当前共找到 1276 篇文献分享,本页显示第 621 - 640 篇。
621.
笑对人生 (2023-04-30 23:23):
#paper doi: 10.1038/s41591-023-02221-x. Comitani F, et al. Diagnostic classification of childhood cancer using multiscale transcriptomics. Nat Med. 2023 Mar;29(3):656-666. 研究背景:世界每年新增的儿童肿瘤患者大约40万。与成年人癌症不同的是,儿童肿瘤大多起源于胚胎组织,并且影响肿瘤发展的细胞类型是不同的。白血病是一种多发于儿童的肿瘤,比例约占1/3。再如,神经母细胞瘤,是一种高度异质性癌症,可始于婴儿和在儿童或青少年期间出现恶性进展,但少见于成年人。目前,尚未发现能用于所有儿童肿瘤诊断的全面分子生物标志物。转录组测序不仅能反映肿瘤的表达谱特征,而且可以能发现独立于基因组的肿瘤间差异。大多数已建立的转录组测序分类模型都是需要预标的有监督工具,因此难以发现一些复杂的表型变化。此外,瘤内异质性和肿瘤基质或免疫细胞浸润存在可能会导致在同一种肿瘤同时存在预后不良和预后良好的生物标志物。综上,有必要寻找以转录组测序为基础、灵活性高和适用于所有儿童肿瘤的生物标志物。 样本类型:聚类用数据集:2,178份儿童肿瘤样本、9,400成人肿瘤和1,735非癌组织。神经母细胞瘤转录可塑性验证样本8份。 数据类型:RNAseq和scRNAseq 研究主要内容:基于RNAseq建立一种名为RACCOON的自适应聚类方法,该方法能实现对肿瘤亚型进行无监督分类。通过比较不同类群的特征,发现儿童和成年肿瘤因年龄不同明显的差异,并且发现儿童转录紊乱性更高。接着研究者开发了一个名为OTTER的集成CNN分类器,并以RACCOON的聚类结果作为输入。与任何单一模型和以往发表的分类器相比,OTTER在所有指标上都表现更为优秀,并能高精确地对儿童肿瘤样本进行癌种类型、癌与非癌和亚型进行分类。更令人惊讶的是,该分类管道在低肿瘤纯度、高技术噪音和低测序深度(几百万个reads)下,仍能保持较高的准确度。总而言之,该研究提供了一个适用儿童肿瘤的通用分类器,并有望应用于其他的癌症类型。
IF:58.700Q1 Nature medicine, 2023-03. DOI: 10.1038/s41591-023-02221-x PMID: 36932241
Abstract:
The causes of pediatric cancers' distinctiveness compared to adult-onset tumors of the same type are not completely clear and not fully explained by their genomes. In this study, we used … >>>
The causes of pediatric cancers' distinctiveness compared to adult-onset tumors of the same type are not completely clear and not fully explained by their genomes. In this study, we used an optimized multilevel RNA clustering approach to derive molecular definitions for most childhood cancers. Applying this method to 13,313 transcriptomes, we constructed a pediatric cancer atlas to explore age-associated changes. Tumor entities were sometimes unexpectedly grouped due to common lineages, drivers or stemness profiles. Some established entities were divided into subgroups that predicted outcome better than current diagnostic approaches. These definitions account for inter-tumoral and intra-tumoral heterogeneity and have the potential of enabling reproducible, quantifiable diagnostics. As a whole, childhood tumors had more transcriptional diversity than adult tumors, maintaining greater expression flexibility. To apply these insights, we designed an ensemble convolutional neural network classifier. We show that this tool was able to match or clarify the diagnosis for 85% of childhood tumors in a prospective cohort. If further validated, this framework could be extended to derive molecular definitions for all cancer types. <<<
翻译
622.
小W (2023-04-30 23:21):
#paper doi: https://doi.org/10.1038/s43018-023-00533-y inferring early genetic progression in cancers with unobtainable premalignant disease. 作者基于原发肿瘤中亚克隆之间进化关系开发了 PhylogicNDT 方法,使用原发性肿瘤样本的外显子组测序数据来推断癌症的早期遗传进展。作者进行了以下分析,(1)PhylogicNDT 方法能够重现 HPV- HNSCC 的已知遗传进展(Califano 等人的HNSCC经验进展模型) ;(2)PhylogicNDT 方法能够揭示难以获得癌前组织的 HPV+ HNSCC 的癌前遗传进展;(3)比较了 PhylogicNDT 方法 HPV+ 和 HPV– 遗传进展差异;(4)不同驱动事件的进展时间。最后,作者讨论了他们的方法在癌症早期检测、干预和预后评估方面的潜在应用,并指出了一些局限性和未来的改进方向,如考虑肿瘤内空间异质性、增加更多类型的突变、改进突变特征等。 关于HPV+ HNSCC 的 一个观点:根据PhylogicNDT 方法结果提出 ,驱动事件可能发生在诊断之前 25-35 年或更早, HPV 整合位点出现在肿瘤发展的早期,并在整个肿瘤发展过程中发生额外的整合,HPV 整合是 HPV+ 癌症发生的主要贡献者。
IF:23.500Q1 Nature cancer, 2023-04. DOI: 10.1038/s43018-023-00533-y PMID: 37081260
Abstract:
Analysis of premalignant tissue has identified the typical order of somatic events leading to invasive tumors in several cancer types. For other cancers, premalignant tissue is unobtainable, leaving genetic progression … >>>
Analysis of premalignant tissue has identified the typical order of somatic events leading to invasive tumors in several cancer types. For other cancers, premalignant tissue is unobtainable, leaving genetic progression unknown. Here, we demonstrate how to infer progression from exome sequencing of primary tumors. Our computational method, PhylogicNDT, recapitulated the previous experimentally determined genetic progression of human papillomavirus-negative (HPV) head and neck squamous cell carcinoma (HNSCC). We then evaluated HPV HNSCC, which lacks premalignant tissue, and uncovered its previously unknown progression, identifying early drivers. We converted relative timing estimates of driver mutations and HPV integration to years before diagnosis based on a clock-like mutational signature. We associated the timing of transitions to aneuploidy with increased intratumor genetic heterogeneity and shorter overall survival. Our approach can establish previously unknown early genetic progression of cancers with unobtainable premalignant tissue, supporting development of experimental models and methods for early detection, interception and prognostication. <<<
翻译
623.
