龙海晨
(2023-09-27 10:40):
#paper Falahi S, Zamanian MH, Feizollahi P, Rezaiemanesh A, Salari F, Mahmoudi Z, Gorgin Karaji A. Evaluation of the relationship between IL-6 gene single nucleotide polymorphisms and the severity of COVID-19 in an Iranian population. Cytokine. 2022 Jun;154:155889. doi: 10.1016/j.cyto.2022.155889. Epub 2022 Apr 19. PMID: 35461173; PMCID: PMC9015956. 这是一篇2022年发表的新冠研究的文章。这个杂志影响因子3.8，对于这个杂志我真是感情复杂，当初我2022年投稿该杂志，他们说我不适合在他们这发表，然后给我推荐了另一个杂志，是杂志内部推荐，我的文章自动过去，然后他推荐的那个杂志把我拒稿了。现在，我很感谢他们当初的不发之恩，最后我的文章在4.6的杂志上发表还是中科院的Top期刊。这是一篇阴性结果的研究，估计就因为蹭了当时新冠的热点得以发表。做的实验不多研究的也简单，就是SNP位点的，研究的伊朗克尔曼沙赫库尔德人，共346人（175名重度新冠肺炎患者和171名轻度新冠肺炎患者）分析白细胞介素-6（IL-6）基因多态性，从患者外周血白细胞中提取基因组DNA以确定三个选择的SNPs的基因型（rs1800795（−174 G>C）、rs1800796（−572 G>C，和rs1800797（−597 G>A）），结果表明这些SNP与伊朗克尔曼沙赫库尔德人口中新冠肺炎的严重程度无关。SNP，阴性结果，如果不是当初新冠的热度，估计发不了这么高分的杂志。
Evaluation of the relationship between IL-6 gene single nucleotide polymorphisms and the severity of COVID-19 in an Iranian population
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Abstract:
BACKGROUND: Emerged coronavirus disease 2019 (COVID-19) is a pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-COV-2). Disease severity is associated with elevated levels of proinflammatory cytokines, such as interleukin-6 (IL-6). Genetic polymorphisms in the regulatory regions of cytokine genes may be associated with differential cytokine production in COVID-19 patients. This study aimed to investigate the association between three potentially functional single-nucleotide polymorphisms (SNPs) in the promoter region of IL-6 and the severity of susceptibility to COVID-19 in an Iranian population.METHODS: In total, 346 individuals (175 patients with severe COVID-19 and 171 patients with mild COVID-19) were recruited for this cohort study. Genomic DNA was extracted from peripheral blood leukocytes of patients to determine the genotypes of three selected SNPs (rs1800795 (-174 G > C), rs1800796 (-572 G > C), and rs1800797 (-597 G > A)) in the promoter region of the IL-6 gene using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.RESULTS: There were no significant differences in the genotype or allele distribution of selected SNPs (rs1800795 (-174 G > C), rs1800796 (-572 G > C), and rs1800797 (-597 G > A)) in the promoter region of the IL-6 gene in patients with severe COVID-19 and patients with mild COVID-19.DISCUSSION: Our study indicated that these SNPs are not associated with COVID-19 severity in the Kurdish population from Kermanshah, Iran.
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