11.
孤舟蓑笠翁
(2025-08-29 16:07):
paper 【doi】10.1016/j.cell.2025.07.042;【发表年份】2025年;【期刊】Cell;【标题】Tumor transcriptome-wide expression classifiers predict treatment sensitivity in advanced prostate cancers。【内容总结】这篇研究想找出能预测晚期前列腺癌患者对不同治疗(如激素疗法和化疗)反应的生物标志物,通过分析1523名患者的肿瘤RNA表达数据(使用微阵列技术检测基因表达水平),结合长期生存随访数据,发现高Decipher评分(反映肿瘤增殖活性)和PTEN失活(通过PTEN_loss_Liu转录组特征判断)的肿瘤对化疗药物多西他赛更敏感,而雄激素受体(AR)信号强的肿瘤生存期更长。主要方法包括:临床级微阵列检测肿瘤转录组、免疫组化验证(Ki-67和PTEN蛋白)、多变量Cox比例风险模型分析生存关联、预定义的生物标志物-治疗交互作用检验。结果显示Decipher评分高的转移性患者接受多西他赛后死亡风险降低36%(HR=0.64),PTEN失活肿瘤的死亡风险降低43%(HR=0.57),而AR信号活跃与更好预后相关(非转移患者HR=0.58)。
12.
孤舟蓑笠翁
(2025-08-29 15:51):
paper 【doi】10.1038/s41586-025-09462-5;【发表年份】2025年;【期刊】Nature;【标题】Haematopoietic stem cell number is not solely defined by niche availability。【内容总结】这项研究挑战了传统观点,发现造血干细胞(HSC)数量不仅由骨髓微环境(niche)容量决定,还受系统性调控。研究者开发了创新的股骨移植技术(将野生型小鼠股骨移植到未处理受体小鼠体内来增加niche数量),同时结合局部照射(选择性破坏特定骨骼区域的niche)、联体共生实验(连接两只小鼠的循环系统)和TPO基因修饰小鼠模型(改变血小板生成素水平)。主要发现包括:1)即使增加niche数量,HSC总数仍保持不变;2)当内源性niche功能缺陷时,移植niche中的HSC数量也不会超过生理水平;3)血小板生成素(TPO)是决定全身HSC总量的关键调控因子。这些结果更新了Schofield于1978年提出的niche理论,揭示了HSC数量受系统性(全身TPO水平)和局部(niche内)双重限制的新机制。
13.
孤舟蓑笠翁
(2025-08-27 07:47):
paper 【doi】10.1038/s41588-025-02289-w;【发表年份】2025年;【期刊】Nature Genetics;【标题】ERG-driven prostate cancer initiation is cell-context dependent and requires KMT2A and DOT1L。【内容总结】这篇研究想搞清楚为什么前列腺癌中常见的ERG基因突变会导致癌症发生,发现关键在于ERG只在特定类型的基底细胞(Basal lum细胞)中才会引发癌症。研究者用小鼠模型做了细胞谱系追踪、单细胞RNA测序和染色质可及性分析,发现这些特殊基底细胞被ERG激活后会先变成一种高增殖的中间态细胞(IM细胞),再发展成癌症;还发现IM细胞依赖STAT3、KMT2A和DOT1L这些因子,如果用CRISPR敲除这些基因就能阻止癌症发生。简单说就是:ERG致癌需要特定细胞环境,在Basal lum细胞里会通过IM细胞阶段发展成癌,这个过程需要KMT2A和DOT1L参与;而单细胞技术帮助发现了传统方法可能忽略的关键细胞亚群和机制。具体来说,他们用转基因小鼠模型结合荧光标记追踪了不同前列腺上皮细胞的命运,通过流式细胞术和免疫荧光发现只有同时表达基底和管腔标记的Basal lum细胞才是ERG致癌的起源;单细胞测序揭示IM细胞具有独特的基因表达谱和开放的染色质区域(特别富含STAT3和ETS家族转录因子结合位点);最后用CRISPR在类器官和移植模型中验证了STAT3、KMT2A/MLL1和DOT1L对ERG致癌的关键作用,这为靶向治疗提供了新思路。
Nature Genetics,
2025-8-26.
