白鸟 (2025-01-31 11:26):
#paper 10.1126/sciadv.aba1972 Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis. 这是2020年发表一篇PF肺纤维化的文章，文章很经典，发现了一群稀有细胞群，KRT5 −/KRT17 +上皮细胞群， 产生ECM细胞外基质。文章的分析也很系统，单细胞的常规分析和湿实验验证，同类文章的稀有细胞验证。实验部分有个细节，流式分选CD45 −和 C45 +细胞以 2:1 的比例混合成单细胞悬液，故上皮细胞检出很多。目前，疾病相关的单细胞文章很多，对同类疾病文章之间结论，细节，反复梳理和验证，可能会有新的发现。
Science Advances, 2020-7-10. DOI: 10.1126/sciadv.aba1972
Single-cell RNA sequencing reveals profibrotic roles of distinct epithelial and mesenchymal lineages in pulmonary fibrosis
Arun C. Habermann, Austin J. Gutierrez, Linh T. Bui, Stephanie L. Yahn, Nichelle I. Winters, Carla L. Calvi, Lance Peter, Mei-I Chung, Chase J. Taylor, Christopher Jetter, ... >>>
Arun C. Habermann, Austin J. Gutierrez, Linh T. Bui, Stephanie L. Yahn, Nichelle I. Winters, Carla L. Calvi, Lance Peter, Mei-I Chung, Chase J. Taylor, Christopher Jetter, Latha Raju, Jamie Roberson, Guixiao Ding, Lori Wood, Jennifer M. S. Sucre, Bradley W. Richmond, Ana P. Serezani, Wyatt J. McDonnell, Simon B. Mallal, Matthew J. Bacchetta, James E. Loyd, Ciara M. Shaver, Lorraine B. Ware, Ross Bremner, Rajat Walia, Timothy S. Blackwell, Nicholas E. Banovich, Jonathan A. Kropski <<<
Abstract:
Single-cell RNA sequencing provides new insights into pathologic epithelial and mesenchymal remodeling in the human lung.
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