prayer
(2022-06-30 11:49):
#paper doi:10.1016/j.cell.2022.04.003; Cell, 2022, Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays：华大基因5月Cell发文，使用Stereo-seq技术（大视野，单细胞分辨率，灵敏度高）绘制了不同胚胎时期小鼠器官发生的时空转录组图谱。技术方法不是看的很懂，有待进一步学习。
附原始数据链接：CNP0001543（https://db.cngb.org/search/project/CNP0001543）
Spatiotemporal transcriptomic atlas of mouse organogenesis using DNA nanoball-patterned arrays
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Abstract:
Spatially resolved transcriptomic technologies are promising tools to study complex biological processes such as mammalian embryogenesis. However, the imbalance between resolution, gene capture, and field of view of current methodologies precludes their systematic application to analyze relatively large and three-dimensional mid- and late-gestation embryos. Here, we combined DNA nanoball (DNB)-patterned arrays and in situ RNA capture to create spatial enhanced resolution omics-sequencing (Stereo-seq). We applied Stereo-seq to generate the mouse organogenesis spatiotemporal transcriptomic atlas (MOSTA), which maps with single-cell resolution and high sensitivity the kinetics and directionality of transcriptional variation during mouse organogenesis. We used this information to gain insight into the molecular basis of spatial cell heterogeneity and cell fate specification in developing tissues such as the dorsal midbrain. Our panoramic atlas will facilitate in-depth investigation of longstanding questions concerning normal and abnormal mammalian development.
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