DOI: 10.1038/s41588-025-02289-w
Abstract:
Abstract Despite the high prevalence of ERG transcription factor translocations in prostate cancer, the mechanism of tumorigenicity remains poorly understood. Using lineage tracing, we find the tumor-initiating activity of ERG …
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Abstract Despite the high prevalence of ERG transcription factor translocations in prostate cancer, the mechanism of tumorigenicity remains poorly understood. Using lineage tracing, we find the tumor-initiating activity of ERG resides in a subpopulation of murine basal cells that coexpress luminal genes (BasalLum) and not in the larger population of ERG+ luminal cells. Upon ERG activation, BasalLum cells give rise to highly proliferative intermediate (IM) cells with stem-like features that coexpress basal, luminal, hillock and club marker genes, before transitioning to Krt8+ luminal cells. Transcriptomic analysis of ERG+ human prostate cancers confirms the presence of rare ERG+ BasalLum cells, as well as IM cells whose presence is associated with a worse prognosis. Single-cell analysis revealed a chromatin state in ERG+ IM cells enriched for STAT3 transcription factor binding sites and elevated expression of the KMT2A/MLL1 and DOT1L, all three of which are essential for ERG-driven tumorigenicity in vivo. In addition to providing translational opportunities, this work illustrates how single-cell approaches combined with lineage tracing can identify cancer vulnerabilities not evident from bulk analysis.
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14.
孤舟蓑笠翁
(2025-07-01 22:26):
#paper 【doi】10.1016/j.phymed.2025.156828;【发表年份】2025年;【期刊】Phytomedicine;【标题】Deciphering the molecular mechanisms of QLQX capsules in heart failure: A multi-omics perspective。【内容总结】这篇论文研究了中药QLQX胶囊对保留射血分数心衰(HFpEF)的治疗机制,简单说就是想搞清楚这个药为啥能改善心脏功能。研究者先用网络药理学预测了QLQX的活性成分和潜在靶点,然后通过大鼠实验(包括超声心动图、RNA测序、蛋白质组学和代谢组学)验证效果,发现QLQX能通过调节cGMP-PKG信号通路等改善心脏舒张功能。具体来说,他们先通过计算机分析找到QLQX的44种活性成分可能作用于530个靶点,其中38个与HFpEF相关;接着用手术+高盐饮食制造HFpEF大鼠模型,给不同剂量QLQX治疗8周后,用多组学方法发现药物显著提升了血清一氧化氮(NO)和环磷酸鸟苷(cGMP)水平,改善了心肌肥厚指标,并通过转录组发现216个基因表达被逆转,蛋白质组显示401个差异蛋白被调控,代谢组显示QLQX能纠正脂代谢异常。最终证明QLQX像"多面手"一样通过cGMP-PKG通路协调改善血管功能、钙离子平衡和能量代谢,这解释了它对复杂病因的HFpEF的疗效。
15.
孤舟蓑笠翁
(2025-06-30 19:17):
#paper 【doi】10.1016/j.ccell.2025.04.005;【发表年份】2025年;【期刊】Cancer Cell;【标题】Immune evolution in pre-invasive lung adenocarcinoma。【内容总结】这篇论文研究了肺腺癌前驱病变进展过程中免疫微环境的动态变化,目标是找到早期干预的治疗靶点。研究者使用空间单细胞分析(成像质谱技术)和五种小鼠模型,发现TIM-3免疫检查点在癌前阶段高表达于先天免疫细胞(如巨噬细胞、树突细胞),阻断TIM-3能显著减小小鼠模型的病变尺寸,但在侵袭期无效。具体来说,他们分析了114个人类肺腺癌前驱病变样本,通过成像质谱绘制了从非典型腺瘤性增生(AAH)到侵袭性腺癌(IAC)的免疫细胞组成与空间分布变化,发现先天免疫反应在癌前阶段占主导,而适应性免疫在侵袭期增强;小鼠实验显示TIM-3阻断在癌前阶段能减少M2型巨噬细胞、增加抗原呈递树突细胞,从而抑制肿瘤进展,但该效果具有阶段特异性。
Cancer Cell,
2025-6.
DOI: 10.1016/j.ccell.2025.04.005
Abstract:
No abstract available.
16.