muton (2023-04-30 23:19):
#paper Amygdala and cortical gamma-band responses to emotional faces depend on the attended to valence https://arxiv.org/pdf/2304.05700.pdf 杏仁核被认为贡献于情绪面孔视觉加工中自下而上的注意偏好,然而其对于情绪的反应如何与自上而下的注意相互作用却并不清楚。并且,杏仁核对情绪和注意的反应与头皮脑电相比有多大程度相似也仍有待探究。因此作者分别记录了杏仁核脑区的颅内电极以及头皮脑电伽马段的脑电活动来探究面孔加工过程中情绪和注意的交互。结果发现,在情绪检测实验中杏仁核的高频伽马出现在以中性面孔作为识别目标时,当以负性面孔作为识别目标时,低频伽马在负性面孔出现时会显著增加,并且不仅局限于杏仁核,同时在后部脑区头皮脑电记录中也存在,且时间窗早于杏仁核。这一结果符合情绪加工的多通路模型,并且是从注意(自上而下)的角度发现了伽马波在加工情绪面孔中的作用。
Abstract:
The amygdala is assumed to contribute to a bottom-up attentional bias during visual processing of emotional faces. Still, how its response to emotion interacts with top-down attention is not fully … >>>
The amygdala is assumed to contribute to a bottom-up attentional bias during visual processing of emotional faces. Still, how its response to emotion interacts with top-down attention is not fully understood. It is also unclear if amygdala activity and scalp EEG respond to emotion and attention in a similar way. Therefore, we studied the interaction of emotion and attention during face processing in oscillatory gamma-band activity (GBA) in the amygdala and on the scalp. Amygdala signals were recorded via intracranial EEG (iEEG) in 9 patients with epilepsy. Scalp recordings were collected from 19 healthy participants. Three randomized blocks of angry, neutral, and happy faces were presented, and either negative, neutral, or positive expressions were denoted as targets. Both groups detected happy faces fastest and most accurately. In the amygdala, the earliest effect was observed around 170 ms in high GBA (105-117.5 Hz) when neutral faces served as targets. Here, GBA was higher for emotional than neutral faces. During attention to negative faces, low GBA (< 90 Hz) increased specifically for angry faces both in the amygdala and over posterior scalp regions, albeit earlier on the scalp (60 ms) than in the amygdala (210 ms). From 570 ms, amygdala high GBA (117.5-145 Hz) was also increased for both angry and neutral, compared to happy, faces. When positive faces were the targets, GBA did not differentiate between expressions. The present data reveal that attention-independent emotion detection in amygdala high GBA may only occur during a neutral focus of attention. Top-down threat vigilance coordinates widespread low GBA, biasing stimulus processing in favor of negative faces. These results are in line with a multi-pathway model of emotion processing and help specify the role of GBA in this process by revealing how attentional focus can tune timing and amplitude of emotional GBA responses. <<<
翻译
624.
小擎子 (2023-04-30 23:14):
#paper doi: 10.1038/s41592-021-01141-3 Nat Methods, 2021, Challenges in Benchmarking Metagenomic Profilers. 文献提出了一个研究宏基因组中会遇到的问题,即计算相对丰度时,不同生信工具给出的统计结果不同。区别就是有的结果是给出的序列丰度(DNA to DNA),有的结果给出的是物种丰度(DNA to Marker)。序列丰度和物种丰度的差别在于,有没有将物种的基因组大小考虑在其中。序列丰度是不考虑物种基因组大小的(如Kraken)。文章认为,基于物种丰度(即考虑物种基因组大小)的结果更具有解释性,建议严谨解释宏基因组分析结果,特别是从序列丰度得出的结果。
IF:36.100Q1 Nature methods, 2021-06. DOI: 10.1038/s41592-021-01141-3 PMID: 33986544 PMCID:PMC8184642
Abstract:
Accurate microbial identification and abundance estimation are crucial for metagenomics analysis. Various methods for classification of metagenomic data and estimation of taxonomic profiles, broadly referred to as metagenomic profilers, have … >>>
Accurate microbial identification and abundance estimation are crucial for metagenomics analysis. Various methods for classification of metagenomic data and estimation of taxonomic profiles, broadly referred to as metagenomic profilers, have been developed. Nevertheless, benchmarking of metagenomic profilers remains challenging because some tools are designed to report relative sequence abundance while others report relative taxonomic abundance. Here we show how misleading conclusions can be drawn by neglecting this distinction between relative abundance types when benchmarking metagenomic profilers. Moreover, we show compelling evidence that interchanging sequence abundance and taxonomic abundance will influence both per-sample summary statistics and cross-sample comparisons. We suggest that the microbiome research community pay attention to potentially misleading biological conclusions arising from this issue when benchmarking metagenomic profilers, by carefully considering the type of abundance data that were analyzed and interpreted and clearly stating the strategy used for metagenomic profiling. <<<
翻译
625.
周周复始 (2023-04-30 23:12):
#paper doi: https://doi.org/10.48550/arXiv.2112.05149.DiffuseMorph: Unsupervised Deformable Image Registration Using Diffusion Model.可形变图像配准是医学成像中的基本任务之一。经典的配准算法通常需要较高的计算代价来进行迭代优化。虽然基于深度学习的方法进行快速图像配准已经发展起来,但要获得从移动图像到固定图像较少拓扑折叠的真实连续形变问题仍然具有挑战性。为了解决这个问题,本文提出了一种新的基于扩散模型的图像配准方法,称为DiffuseMorph。DiffuseMorph不仅通过逆扩散过程生成合成的变形图像,并且通过形变场进行图像配准。具体来说,形变场由移动图像和固定图像之间形变的条件分数函数生成。所以可以通过简单地缩放分数的潜在特征,对连续形变进行配准。2D面部和3D医学图像配准任务的实验结果表明,本文方法提供了灵活的形变和拓扑保持能力。
Abstract:
Deformable image registration is one of the fundamental tasks in medical imaging. Classical registration algorithms usually require a high computational cost for iterative optimizations. Although deep-learning-based methods have been developed … >>>
Deformable image registration is one of the fundamental tasks in medical imaging. Classical registration algorithms usually require a high computational cost for iterative optimizations. Although deep-learning-based methods have been developed for fast image registration, it is still challenging to obtain realistic continuous deformations from a moving image to a fixed image with less topological folding problem. To address this, here we present a novel diffusion-model-based image registration method, called DiffuseMorph. DiffuseMorph not only generates synthetic deformed images through reverse diffusion but also allows image registration by deformation fields. Specifically, the deformation fields are generated by the conditional score function of the deformation between the moving and fixed images, so that the registration can be performed from continuous deformation by simply scaling the latent feature of the score. Experimental results on 2D facial and 3D medical image registration tasks demonstrate that our method provides flexible deformations with topology preservation capability. <<<
翻译
626.
