当前共找到 1 篇文献分享。
1.
龙海晨
(2022-04-10 12:28):
#paper Heat Shock Protein Beta 1 is a Prognostic
Biomarker and Correlated with Immune Infiltrates
in Hepatocellular Carcinoma PMID: 34531676
PMCID: PMC8439715
DOI: 10.2147/IJGM.S330608
推荐理由，最近在尝试自己做生信分析，这是我尝试复现文中生信分析方法的文章，文章使用生信分析和自己做免疫组化验证相结合的方法进行实验。
文章研究目的：研究旨在阐明HSPB1在肝癌中的表达及其潜在的治疗和预后价值。方法：从癌症基因组图谱和基因表达综合数据库中收集了肝癌和正常肝组织中HSPB1表达水平的数据。用免疫组织化学（IHC）对其进行了验证。ROC和Kaplan-Meier 生存曲线研究分析HSPB1在肝癌预后中的作用。生信分析部分包含：RNA-Sequencing Data and Bioinformatics（使用TCGA数据库数据GEO分析，免疫组化实验验证）ROC和Kaplan-Meier 生存曲线分析HSPB1在肝癌患者中的预后。单变量和多变量回归分析，分析HSPB1表达与肝癌生存率之间的相关性。用(STRING) 构建HSPB1的蛋白质相互作用（PPI）网络。HSPB1和HSPB1基因信号通路预测使用Gene Ontology (GO)和Kyoto Encyclopedia of Genes and Genomes (KEGG).用Tumor Immune Estimation Resource (TIMER) 和single sample Gene Set Enrichment Analysis (ssGSEA)分析HSPB1与肿瘤之间的关系。免疫细胞，包括B细胞、中性粒细胞、巨噬细胞、CD4+T细胞和CD8+T细胞。文章结论：HSPB1表达和免疫细胞浸润和肝癌患者预后之间存在显著相关性。HSPB1的表达可能对肝癌患者有明显的预后价值，而且可能是肝癌免疫治疗的潜在靶点。
IF:2.100Q2
International journal of general medicine,
2021.
DOI: 10.2147/IJGM.S330608
PMID: 34531676
PMCID:PMC8439715
Abstract:
BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most serious malignancies. The main features of HCC are vascular invasion and drug resistance. Ferroptosis is a novel cell program that is …
>>>
BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most serious malignancies. The main features of HCC are vascular invasion and drug resistance. Ferroptosis is a novel cell program that is involved in several diseases, such as cancer. Heat shock protein beta 1 (HSPB1) is a major component of heat shock proteins. A recent study showed that HSPB1 could be a new therapeutic target for colorectal cancer with 5-fluorouracil-acquired resistance. However, the functional role of HSPB1 in HCC remains unclear.AIM: The aim of this study is to clarify HSPB1 expression in HCC and its potential therapeutic and prognostic value.METHODS: We collected data on HSPB1 expression levels in HCC and normal liver tissues from The Cancer Genome Atlas and Gene Expression Omnibus databases. We then validated it using immunohistochemistry (IHC). Receiver operating characteristic and Kaplan-Meier survival curves were used to investigate the role of HSPB1 in the prognosis analysis of HCC. Further, we used the online Search Tool for the Retrieval of Interacting Genes/Proteins website, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes to conduct enrichment analysis and identify the predictive signaling pathways. Meanwhile, we used the TIMER and GSVA package of R (v3.6.3) to analyze the association between HSPB1 and immunocyte infiltration.RESULTS: Compared to normal tissues, there was differential expression of HSPB1 in pan-cancers. HSPB1 expression was higher in HCC tissues than in normal tissues (p<0.05). There was an evident significant difference between HSPB1 mRNA levels and histologic grade, vascular invasion, and alpha-fetoprotein level (all p values<0.05). Univariate analysis indicated that HCC patients with high HSPB1 levels had shorter overall survival rates than those with low HSPB1 levels (p<0.05). MAPK14, HSPA8, MAPKAPK3, MAPKAPK5, and MAPKAPK2 are essential proteins that interact with HSPB1. There was a significant correlation between HSPB1 expression levels and immune cell infiltration, including CD4+ T cells (r=0.203, p<0.05).CONCLUSION: High HSPB1 expression is closely associated with a worse prognosis in HCC patients, and HSPB1 may be a target of immunotherapy in HCC.
<<<
翻译