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颜林林
(2022-06-06 07:18):
#paper doi:10.1016/j.copbio.2022.102691 Current Opinion in Biotechnology, New opportunities for genetic code expansion in synthetic yeast. 这是一篇对基于酵母的人工从头构建的生物合成体系技术的综述和展望。为了探索生命的起源和演化机制等基本问题，人们很早就开始尝试从头合成生命，并且在这些年持续取得了大量进步，其中就包括完全人工合成酵母细胞的每一条染色体，并将它们装配起来，形成具有生物活性的人工酵母细胞体系。这个体系（合成酵母Sc2.0）目前已经接近完成，它将是第一个自下而上构建的真核生物。由于可以精确控制其遗传背景及其生物内环境，人们可以深入研究诸如密码子替换和引入非经典氨基酸等自然界中很难发生的场景，并由此精确控制蛋白质的结构和功能，包括解决诸如过量表达等常规细胞工程难以处理的实际问题，为开发基于蛋白质的治疗剂、材料和催化剂提供了新的机会。
IF:7.100Q1
Current opinion in biotechnology,
2022-06.
DOI: 10.1016/j.copbio.2022.102691
PMID: 35151980
Abstract:
The synthetic yeast, Sc2.0, is nearing completion as consolidation of all 17 synthetic chromosomes into a single cell advances. This organism will be the first synthetic eukaryote and provides a …
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The synthetic yeast, Sc2.0, is nearing completion as consolidation of all 17 synthetic chromosomes into a single cell advances. This organism will be the first synthetic eukaryote and provides a highly plastic biological chassis built from the bottom-up using principles of biological design. This synthetic approach to genome construction has allowed the genetic code to be re-wired in this background to liberate the amber stop codon as a dedicated triplet for encoding non-canonical amino acids. The availability of an expanded set of amino acid building blocks allows precise control of protein structure and function, providing new opportunities to develop protein-based therapeutics, materials and catalysts. In this article, we review the challenges facing genetic code expansion research in yeast and highlight how the development of Sc2.0 provides new and exciting opportunities to address existing limitations.
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