cellsarts (2023-04-30 23:11):
#paper SignalP 6.0使用蛋白质语言模型预测所有五种类型的信号肽https://doi.org/10.1038/s41587-021-01156-3 信号肽(SPs)是控制所有生物体中蛋白质分泌和转运的短氨基酸序列。SPs可以从序列数据中预测,但现有算法无法检测到所有已知类型的SPs。我们介绍了SignalP 6.0,这是一个机器学习模型,可以检测所有五种SP类型,并适用于宏基因组数据。SPs是一种短的n端氨基酸序列,在真核生物中将蛋白定向到分泌(Sec)途径,并在原核生物中跨血浆(内)膜进行转运。由于SPs的综合实验鉴定是不现实的,因此SPs的计算预测与细胞生物学的研究具有很高的相关性。SP预测工具能够识别遵循一般分泌或双精氨酸易位(Tat)途径的蛋白质,并预测信号肽酶(SPase)在序列中切割sp2,3的位置。SignalP 5.0能够预测SPase I (Sec/SPI)或SPase II (Sec/ SPII,原核脂蛋白)切割的Sec底物和SPase I (Tat/SPI)切割的Tat底物4。然而,由于缺乏注释数据,SignalP 5.0无法检测由SPase II切割的Tat底物或由SPase III (prepilin peptide ase,有时称为SPase IV2)加工的Sec底物。此类Sec/SPIII SPs控制IV型匹林样蛋白的易位,而IV型匹林样蛋白在原核生物的粘附、运动和DNA摄取中起关键作用。此外,SignalP 5.0对SP结构是不可知的,因为它不能定义构成SP生物学功能的子区(n端n区、疏水h区和c端c区)。 在这里,我们提出了基于蛋白质语言模型(LMs) 6-9的SignalP 6.0,该模型使用了来自生命所有领域数百万未注释的蛋白质序列的信息。LMs创建捕获其生物特性和结构的蛋白质的语义表示。使用这些蛋白质表示,SignalP 6.0可以预测以前版本无法检测到的其他类型的SPs,同时更好地推断与用于创建模型的蛋白质和来源未知的宏基因组数据有远亲性的蛋白质。此外,它还能够确定SPs的分区域.
IF:33.100Q1 Nature biotechnology, 2022-07. DOI: 10.1038/s41587-021-01156-3 PMID: 34980915
Abstract:
Signal peptides (SPs) are short amino acid sequences that control protein secretion and translocation in all living organisms. SPs can be predicted from sequence data, but existing algorithms are unable … >>>
Signal peptides (SPs) are short amino acid sequences that control protein secretion and translocation in all living organisms. SPs can be predicted from sequence data, but existing algorithms are unable to detect all known types of SPs. We introduce SignalP 6.0, a machine learning model that detects all five SP types and is applicable to metagenomic data. <<<
翻译
627.
小小小小小小萌 (2023-04-30 22:02):
#paper doi: 10.1016/j.ccell.2023.03.008 Neutrophil extracellular traps formed during chemotherapy confer treatment resistance via TGF-b activation. 该研究对小鼠静脉注射乳腺癌细胞并待其转移7天,用化疗药物处理后,检测比较了肺部的细胞变化情况,结果发现对于肿瘤肺转移小鼠,化疗会导致肺部中性粒细胞增多。当用Ly6G抗体清除掉中性粒细胞之后,化疗效果又得到了显著增强。血液中的中性粒细胞通过形成NET以杀害微生物,该研究还表明了抑制NET可以增强化疗效果。最后解释了化疗促进NET 形成的生物学机制。
IF:48.800Q1 Cancer cell, 2023-04-10. DOI: 10.1016/j.ccell.2023.03.008 PMID: 37037615
Abstract:
Metastasis is the major cause of cancer death, and the development of therapy resistance is common. The tumor microenvironment can confer chemotherapy resistance (chemoresistance), but little is known about how … >>>
Metastasis is the major cause of cancer death, and the development of therapy resistance is common. The tumor microenvironment can confer chemotherapy resistance (chemoresistance), but little is known about how specific host cells influence therapy outcome. We show that chemotherapy induces neutrophil recruitment and neutrophil extracellular trap (NET) formation, which reduces therapy response in mouse models of breast cancer lung metastasis. We reveal that chemotherapy-treated cancer cells secrete IL-1β, which in turn triggers NET formation. Two NET-associated proteins are required to induce chemoresistance: integrin-αvβ1, which traps latent TGF-β, and matrix metalloproteinase 9, which cleaves and activates the trapped latent TGF-β. TGF-β activation causes cancer cells to undergo epithelial-to-mesenchymal transition and correlates with chemoresistance. Our work demonstrates that NETs regulate the activities of neighboring cells by trapping and activating cytokines and suggests that chemoresistance in the metastatic setting can be reduced or prevented by targeting the IL-1β-NET-TGF-β axis. <<<
翻译
628.
