2022,
Experimental Cell Research.
DOI:
10.1016/j.yexcr.2022.113163
Physiological levels of poly(ADP-ribose) during the cell cycle regulate HeLa cell proliferation
Sachiko Yamashita, Masakazu Tanaka, Chieri Ida, Kenichi Kouyama, Setsu Nakae, Taisuke Matsuki, Masataka Tsuda, Tsuyoshi Shirai, Kazuo Kamemura, Yoshisuke Nishi, Joel Moss, Masanao Miwa
Abstract:
No abstract available.
Related Links:
2024-04-01 09:07:00
龙海晨:
#paper Yamashita S, Tanaka M, Ida C, Kouyama K, Nakae S, Matsuki T, Tsuda M, Shirai T, Kamemura K, Nishi Y, Moss J, Miwa M. Physiological levels of poly(ADP-ribose) during the cell cycle regulate HeLa cell proliferation. Exp Cell Res. 2022 Aug 1;417(1):113163. doi: 10.1016/j.yexcr.2022.113163. Epub 2022 Apr 18. PMID: 35447104; PMCID: PMC10009817. PAR: Poly(ADP-ribose) ;PARG Poly(ADP-ribose) glycohydrolase ; PARP Poly(ADP-ribose) polymerase ;该文章在在不使用PARG抑制剂的情况下量化了HeLa细胞整个细胞周期内PAR的基本水平，发现S期的PAR水平显着高于G1期，其次非应激条件下PAR的半衰期小于40秒，这与S期大量NAD +的消耗一致，这是由PARP抑制剂挽救的。第三，PARP抑制剂使细胞周期延迟至S期。第四，S期期间PAR水平的增加与PARP1、PARG、NAD +和pERK2的水平无关。第五，纯化的 PARP1 在 DNA 不存在的情况下被组蛋白 H4 激活。PARP 抑制剂延迟 S 期细胞周期并减少细胞增殖。