Abstract Detection dogs are required to search for and alert to specific odours of interest, such as drugs, cadavers, disease markers and explosives. However, the odour released from different samples of the same target substance will vary for a number of reasons, including the production method, evaporation, degradation, or by being mixed with extraneous odours. Generalisation, the tendency to respond in the same manner to stimuli which are different – but similar to – a conditioned stimulus, is therefore a crucial requirement for working detection dogs. Odour is a complex modality which poses unique challenges in terms of reliably predicting generalisation, when compared with auditory or visual stimuli. The primary aim of this review is to explore recent advances in our understanding of generalisation and the factors that influence it, and to consider these in light of detection dog training methods currently used in the field. We identify potential risks associated with certain training practices, and highlight areas where research is lacking and which warrant further investigation. <<<