哪有情可长 (2023-04-30 21:56):
#paper reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat doi: doi.org/10.1038/s41586-023-06023-6. 通过多年的大规模田间表型调查和遗传学研究,鉴定到一个能够提升小麦群体产量的关键位点,其中该位点跟已知的Rht基因处于相同的区段,通过构建小麦该区段内三个基因的突变体,发现该基因跟绿色革命基因相比,能够保持半矮杆株型,且茎秆强度、植株耐密性、收获指数、千粒重和产量均有显著的提升。该基因可以是近现代小麦品种后期育种增产的目标基因,对于小麦产量提升具有重要的作用。同时该研究历时10年,通过正向遗传鉴定基因后,对于小麦中的明星基因如何处理也是一个很好的典范,同时也给我分析小麦数据提供了思路,GWAS结果中一些定位到明星基因的位点还可以看下SVN等情况。
IF:50.500Q1 Nature, 2023-05. DOI: 10.1038/s41586-023-06023-6 PMID: 37100915
Abstract:
Modern green revolution varieties of wheat (Triticum aestivum L.) confer semi-dwarf and lodging-resistant plant architecture owing to the Reduced height-B1b (Rht-B1b) and Rht-D1b alleles. However, both Rht-B1b and Rht-D1b are … >>>
Modern green revolution varieties of wheat (Triticum aestivum L.) confer semi-dwarf and lodging-resistant plant architecture owing to the Reduced height-B1b (Rht-B1b) and Rht-D1b alleles. However, both Rht-B1b and Rht-D1b are gain-of-function mutant alleles encoding gibberellin signalling repressors that stably repress plant growth and negatively affect nitrogen-use efficiency and grain filling. Therefore, the green revolution varieties of wheat harbouring Rht-B1b or Rht-D1b usually produce smaller grain and require higher nitrogen fertilizer inputs to maintain their grain yields. Here we describe a strategy to design semi-dwarf wheat varieties without the need for Rht-B1b or Rht-D1b alleles. We discovered that absence of Rht-B1 and ZnF-B (encoding a RING-type E3 ligase) through a natural deletion of a haploblock of about 500 kilobases shaped semi-dwarf plants with more compact plant architecture and substantially improved grain yield (up to 15.2%) in field trials. Further genetic analysis confirmed that the deletion of ZnF-B induced the semi-dwarf trait in the absence of the Rht-B1b and Rht-D1b alleles through attenuating brassinosteroid (BR) perception. ZnF acts as a BR signalling activator to facilitate proteasomal destruction of the BR signalling repressor BRI1 kinase inhibitor 1 (TaBKI1), and loss of ZnF stabilizes TaBKI1 to block BR signalling transduction. Our findings not only identified a pivotal BR signalling modulator but also provided a creative strategy to design high-yield semi-dwarf wheat varieties by manipulating the BR signal pathway to sustain wheat production. <<<
翻译
629.
张贝 (2023-04-30 21:54):
#paper Detecting Liver Cancer Using Cell-Free DNA Fragmentomes Cancer Discov. 023 Mar 1;13(3):616-631. doi: 10.1158/2159-8290.CD-22-0659. 本文是DELFI技术应用于肝癌筛查的最新研究成果。DELFI的全称是DNA evaluation of fragments for early interception,即利用血液cfDNA全基因组片段化特征间的差异,来区分肿瘤患者和非癌症受试者。本文构建了两个机器学习模型,分别适用于肝癌高危人群和低风险人群,这两个模型纳入的特征类型略有差异,在低风险人群模型中新增TFBS特征。最后,作者使用蒙特卡洛模拟评估DELFI模型在10万理论高危人群中的性能,与指南推荐的腹部超声联合AFP的检测方法相比,DELFI技术不仅极大提高肝癌的检出率,同时有望降低肝癌检测的假阴性率。
Abstract:
Abstract Liver cancer is a major cause of cancer mortality worldwide. Screening individuals at high-risk, including those with cirrhosis and viral hepatitis, provides an avenue for improved survival, but current … >>>
Abstract Liver cancer is a major cause of cancer mortality worldwide. Screening individuals at high-risk, including those with cirrhosis and viral hepatitis, provides an avenue for improved survival, but current screening methods are inadequate. In this study, we used whole-genome cell-free DNA fragmentome analyses to evaluate 724 individuals from the US, EU, or Hong Kong with hepatocellular carcinoma (HCC) or who were at average or high-risk for HCC. Using a machine learning model that incorporated multi-feature fragmentome data, the sensitivity for detecting cancer was 88% in an average risk population at 98% specificity, and 85% among high-risk individuals at 80% specificity. We validated these results in an independent population. cfDNA fragmentation changes reflected genomic and chromatin changes in liver cancer, including from transcription factor binding sites. These findings provide a biological basis for changes in cfDNA fragmentation in patients with liver cancer and provide an accessible approach for non-invasive cancer detection. <<<
翻译
630.
大勇 (2023-04-30 21:33):
#paper Tim-4+ cavity-resident macrophages impair anti-tumor CD8+ T cell immunity. Cancer Cell. 2021 Jul 12;39(7):973-988.e9. doi: 10.1016/j.ccell.2021.05.006. Epub 2021 Jun 10. PMID: 34115989; PMCID: PMC9115604.文章的概念比较新颖,探究了腹腔转移肿瘤对免疫检查点抑制剂耐药的相关机制,找到了体腔驻留的TIM4阳性的巨噬细胞在其中发挥的影响,利用TIM4阳性的巨噬细胞与CD8+CD39+T细胞的相互作用,阐释了体腔驻留巨噬细胞可以抑制CD8T细胞的功能,并且TIM4单抗可以与PD1单抗联合用药治疗肿瘤,提高疗效。
IF:48.800Q1 Cancer cell, 2021-07-12. DOI: 10.1016/j.ccell.2021.05.006 PMID: 34115989
Abstract:
Immune checkpoint blockade (ICB) has been a remarkable clinical advance for cancer; however, the majority of patients do not respond to ICB therapy. We show that metastatic disease in the … >>>
Immune checkpoint blockade (ICB) has been a remarkable clinical advance for cancer; however, the majority of patients do not respond to ICB therapy. We show that metastatic disease in the pleural and peritoneal cavities is associated with poor clinical outcomes after ICB therapy. Cavity-resident macrophages express high levels of Tim-4, a receptor for phosphatidylserine (PS), and this is associated with reduced numbers of CD8 T cells with tumor-reactive features in pleural effusions and peritoneal ascites from patients with cancer. We mechanistically demonstrate that viable and cytotoxic anti-tumor CD8 T cells upregulate PS and this renders them susceptible to sequestration away from tumor targets and proliferation suppression by Tim-4 macrophages. Tim-4 blockade abrogates this sequestration and proliferation suppression and enhances anti-tumor efficacy in models of anti-PD-1 therapy and adoptive T cell therapy in mice. Thus, Tim-4 cavity-resident macrophages limit the efficacy of immunotherapies in these microenvironments. <<<
翻译
631.
