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惊鸿
(2023-06-25 16:56):
#paper https://doi.org/10.1021/acssynbio.3c00216 Immortalized Bovine Satellite Cells for Cultured Meat Applications 为了使培养肉大规模成功，来自食品相关物种的肌肉细胞必须以快速可靠的方式在体外扩增，以每年产生数百万公吨的生物质。为了实现这一目标，遗传永生化细胞比原代细胞具有实质性的好处，包括快速生长、逃离细胞衰老以及一致的起始细胞群进行生产。在这里，我们通过牛端粒酶逆转录酶（TERT）和细胞周期蛋白依赖性激酶4（CDK4）的组成表达开发遗传永生化的牛卫星细胞（iBSC）。这些细胞在发表时实现了超过120倍的倍增，并保持其肌源分化的能力。因此，它们为该领域提供了有价值的工具，使进一步的研究和开发能够推进培养肉。
Abstract:
For cultured meat to succeed at scale, muscle cells from food-relevant species must be expanded in vitro in a rapid and reliable manner to produce millions of metric tons of …
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For cultured meat to succeed at scale, muscle cells from food-relevant species must be expanded in vitro in a rapid and reliable manner to produce millions of metric tons of biomass annually. Toward this goal, genetically immortalized cells offer substantial benefits over primary cells, including rapid growth, escape from cellular senescence, and consistent starting cell populations for production. Here, we develop genetically immortalized bovine satellite cells (iBSCs) via constitutive expression of bovine Telomerase reverse transcriptase (TERT) and Cyclin-dependent kinase 4 (CDK4). These cells achieve over 120 doublings at the time of publication and maintain their capacity for myogenic differentiation. They therefore offer a valuable tool to the field, enabling further research and development to advance cultured meat.
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