孤舟蓑笠翁
(2025-06-29 09:36):
#paper 【doi】10.1038/s41586-025-09182-w;【发表年份】2025年;【期刊】Nature;【标题】In vivo mapping of mutagenesis sensitivity of human enhancers。【内容总结】这篇论文想搞清楚人类增强子(控制基因开关的DNA片段)中哪些小片段对胚胎发育最关键。科学家选了7个控制大脑、心脏和四肢发育的增强子,用转基因小鼠做实验:先把这些增强子切成12碱基的小块,用CRISPR技术突变每个小块,然后观察突变后胚胎器官发育的变化(主要看颜色标记的LacZ基因表达)。他们发现69%的小块突变会影响发育(60%让增强子失效,9%反而增强活性),并用机器学习模型预测出88%的关键位点与实验结果吻合。简单说就是:像拆乐高一样把增强子拆成小零件,发现大部分零件都重要,突变会搞乱发育;还训练AI模型来预测哪些零件最关键,结果挺准。主要方法包括:转基因小鼠胚胎实验(enSERT技术)、12bp块突变策略、ChromBPNet机器学习模型分析染色质开放信号、DeepLIFT算法定位关键碱基。
17.
孤舟蓑笠翁
(2025-06-29 09:18):
#paper 【doi】10.1038/s41586-025-09154-0;【发表年份】2025年;【期刊】Nature;【标题】Major expansion in the human niche preceded out of Africa dispersal。【内容总结】这篇论文研究了为什么现代人类(智人)大约5万年前能成功走出非洲并扩散到全球,而更早的非洲外扩散(如12.5万年前)却未能留下遗传痕迹。研究者通过分析非洲479个放射性定年的考古遗址(图1显示分布),结合两种古气候模型(HadCM3和PCESM),使用物种分布模型(SDMs)和广义加性模型(GAMs)量化了人类生态位变化。结果发现,从约7万年前开始,人类逐渐适应了更广泛的生境(如森林和沙漠,图4b),这种生态灵活性在5万年前达到高峰,正好与成功扩散的时间吻合。研究认为,这种生态位扩张(可能是由于技术革新或人口压力)让人类能够应对走出非洲后遇到的各种环境挑战,而早期群体因适应性不足而失败。方法上,团队通过重采样解决考古数据的时间偏差(Extended Data Fig.1),并用主成分分析量化生态位空间变化(Extended Data Fig.3)。
Nature,
2025-6-18.
DOI: 10.1038/s41586-025-09154-0
Abstract:
Abstract All contemporary Eurasians trace most of their ancestry to a small population that dispersed out of Africa about 50,000 years ago (ka)1–9. By contrast, fossil evidence attests to earlier …
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Abstract All contemporary Eurasians trace most of their ancestry to a small population that dispersed out of Africa about 50,000 years ago (ka)1–9. By contrast, fossil evidence attests to earlier migrations out of Africa10–15. These lines of evidence can only be reconciled if early dispersals made little to no genetic contribution to the later, major wave. A key question therefore concerns what factors facilitated the successful later dispersal that led to long-term settlement beyond Africa. Here we show that a notable expansion in human niche breadth within Africa precedes this later dispersal. We assembled a pan-African database of chronometrically dated archaeological sites and used species distribution models (SDMs) to quantify changes in the bioclimatic niche over the past 120,000 years. We found that the human niche began to expand substantially from 70 ka and that this expansion was driven by humans increasing their use of diverse habitat types, from forests to arid deserts. Thus, humans dispersing out of Africa after 50 ka were equipped with a distinctive ecological flexibility among hominins as they encountered climatically challenging habitats, providing a key mechanism for their adaptive success.
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18.
孤舟蓑笠翁
(2025-06-29 09:11):
#paper 【doi】10.1038/s41586-025-09151-3;【发表年份】2025年;【期刊】Nature;【标题】Morphodynamics of human early brain organoid development。【内容总结】这项研究想了解人类大脑早期发育过程中细胞外基质(ECM)如何影响脑类器官的形状变化和区域形成。科学家们开发了新技术:1)用荧光标记不同细胞结构(细胞膜、细胞骨架等),2)用光片显微镜长期观察类器官生长,3)计算机分析细胞形状变化。他们发现:1)脑类器官发育分三个阶段(快速生长、腔室融合、成熟),2)添加外源ECM(如Matrigel)能帮助形成更规则的神经上皮结构,3)缺少ECM会导致类器官发育异常,出现更多神经嵴细胞,4)ECM通过WNT和Hippo信号通路影响类器官发育。具体来说,研究团队标记了肌动蛋白、微管等结构,用显微镜追踪数周,发现ECM能促进细胞排列整齐和腔室融合;没有ECM时,YAP1蛋白会上调,激活WNT通路基因WLS,导致类器官尾部化(更像脊髓而不是大脑)。这些发现帮助我们理解ECM在人类大脑发育中的重要作用。
Nature,
2025-6-18.