庞庞 (2023-04-30 19:59):
#paper doi:  10.1002/hbm.25985 Prediction of childhood maltreatment and subtypes with personalized functional connectome of large-scale brain networks 童年虐待 (CM) 对儿童的身心健康有着长期的影响。 然而,CM 的神经基础仍不清楚。本研究基于个体化的功能脑网络分区方法,计算功能网络连接 (FNC)和通过儿童创伤问卷 (CTQ) 评估的 CM 影响之间的关联。个体化的 FNC可以很好地预测 CM 总分和子量表分数,涉及到了默认模式网络、额顶叶网络、视觉网络、边缘网络、运动网络、背侧和腹侧注意网络,不同的网络对预测有不同的贡献。
IF:3.500Q1 Human brain mapping, 2022-10-15. DOI: 10.1002/hbm.25985 PMID: 35735128
Abstract:
Childhood maltreatment (CM) has a long impact on physical and mental health of children. However, the neural underpinnings of CM are still unclear. In this study, we aimed to establish … >>>
Childhood maltreatment (CM) has a long impact on physical and mental health of children. However, the neural underpinnings of CM are still unclear. In this study, we aimed to establish the associations between functional connectome of large-scale brain networks and influences of CM evaluated through Childhood Trauma Questionnaire (CTQ) at the individual level based on resting-state functional magnetic resonance imaging data of 215 adults. A novel individual functional mapping approach was employed to identify subject-specific functional networks and functional network connectivities (FNCs). A connectome-based predictive modeling (CPM) was used to estimate CM total and subscale scores using individual FNCs. The CPM established with FNCs can well predict CM total scores and subscale scores including emotion abuse, emotion neglect, physical abuse, physical neglect, and sexual abuse. These FNCs primarily involve default mode network, fronto-parietal network, visual network, limbic network, motor network, dorsal and ventral attention networks, and different networks have distinct contributions to predicting CM and subtypes. Moreover, we found that CM showed age and sex effects on individual functional connections. Taken together, the present findings revealed that different types of CM are associated with different atypical neural networks which provide new clues to understand the neurobiological consequences of childhood adversity. <<<
翻译
632.
Spring (2023-04-30 18:46):
#paperdoi: 10.1016/j.advnut.2023.04.004 Tolerable upper intake level (UL) for individual amino acids in humans: A narrative review of recent clinical studies ① 总结6种必需氨基酸、2种非必需氨基酸、2种非蛋白质源性氨基酸的摄入量研究;② 年轻人及老年人的亮氨酸UL分别为每天35克及30克,色氨酸的UL为每天4.5克;③ 甲硫氨酸的NOAEL及LOAEL分别为每天3.2克及6.4克;④ 精氨酸的NOAEL为每天30克,赖氨酸的NOAEL及LOAEL分别为每天6克及7.5克;⑤ 组氨酸的NOAEL及LOAEL分别为每天8克及12克;⑥ 苯丙氨酸、丝氨酸、鸟氨酸、瓜氨酸的NOAEL分别为每天12克、12克、12克、24克。
Abstract:
Individual amino acids are widely popular as supplements because of various perceived and real health benefits. However, currently, there are no recommendations set by national health agencies for tolerable upper … >>>
Individual amino acids are widely popular as supplements because of various perceived and real health benefits. However, currently, there are no recommendations set by national health agencies for tolerable upper intake levels (UL) for amino acids because of a lack of well-conducted human dose-response trials. In the past decade, under the initiative of the International Council on Amino Acid Science, a nonprofit organization, a series of UL human clinical studies were conducted. The goal of this narrative review is to summarize the studies on 6 essential amino acids (leucine, tryptophan, methionine, lysine, histidine, and phenylalanine), 2 nonessential amino acids (arginine and serine), and 2 nonproteinogenic amino acids (ornithine and citrulline) and provide the first set of ULs. A brief background of the concept of the DRI framework of UL, the concept of UL for amino acids, and a perspective of the results are also provided. The data suggest that in relatively healthy adult individuals, the tested amino acids are well tolerated, and ULs, or the no-observed-adverse-effect-level (NOAEL), lowest-observed-adverse-effect-level (LOAEL), can be determined. The ULs were for leucine-young (35 g/d), tryptophan (4.5 g/d), and leucine-elderly (30 g/d); NOAEL and LOAEL for methionine at 3.2 and 6.4 g/d, respectively; NOAEL for arginine (30 g/d); NOAEL and LOAEL for lysine at 6 and 7.5 g/d, respectively; NOAEL and LOAEL for histidine at 8 and 12 g/d, respectively; and NOAEL for phenylalanine (12 g/d), serine (12 g/d), ornithine (12 g/d) and citrulline (24 g/d). This first set of human UL data are hoped to help national and international agencies set safety standards for supplemental amino acids. <<<
翻译
633.
符毓 (2023-04-30 17:23):
#paper doi: 10.1126/science.272.5258.85 Science, 1996, Imprint Lithography with 25-Nanometer Resolution。Stephen Chou(周郁)首次提出纳米压印方式用半导体集成电路生产上,并成功做出25nm的精度。纳米压印比起光刻具有低成本,高通量的优势。 相关行业最新进展:近年来佳能已将纳米压印应用到了 15nm 的 NAND 闪存制造上,并宣称2025年有望用此技术量产5nm芯片
IF:44.700Q1 Science, 2006. DOI: 10.1126/science.272.5258.85
Abstract:
A high-throughput lithographic method with 25-nanometer resolution and smooth vertical sidewalls is proposed and demonstrated. The technique uses compression molding to create a thickness contrast pattern in a thin resist … >>>
A high-throughput lithographic method with 25-nanometer resolution and smooth vertical sidewalls is proposed and demonstrated. The technique uses compression molding to create a thickness contrast pattern in a thin resist film carried on a substrate, followed by anisotropic etching to transfer the pattern through the entire resist thickness. Metal patterns with a feature size of 25 nanometers and a period of 70 nanometers were fabricated with the use of resist templates created by imprint lithography in combination with a lift-off process. With further development, imprint lithography should allow fabrication of sub-10-nanometer structures and may become a commercially viable technique for manufacturing integrated circuits and other nanodevices. <<<
翻译
634.
