白鸟
(2023-07-31 22:58):
#paper https://doi.org/10.1038/s41586-023-06252-9 A spatially resolved single-cell genomic atlas of the adult human breast 该项目是陈-扎克伯格倡议（Chan Zuckerberg Initiative）支持的全球人类细胞图谱（Human Cell Atlas）联盟的一部分，该联盟利用最新技术为人体的每个器官系统构建细胞参考图谱。
研究目的：
人类乳腺的解剖学和组织病理学已经研究了几十年，为发育、哺乳和疾病提供了深入见解。最近，用分子和基因组技术对正常乳腺组织进行了表征，这些方法主要集中在上皮细胞上。迄今为止，仍然缺乏对所有细胞类型及其生物亚型（细胞状态）的无偏的全面解析。
研究意义：
1.HBCA图谱数据为研究乳腺生物学和乳腺癌等疾病状态提供了前所未有的成人正常乳腺组织的参考；
2.HBCA项目的所有单细胞和空间数据都可以通过官方的门户网站公开访问；
3. 人类细胞图谱（HCA，Human Cell Atlas）的一部分；
A spatially resolved single-cell genomic atlas of the adult human breast
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Abstract:
The adult human breast is comprised of an intricate network of epithelial ducts and lobules that are embedded in connective and adipose tissue. Although most previous studies have focused on the breast epithelial system, many of the non-epithelial cell types remain understudied. Here we constructed the comprehensive Human Breast Cell Atlas (HBCA) at single-cell and spatial resolution. Our single-cell transcriptomics study profiled 714,331 cells from 126 women, and 117,346 nuclei from 20 women, identifying 12 major cell types and 58 biological cell states. These data reveal abundant perivascular, endothelial and immune cell populations, and highly diverse luminal epithelial cell states. Spatial mapping using four different technologies revealed an unexpectedly rich ecosystem of tissue-resident immune cells, as well as distinct molecular differences between ductal and lobular regions. Collectively, these data provide a reference of the adult normal breast tissue for studying mammary biology and diseases such as breast cancer.
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