DOI: 10.1038/s41586-025-09151-3
Abstract:
Abstract Brain organoids enable the mechanistic study of human brain development and provide opportunities to explore self-organization in unconstrained developmental systems1–3. Here we establish long-term, live light-sheet microscopy on unguided …
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Abstract Brain organoids enable the mechanistic study of human brain development and provide opportunities to explore self-organization in unconstrained developmental systems1–3. Here we establish long-term, live light-sheet microscopy on unguided brain organoids generated from fluorescently labelled human induced pluripotent stem cells, which enables tracking of tissue morphology, cell behaviours and subcellular features over weeks of organoid development4. We provide a novel dual-channel, multi-mosaic and multi-protein labelling strategy combined with a computational demultiplexing approach to enable simultaneous quantification of distinct subcellular features during organoid development. We track actin, tubulin, plasma membrane, nucleus and nuclear envelope dynamics, and quantify cell morphometric and alignment changes during tissue-state transitions including neuroepithelial induction, maturation, lumenization and brain regionalization. On the basis of imaging and single-cell transcriptome modalities, we find that lumenal expansion and cell morphotype composition within the developing neuroepithelium are associated with modulation of gene expression programs involving extracellular matrix pathway regulators and mechanosensing. We show that an extrinsically provided matrix enhances lumen expansion as well as telencephalon formation, and unguided organoids grown in the absence of an extrinsic matrix have altered morphologies with increased neural crest and caudalized tissue identity. Matrix-induced regional guidance and lumen morphogenesis are linked to the WNT and Hippo (YAP1) signalling pathways, including spatially restricted induction of the WNT ligand secretion mediator (WLS) that marks the earliest emergence of non-telencephalic brain regions. Together, our work provides an inroad into studying human brain morphodynamics and supports a view that matrix-linked mechanosensing dynamics have a central role during brain regionalization.
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19.
孤舟蓑笠翁
(2025-06-29 09:01):
#paper 【doi】;【发表年份】2025年;【期刊】Nature;【标题】Targeting de novo purine biosynthesis for tuberculosis treatment。【内容总结】这篇研究想解决结核病治疗中药物耐药和疗程长的问题,科学家们发现了一种叫JNJ-6640的新药,它能精准打击结核菌制造嘌呤(DNA的原料)的第一步关键酶PurF。他们先筛了4924种化合物找到苗头,再改进结构让药效更强(MIC90=8.6 nM),用基因敲除、显微镜看细菌分裂、测肺里化学物质浓度等方法证明这药只杀结核菌不伤人类细胞。重要发现包括:药能让结核菌的DNA复制停工(看图3的细菌分裂动画),肺里的嘌呤原料太少救不了被药打击的细菌(表1数据),动物实验用长效针剂打两针就能减少近99%病菌。这药还能替换现有方案里毒性大的药物,可能让未来治疗更短更安全。简单说就是:找到新靶点→设计特效药→证明它管用且安全→能改进现有疗法。
20.
孤舟蓑笠翁
(2025-06-29 08:54):
#paper 【doi】10.1038/s41586-025-09160-2;【发表年份】2025年;【期刊】Nature;【标题】Machine-learning design of ductile FeNiCoAlTa alloys with high strength。【内容总结】这篇论文的目标是开发同时具备高强度和高延展性的新型合金,突破传统合金强度与延展性此消彼长的限制。研究者采用机器学习结合领域知识(如原子尺寸错配最大化、L12/B2相调控)设计了Fe35Ni29Co21Al12Ta3高熵合金,通过冷轧和热处理工艺获得含66.6% L12纳米沉淀相和15% B2微米相的双相结构。实验结果惊人:屈服强度达1.8GPa的同时保持25%均匀延伸率,远超现有钢材和传统高熵合金。具体而言,团队首先构建包含20种物理特征的数据库,通过六步主动学习循环(数据收集→物性描述符筛选→模型训练→知识引导虚拟筛选→效用函数设计→实验验证)优化成分,最终材料中L12相提供主要强化(859MPa),可变形B2相通过位错积累维持高加工硬化率,两者协同作用使性能突破现有材料极限。关键创新在于机器学习指导下的多组元沉淀相设计,以及通过高体积分数可变形B2相(传统硬脆相经Ta合金化后变为韧性相)实现强度-延展性协同提升。