Vincent (2023-04-30 15:13):
#paper doi: https://www.nature.com/articles/s41576-023-00586-w Best practices for single-cell analysis across modalities. Nature review genetics,2023. 这篇综述文章来自Fabian Theis组, 是一篇极好的单细胞分析指导文章。文章涵盖了几种不同的技术(scRNA-seq, scATAC-seq, scTCR/BCR, spatial transcriptomics), 对于每一种技术路线,介绍了完整的分析流程和目前最好的处理方法,例如scRNA, 介绍了原始数据处理、数据过滤和去杂,标准化和批次效应去除,降维聚类分型,拟时序分析和RNA速率分析,差异基因分析,细胞组成分析和细胞通讯分析等等。对于每一个步骤,文章会总结当前的最佳实践(如果有其他文章做过基准测试)或者给出分析建议(如果目前还没有基准测试的工作)。鉴于当前单细胞分析领域各种方法层出不穷,这篇文章提供了一个很好的指导总结,非常推荐做单细胞分析的朋友阅读。
Abstract:
Recent advances in single-cell technologies have enabled high-throughput molecular profiling of cells across modalities and locations. Single-cell transcriptomics data can now be complemented by chromatin accessibility, surface protein expression, adaptive … >>>
Recent advances in single-cell technologies have enabled high-throughput molecular profiling of cells across modalities and locations. Single-cell transcriptomics data can now be complemented by chromatin accessibility, surface protein expression, adaptive immune receptor repertoire profiling and spatial information. The increasing availability of single-cell data across modalities has motivated the development of novel computational methods to help analysts derive biological insights. As the field grows, it becomes increasingly difficult to navigate the vast landscape of tools and analysis steps. Here, we summarize independent benchmarking studies of unimodal and multimodal single-cell analysis across modalities to suggest comprehensive best-practice workflows for the most common analysis steps. Where independent benchmarks are not available, we review and contrast popular methods. Our article serves as an entry point for novices in the field of single-cell (multi-)omic analysis and guides advanced users to the most recent best practices. <<<
翻译
635.
尹志 (2023-04-30 10:32):
#paper Broadly applicable and accurate protein design by integrating structure prediction networks and diffusion generative models doi: https://doi.org/10.1101/2022.12.09.519842 这篇文章提出了一种全新的蛋白质设计方法,叫做rf diffusion,它使用深度生成学习生成全新的蛋白质结构。文章主要使用的是 diffusion model,考虑到蛋白质骨架的复杂几何性质以及氨基酸序列-结构的复杂关系,蛋白质生成任务一直以来的挑战很大。这篇工作 使用diffusion model的思路如下:1.使用RoseTTAFold作为去噪网络,考虑到RoseTTA本来就是baker组用来做蛋白质设计的(更多的是基于物理的),这个去噪网络的选择还是很巧妙的;2.整个加噪去噪过程主要针对alpha碳原子的坐标进行,因此rf diffusion的思路是先对骨架结构进行生成的;3.然后full 的protein structure是通过backbone tracking的技术来实现的,这个过程可以理解为基于一些几何约束、bond的长度角度参数等等为已经预测的alpha碳原子添加缺失的bond和原子,4.侧链是通过rotamer实现的,rotamer是一个已经对 每个氨基酸残基做了预先计算的库,它可以为你选择符合能量最优的构象的侧链结构。 因此整个蛋白质生成的过程可以认为是深度生成模型+物理约束+后处理(预先计算)来实现的。当然,这篇工作也做了很多的实验对设计进行验证。baker组在之后使用了rfdiffusion做了后续的一些设计工作,包括De novo design of high-affinity protein binders to bioactive helical peptides这个工作,并在不久前开源了rf diffusion的代码,也有很多蛋白质设计的研究人员开始大量尝试 基于rfdiffusion的设计,并尝试进行湿实验的验证,因此这绝对是一篇开创性的工作,值得各位小伙伴关注。
Abstract:
AbstractThere has been considerable recent progress in designing new proteins using deep learning methods1–9. Despite this progress, a general deep learning framework for protein design that enables solution of a … >>>
AbstractThere has been considerable recent progress in designing new proteins using deep learning methods1–9. Despite this progress, a general deep learning framework for protein design that enables solution of a wide range of design challenges, includingde novobinder design and design of higher order symmetric architectures, has yet to be described. Diffusion models10,11have had considerable success in image and language generative modeling but limited success when applied to protein modeling, likely due to the complexity of protein backbone geometry and sequence-structure relationships. Here we show that by fine tuning the RoseTTAFold structure prediction network on protein structure denoising tasks, we obtain a generative model of protein backbones that achieves outstanding performance on unconditional and topology-constrained protein monomer design, protein binder design, symmetric oligomer design, enzyme active site scaffolding, and symmetric motif scaffolding for therapeutic and metal-binding protein design. We demonstrate the power and generality of the method, called RoseTTAFold Diffusion (RFdiffusion), by experimentally characterizing the structures and functions of hundreds of new designs. In a manner analogous to networks which produce images from user-specified inputs, RFdiffusionenables the design of diverse, complex, functional proteins from simple molecular specifications. <<<
翻译
636.
颜林林 (2023-04-30 10:31):
#paper doi:10.1109/TNB.2023.3254514 IEEE transactions on nanobioscience, 2023, RBS: A Rotational Coding Based on Blocking Strategy for DNA Storage. 利用DNA作为介质研发数据存储方案,是近几年的热点之一,许多研究所和公司都竞相开展,但投入和进展却层次不齐。这也是我个人比较感兴趣的方向之一,因此关注到最近刚发表出来的这篇文章,顺便点评一下。虽然这篇文章并不算多出彩,也没有什么重大突破,但它是一篇纯算法的概念验证工作,不涉及到分子实验,倒是比较适合我这种业余感兴趣者效仿。用DNA介质存储数据,面临各种现实问题,比如GC含量需要限制在一定范围,过高或过低的GC含量,都会在合成和测序上导致问题,再比如不能有连续重复片段等。也因此,对数据进行DNA字母的编码,不能简单随便设置某种一一对应规则,而需要同时考虑各类分子特性限制。本文提出了一种数据编解码算法RBS,并使用文本、图片数据测试,评估诸如GC含量、重复片段数量、汉明距离、自由能等,以确认该算法用于DNA存储的可行性和效率。
Abstract:
The data volume of global information has grown exponentially in recent years, but the development of silicon-based memory has entered a bottleneck period. Deoxyribonucleic acid (DNA) storage is drawing attention … >>>
The data volume of global information has grown exponentially in recent years, but the development of silicon-based memory has entered a bottleneck period. Deoxyribonucleic acid (DNA) storage is drawing attention owing to its advantages of high storage density, long storage time, and easy maintenance. However, the base utilization and information density of existing DNA storage methods are insufficient. Therefore, this study proposes a rotational coding based on blocking strategy (RBS) for encoding digital information such as text and images in DNA data storage. This strategy satisfies multiple constraints and produces low error rates in synthesis and sequencing. To illustrate the superiority of the proposed strategy, it was compared and analyzed with existing strategies in terms of entropy value change, free energy size, and Hamming distance. The experimental results show that the proposed strategy has higher information storage density and better coding quality in DNA storage, so it will improve the efficiency, practicality, and stability of DNA storage. <<<
翻译
637.
