2021,
Proceedings of the National Academy of Sciences.
DOI:
10.1073/pnas.2105968118
Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues
Liguo Zhang
, Alexsia Richards
, M. Inmaculada Barrasa
, Stephen H. Hughes
, Richard A. Young
, Rudolf Jaenisch
Abstract:
Significance
An unresolved issue of SARS-CoV-2 disease is that patients often remain positive for viral RNA as detected by PCR many weeks after the initial infection in the absence of evidence for viral replication. We show here that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of the infected cell and be expressed as chimeric transcripts fusing viral with cellular sequences. Importantly, such chimeric transcripts are detected in patient-derived tissues. Our data suggest that, in some patient tissues, the majority of all viral transcripts are derived from integrated sequences. Our data provide an insight into the consequence of SARS-CoV-2 infections that may help to explain why patients can continue to produce viral RNA after recovery.
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2022-12-31 19:23:00
钟鸣:
#paper Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues https://doi.org/10.1073/pnas.2105968118
许多新冠感染者康复后依然能通过PCR检测到病毒，但是却分离不到活病毒，考虑到新冠病毒是一种RNA病毒，因此合理怀疑其逆转录整合到了宿主基因组上。为了证实，作者使用了三种测序方法来对人类细胞来源的基因组测序，结果均发现了新冠基因组。此外作者还回顾了感染者组织来源的RNA测序数据，发现了包括新冠在内的许多病毒的转录本。比较简单的一篇论文，比较可看的地方是讨论。