白鸟 (2023-04-29 23:16):
#paper Single-cell transcriptomics dissects hematopoietic cell destruction and T-cell engagement in aplastic anemia. Blood. 2021. 研究背景:再生障碍性贫血 (AA) 是一种T细胞介导的造血系统自身免疫性疾病,表现为造血干细胞和祖细胞 (HSPC) 的严重耗竭。异常活化的T淋巴细胞攻击自身造血干/祖细胞(HSPC)是再生障碍性贫血(AA)发病重要的机制。 研究难点:受限于技术和 HSPC 在骨髓衰竭背景下的稀疏性。AA患者骨髓残留HSPC细胞数量极少,精细剖析骨髓损伤后HSPC各组分的病理变化及T淋巴细胞免疫打击HSPC的分子机制比较困难。 样本类型:健康供体(healthy donors,n = 8)+ 非重度再生障碍性贫血患者 (non-SAA, n = 19) + 重度再生障碍性贫血患者 (SAA,  n = 4 );另加 药物处理组:免疫抑制治疗(IST)后患者 样本取样:骨髓及外周血中分选出CD34+造血干/祖细胞和CD4+/CD8+ T淋巴细胞 实验技术:STRT-Seq(高测序深度) + Smart-seq2 研究思路:不同疾病/健康组 -> 流式分选细胞 - > CD34+造血干/祖细胞和CD4+/CD8+ T淋巴细胞->单细胞测序(STRT-Seq + Smart-seq2)->定义了9类HSPC细胞亚群->基因表达和转录调控网络分析 研究结果: ① STRT-seq克服骨髓残留造血干细胞和祖细胞HSPC数量不足的限制,对AA患者的HSPC和T细胞进行分析,分别获得了2,385个HSPC和4,081个CD4+/CD8+ T细胞的单细胞转录组,定义了9类HSPCs细胞亚群,首次绘制了AA血液病理图谱,揭示了AA发病,特别是恶性转化的新机制。 ② AA中残留的HSPC在基因表达和转录调控网络中表现出谱系特异性的改变,提示存在谱系选择性造血损伤。 ③ 综合分析HSPC和T细胞的基因表达,确定了细胞类型特异性配体-受体相互作用是AA中免疫攻击的关键分子介质。 ④ 通过追踪免疫抑制治疗(IST)后的患者,发现HSPCs和T淋巴细胞的基因表达没有完全恢复到正常水平,甚至接近治疗前的状态,这可能是AA患者需要长期维持免疫抑制治疗的主要原因之一。
IF:21.000Q1 Blood, 2021-07-08. DOI: 10.1182/blood.2020008966 PMID: 33763704
Abstract:
Aplastic anemia (AA) is a T cell-mediated autoimmune disorder of the hematopoietic system manifested by severe depletion of the hematopoietic stem and progenitor cells (HSPCs). Nonetheless, our understanding of the … >>>
Aplastic anemia (AA) is a T cell-mediated autoimmune disorder of the hematopoietic system manifested by severe depletion of the hematopoietic stem and progenitor cells (HSPCs). Nonetheless, our understanding of the complex relationship between HSPCs and T cells is still obscure, mainly limited by techniques and the sparsity of HSPCs in the context of bone marrow failure. Here we performed single-cell transcriptome analysis of residual HSPCs and T cells to identify the molecular players from patients with AA. We observed that residual HSPCs in AA exhibited lineage-specific alterations in gene expression and transcriptional regulatory networks, indicating a selective disruption of distinct lineage-committed progenitor pools. In particular, HSPCs displayed frequently altered alternative splicing events and skewed patterns of polyadenylation in transcripts related to DNA damage and repair, suggesting a likely role in AA progression to myelodysplastic syndromes. We further identified cell type-specific ligand-receptor interactions as potential mediators for ongoing HSPCs destruction by T cells. By tracking patients after immunosuppressive therapy (IST), we showed that hematopoiesis remission was incomplete accompanied by IST insensitive interactions between HSPCs and T cells as well as sustained abnormal transcription state. These data collectively constitute the transcriptomic landscape of disrupted hematopoiesis in AA at single-cell resolution, providing new insights into the molecular interactions of engaged T cells with residual HSPCs and render novel therapeutic opportunities for AA. <<<
翻译
638.
小年 (2023-04-29 17:35):
#paper DOI: 10.1182/blood.2020006287. Tumor-intrinsic and -extrinsic determinants of response to blinatumomab in adults with B-ALL. Blood. 2021 Jan 28;137(4):471-484. 通过对肿瘤和免疫细胞的综合基因组分析,证明肿瘤内在和外在因素都会影响患者对blinatumomab(博纳吐单抗治疗)的反应。 单细胞测序研究了44位采用blinatumomab治疗的复发性/难治性B-ALL成人患者(包括2例MRD阳性的患者)。 血液病患者的总体缓解率为 55%,CRLF2 重排费城染色体样 ALL 患者(Ph样ALL)的缓解率很高(12 [75%] of 16)。 转录组结果来看,应答者的预处理样本在肿瘤内表现出免疫应答增强。在治疗期间,外显子CD19 ex2part的外显子剪接亚型的表达增加与治疗失败有关。 未来的研究可评估使用ex2part作为CD19定向免疫疗法(包括blinatumomab和CAR19)反应的生物标志物。
IF:21.000Q1 Blood, 2021-01-28. DOI: 10.1182/blood.2020006287 PMID: 32881995
Abstract:
Blinatumomab, a bispecific antibody that directs CD3+ T cells to CD19+ tumor cells, shows variable efficacy in B-progenitor acute lymphoblastic leukemia (B-ALL). To determine tumor-intrinsic and -extrinsic determinants of response, … >>>
Blinatumomab, a bispecific antibody that directs CD3+ T cells to CD19+ tumor cells, shows variable efficacy in B-progenitor acute lymphoblastic leukemia (B-ALL). To determine tumor-intrinsic and -extrinsic determinants of response, we studied 44 adults with relapsed or refractory B-ALL (including 2 minimal residual disease positive) treated with blinatumomab using bulk tumor and single-cell sequencing. The overall response rate in patients with hematological disease was 55%, with a high response rate in those with CRLF2-rearranged Philadelphia chromosome-like ALL (12 [75%] of 16). Pretreatment samples of responders exhibited a tumor-intrinsic transcriptomic signature of heightened immune response. Multiple mechanisms resulted in loss of CD19 expression, including CD19 mutations, CD19-mutant allele-specific expression, low CD19 RNA expression, and mutations in CD19 signaling complex member CD81. Patients with low hypodiploid ALL were prone to CD19- relapse resulting from aneuploidy-mediated loss of the nonmutated CD19 allele. Increased expression of a CD19 isoform with intraexonic splicing of exon 2, CD19 ex2part, at baseline or during therapy was associated with treatment failure. These analyses demonstrate both tumor-intrinsic and -extrinsic factors influence blinatumomab response. We show that CD19 mutations are commonly detected in CD19- relapse during blinatumomab treatment. Identification of the CD19 ex2part splice variant represents a new biomarker predictive of blinatumomab therapy failure. <<<
翻译
639.
李翛然 (2023-04-28 17:37):
#paper De novo design of protein interactions with lerned surface pingerprints doi: 10.1038/s41586-023-05993-x. 文章的主要思路是分为三个阶段:(1)使用MaSIF-site预测目标蛋白质表面上具有高结合倾向的埋藏界面位点;(2)使用MaSIF-seed基于表面指纹寻找互补的结构基元(结合种子),这些基元具有与目标位点相匹配的特征;(3)将结合种子移植到蛋白质骨架上,使用Rosetta优化设计界面,增加稳定性和额外的接触。 文章的主要结论是,作者利用这种表面为中心的方法成功地设计并实验验证了针对四种蛋白质靶标的从头结合剂:SARS-CoV-2刺突蛋白、PD-1、PD-L1和CTLA-4。其中一些设计经过实验优化,而另一些则完全在计算机上生成,达到了纳摩尔级别的亲和力。结构和突变分析显示预测非常准确。总体而言,作者的方法能够捕捉分子识别的物理和化学决定因素,为从头设计蛋白质相互作用以及更广泛地设计具有功能的人工蛋白质提供了一种方法. 以上是通过chat GPT总结的。 不过我读完的感受就是,我并不认为这篇文章的水平是 nature 正刊的水平, masif 的算法在蛋白质结构对比上确实有用,但是背后有个深层次的问题这篇文章没有谈到,即目前来说,对于已知蛋白设计一个有效的配体蛋白,算法已经比较丰富了。并且最近2年发的文章已经有很好的实验结果来验证。 但是对于结构全新,或者说没有任何可用配体的蛋白来说,这个挑战非常巨大,文章并没有提到这种问题出现后的解决思路,而且甚至算法的创新比不上前段时间的 baker 的 rf diffusion. 总之吧 现在真的是蓝海市场。 这个领域机会太多了
IF:50.500Q1 Nature, 2023-05. DOI: 10.1038/s41586-023-05993-x PMID: 37100904
Abstract:
Physical interactions between proteins are essential for most biological processes governing life. However, the molecular determinants of such interactions have been challenging to understand, even as genomic, proteomic and structural … >>>
Physical interactions between proteins are essential for most biological processes governing life. However, the molecular determinants of such interactions have been challenging to understand, even as genomic, proteomic and structural data increase. This knowledge gap has been a major obstacle for the comprehensive understanding of cellular protein-protein interaction networks and for the de novo design of protein binders that are crucial for synthetic biology and translational applications. Here we use a geometric deep-learning framework operating on protein surfaces that generates fingerprints to describe geometric and chemical features that are critical to drive protein-protein interactions. We hypothesized that these fingerprints capture the key aspects of molecular recognition that represent a new paradigm in the computational design of novel protein interactions. As a proof of principle, we computationally designed several de novo protein binders to engage four protein targets: SARS-CoV-2 spike, PD-1, PD-L1 and CTLA-4. Several designs were experimentally optimized, whereas others were generated purely in silico, reaching nanomolar affinity with structural and mutational characterization showing highly accurate predictions. Overall, our surface-centric approach captures the physical and chemical determinants of molecular recognition, enabling an approach for the de novo design of protein interactions and, more broadly, of artificial proteins with function. <<<
翻译
640.
钟鸣 (2023-04-28 17:27):
#paper doi:10.3390/ph16030328 Chemical Composition and Antimicrobial Potential of a Plant-Based Substance for the Treatment of Seborrheic Dermatitis 脂溢性皮炎 (SD) 是最常见的头皮皮肤病,全世界高达 50% 的成年人都会发生。SD的发生与多种因素有关,但确切的发病机制仍未阐明。目前对SD的治疗以抗真菌和抗炎为主,但长期使用这类药物不仅有引起副作用的风险,真菌耐药性的诱导也是不可忽视的风险之一,为此作者将目光投向了植物源药物,即本文中的互叶白千层叶油(TTO)。作者首先使用GS/MS分析了成分,确定了 TTO 特有的 10 种抗菌单萜和倍半萜以及以前未在 TTO 中检测到的 7 种新萜。随后的抑菌试验和抗真菌实验表明这种物质的抗菌活性与苯扎氯铵、酮康唑和氯咪唑相当;因此,该物质有望用于进一步研究和评估可能用于治疗 SD 的药物开发。
Abstract:
Seborrheic dermatitis (SD) is the most prevalent dermatological disease, occurring in up to 50% of newborns, children, and adults around the world. The antibacterial and antifungal resistance contributed to the … >>>
Seborrheic dermatitis (SD) is the most prevalent dermatological disease, occurring in up to 50% of newborns, children, and adults around the world. The antibacterial and antifungal resistance contributed to the search for new natural substances and the development of a novel substance based on () leaf oil (TTO), 1,8-cineole (eucalyptol), and α-(-)-bisabolol. Thus, this work aimed to determine the chemical composition of the novel plant-based substance and to evaluate its antimicrobial activity against standard microorganisms involved in the pathogenesis of SD. Moreover, the chemical composition of the substance was analyzed by gas chromatography coupled with mass spectrometry (GC/MS). (), (), (), and () were used for antimicrobial and antifungal assays by means of the broth microdilution method to determine the minimal inhibitory concentration (MIC). Finally, the substance's ability to inhibit () was evaluated. Eighteen compounds from different chemical groups were identified by GC/MS. The major biologically active compounds of the substance were terpinen-4-ol (20.88%), 1,8-cineole (22.28%), (-)-α-bisabolol (25.73%), and o-cymene (8.16%). The results showed that the substance has a synergistic antimicrobial and antifungal activity, while and strains were the most susceptible. Furthermore, the substance inhibited , which is a main pathogen involved in the pathogenesis of SD and clinical manifestations. It can be concluded that the novel plant-based substance has a promising potential against and scalp commensal bacteria and may be helpful for the development of new drugs for treatment of dandruff and SD. <<<
翻译
回到顶部